Reviewed, UniProtKB/Swiss-Prot A4IPB6 (IOLD_GEOTN)

Names and origin

Protein names

Recommended name:
    3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
      Short name=THcHDO hydrolase
    EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	GTNG_1810

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity.
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352543
Regions
	Region	437 – 517	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	488	1	Magnesium By similarity
	Metal binding	515	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IPB6-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 1F81E53C52CC5619	FASTA	639	69,908
	10 20 30 40 50 60 MSTIRLTTAQ ALVKFLNNQY VEFDGKQQRF VKGIFTIFGH GNVLGLGQAL EEDPGELEVY 70 80 90 100 110 120 QGRNEQGMAN AAIAFAKQKN RKQIMAATSS VGPGAANMVT SAATATANNI PVLLLPGDVF 130 140 150 160 170 180 ATRQPDPVLQ QIEHTHDLSI STNDAFRAVS KYWDRVCRPE QLMSAMLNAM RVLTNPADTG 190 200 210 220 230 240 AVTIALPQDV QGEAWDFPES FFAKRVHRIE RRQPSLESVR DAVKLIRSKK KPLLVLGGGV 250 260 270 280 290 300 RYSEAADAFV KFAEKFNIPF AETQAGKGTI ESSHPLNLGG IGVTGNLAAN TIAKQADLII 310 320 330 340 350 360 GVGTRFTDFT TASKQLFSSA EILTINVSEF HASKLDAVKV VADAKAGLEA IAEALGDYVS 370 380 390 400 410 420 AYGNEISEAK QAWNRELERL CSVAYGENFT PEIAGHLDEK LPEYREAFGS ELTQTGVIGK 430 440 450 460 470 480 VNELIDDDAV IVGASGSLPG DLQRMWVCKD RNTYHMEYGY SCMGYEIAGA FGVKLAEPDK 490 500 510 520 530 540 EVYAMVGDGS FLMLHSELVT SLQEGQKINI ILFDNSGFGC INNLQMENGM GSFVTEFRKR 550 560 570 580 590 600 NLETGQLDGP IMTIDYAKVA EGYGLKTYSV RTMEELETAL IDSKKQSIST LIDIKVLPKT 610 620 630 MTHGYGSWWH VGVAEVSTKE SVQAAYRNKQ ENLKLARKY

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000557 Genomic DNA. Translation: ABO67170.1.
RefSeq	YP_001125915.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4966743.
GenomeReviews	Gene locus GTNG_1810 in contig CP000557_GR.
KEGG	gtn:GTNG_1810.
NMPDR	fig|420246.5.peg.1742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4IPB6. GELEVYQ.
Family and domain databases
HAMAP	MF_01669. [Tree]
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_GEOTN

Accession

Primary (citable) accession number: A4IPB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)