Reviewed, UniProtKB/Swiss-Prot A4IPF5 (IOLA2_GEOTN)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 2
      Short name=MMSA dehydrogenase 2
      Short name=MMSDH 2
      Short name=MSDH 2
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 2
      Short name=MSA dehydrogenase 2
    EC=1.2.1.18

Gene names

Name:	iolA2
Ordered Locus Names:	GTNG_1849

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670		PRO_0000352343
Regions
	Nucleotide binding	176 – 180	5	NAD By similarity
Sites
	Active site	284	1	Nucleophile By similarity
	Binding site	384	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IPF5-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 34C29260FE174418	FASTA	483	52,812
	10 20 30 40 50 60 MTETKTLKNF IGGQWVASTS GKEEIVPNPA TGEVLAKVPI SSREELDAAV AAAKEAFREW 70 80 90 100 110 120 RKVPVPRRAR ILFRYQQLLV EHWEELARLV TLENGKVYED AYGEVQRGIE CVEFAAGIPT 130 140 150 160 170 180 LMMGQQLPDI ATGIESGMYR YPLGVVAGIT PFNFPMMVPC WMFPLAIACG NTFVLKPSER 190 200 210 220 230 240 TPLLANRLAE LFTEAGLPAG VLNIVHGAHE VVNGILEHKD IKAVSFVGSQ PVAEYVYKTA 250 260 270 280 290 300 AAYGKRVQAL AGAKNHSIVM PDADLDMAVT NIINAAFGSA GERCMACSVV VAVGDIADEL 310 320 330 340 350 360 VERLKKAADR IQIGNGLDQG VFLGPVIRES HKERTIKYIE IGEREGALLV RDGRRDAATS 370 380 390 400 410 420 GKGYFVGPTI FDHVKPGMTI WTDEIFAPVL SVVRARDLDE AIEIANRSEF ANGACIYTDS 430 440 450 460 470 480 AKAIRQFREE IDAGMLGVNV AVPAPMAFFP FSGYKNSFYG DLHANGRDGV EFYTRKKMVT ARH

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000557 Genomic DNA. Translation: ABO67209.1. Different initiation.
RefSeq	YP_001125954.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4966782.
GenomeReviews	Gene locus GTNG_1849 in contig CP000557_GR.
KEGG	gtn:GTNG_1849.
NMPDR	fig|420246.5.peg.1779.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA2_GEOTN

Accession

Primary (citable) accession number: A4IPF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	June 16, 2009
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)