A4IPP2 (AROD_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	GTNG_1941

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325525
Sites
	Active site	147	1	Proton acceptor By similarity
	Active site	174	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IPP2 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: C760535029A8B3BF	FASTA	257	28,151
	10 20 30 40 50 60 MAISKGAINV RSIFIGGEEP CICAPVVGVD AEQVLAETRQ ISAKKPHLIE WRADFFNGIH 70 80 90 100 110 120 DDRQVVATAK EIRTIAGDIP ILFTVRSERE GGRPIPLTEE EKMRLFETVC QSGAIDLLDY 130 140 150 160 170 180 ELVHEPYVAT VRQLSRQYGV RLILSYHNFD FTPAKEELVA KMRQAGKYGA DIAKVAVMPK 190 200 210 220 230 240 SLQDVLVLLQ ATEEARQELP IPLITMSMGG LGAITRLAGG LFGSAVTFAV GQQSSAPGQI 250 PIEEVKDVLS VIMKYSQ

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO67296.1.
RefSeq	YP_001126041.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IPP2.
SMR	A4IPP2. Positions 1-257.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IPP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4966871.
GenomeReviews	Gene locus GTNG_1941 in contig CP000557_GR.
KEGG	gtn:GTNG_1941.
NMPDR	fig|420246.5.peg.1866.
PATRIC	21980357. VBIGeoThe136879_2047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HBG732926.
OMA	SYHNFDE.
PhylomeDB	A4IPP2.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	GTHE420246:GTNG_1941-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_GEOTN

Accession

Primary (citable) accession number: A4IPP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program