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A4IPF5 (IOLA2_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylmalonate semialdehyde dehydrogenase [acylating] 2
Short name=MMSA dehydrogenase 2
Short name=MMSDH 2
Short name=MSDH 2
EC=1.2.1.27
Alternative name(s):
Malonate semialdehyde dehydrogenase [acetylating] 2
Short name=MSA dehydrogenase 2
EC=1.2.1.18
Gene names
Name:iolA2
Ordered Locus Names:GTNG_1849
OrganismGeobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]
Taxonomic identifier420246 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity. HAMAP MF_01670

Catalytic activity

3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670

Pathway

Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670

Subunit structure

Homotetramer By similarity. HAMAP MF_01670

Sequence similarities

Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Sequence caution

The sequence ABO67209.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670
PRO_0000352343

Regions

Nucleotide binding176 – 1805NAD By similarity

Sites

Active site2841Nucleophile By similarity
Binding site3841NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A4IPF5 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 34C29260FE174418

FASTA48352,812
        10         20         30         40         50         60 
MTETKTLKNF IGGQWVASTS GKEEIVPNPA TGEVLAKVPI SSREELDAAV AAAKEAFREW 

        70         80         90        100        110        120 
RKVPVPRRAR ILFRYQQLLV EHWEELARLV TLENGKVYED AYGEVQRGIE CVEFAAGIPT 

       130        140        150        160        170        180 
LMMGQQLPDI ATGIESGMYR YPLGVVAGIT PFNFPMMVPC WMFPLAIACG NTFVLKPSER 

       190        200        210        220        230        240 
TPLLANRLAE LFTEAGLPAG VLNIVHGAHE VVNGILEHKD IKAVSFVGSQ PVAEYVYKTA 

       250        260        270        280        290        300 
AAYGKRVQAL AGAKNHSIVM PDADLDMAVT NIINAAFGSA GERCMACSVV VAVGDIADEL 

       310        320        330        340        350        360 
VERLKKAADR IQIGNGLDQG VFLGPVIRES HKERTIKYIE IGEREGALLV RDGRRDAATS 

       370        380        390        400        410        420 
GKGYFVGPTI FDHVKPGMTI WTDEIFAPVL SVVRARDLDE AIEIANRSEF ANGACIYTDS 

       430        440        450        460        470        480 
AKAIRQFREE IDAGMLGVNV AVPAPMAFFP FSGYKNSFYG DLHANGRDGV EFYTRKKMVT 


ARH

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References

[1]"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NG80-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000557 Genomic DNA. Translation: ABO67209.1. Different initiation.
RefSeqYP_001125954.1. NC_009328.1.

3D structure databases

ProteinModelPortalA4IPF5.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4IPF5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4966782.
GenomeReviewsGene locus GTNG_1849 in contig CP000557_GR.
KEGGgtn:GTNG_1849.
NMPDRfig|420246.5.peg.1779.
PATRIC21980163. VBIGeoThe136879_1950.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHBG752218.
ProtClustDBCLSK2524240.

Enzyme and pathway databases

BioCycGTHE420246:GTNG_1849-MONOMER.

Family and domain databases

HAMAPMF_01670. IolA.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
IPR023510. MeMal-semiAld_DH_GmP_bac.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KOK00140.
PANTHERPTHR11699:SF27. MMSDH. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR01722. MMSDH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIOLA2_GEOTN
AccessionPrimary (citable) accession number: A4IPF5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 14, 2008
Last modified: December 14, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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