Reviewed, UniProtKB/Swiss-Prot A4IP94 (G1PDH_GEOTN)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	GTNG_1788

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species By similarity. HAMAP MF_00497
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 nickel ion per subunit By similarity. HAMAP MF_00497
Subunit structure	Homodimer By similarity. HAMAP MF_00497
Subcellular location	Cytoplasm Potential HAMAP MF_00497.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350641
Regions
	Nucleotide binding	117 – 121	5	NAD By similarity
	Nucleotide binding	139 – 142	4	NAD By similarity
Sites
	Metal binding	191	1	Nickel; catalytic By similarity
	Metal binding	271	1	Nickel; catalytic By similarity
	Metal binding	291	1	Nickel; catalytic By similarity
	Binding site	55	1	NAD By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	148	1	NAD By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IP94-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: A30A90E9115BB3B0	FASTA	404	45,328
	10 20 30 40 50 60 MNQTLDNIVS LAKQCRGHHH YDISIEQIVV SHEAFNQLAA YLRYKQYKKV VVVADNRTFT 70 80 90 100 110 120 AAGRSLCDKL KNESVLYTVC LIQSDENEDV IADERAIMQV LLETPNDVDA FIAVGAGTVH 130 140 150 160 170 180 DITRFSSYKM KVPFISVPTA PSVDGFTSMG APLIIRGVKK TIQAQAPIAV FADTDVLCQA 190 200 210 220 230 240 PKAMIAAGFG DMVAKYTSLA DWQFAHWMAN EPYCPLVYQL TIQSLNACVD HMEDIAAGNE 250 260 270 280 290 300 KGIHVLMDAL LQSGIAMLLM GQSYSASGAE HHLSHYWEME FLRQNKRQVL HGAKVGVSTP 310 320 330 340 350 360 IIVEHYQRVF WPLLSELKQR PKSMDETIWE RLKIYTDSIQ ELLESLPSPE RIRAMVEKVG 370 380 390 400 GAVAPQQLGI DPLLVERSLK EAHRLRLNRF TMLYCLNECM LMEG

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO67148.1.
RefSeq	YP_001125893.1.
3D structure databases
SMR	A4IP94. Positions 27-393.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IP94.
Genome annotation databases
GeneID	4966721.
GenomeReviews	Gene locus GTNG_1788 in contig CP000557_GR.
KEGG	gtn:GTNG_1788.
NMPDR	fig|420246.5.peg.1720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0371.
HOGENOM	HBG313183.
OMA	NIRERFT.
Family and domain databases
HAMAP	MF_00497_B. G1P_dehydrogenase_B. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G1PDH_GEOTN

Accession

Primary (citable) accession number: A4IP94

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 1, 2007
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)