A4INZ5 (A4INZ5_GEOTN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027

Gene names

Name:	cbiA EMBL ABO67049.1
Synonyms:	cobB HAMAP-Rule MF_00027
Ordered Locus Names:	GTNG_1685 EMBL ABO67049.1

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP] EMBL ABO67049.1

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Bacillus thermodenitrificans

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. HAMAP-Rule MF_00027 Contains 1 GATase cobBQ-type domain. HAMAP-Rule MF_00027 SAAS SAAS011698

Ontologies

Keywords
Biological process	Cobalamin biosynthesis HAMAP-Rule MF_00027 SAAS SAAS011698
Domain	Glutamine amidotransferase HAMAP-Rule MF_00027 SAAS SAAS011698
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	246 – 438	193	GATase cobBQ-type By similarity HAMAP-Rule MF_00027
Sites
	Active site	329	1	Nucleophile By similarity HAMAP-Rule MF_00027
	Active site	430	1	By similarity HAMAP-Rule MF_00027

Sequences

	Sequence		Length	Mass (Da)
	A4INZ5 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 9DBC926F49E5B328	FASTA	454	48,640
	10 20 30 40 50 60 MRRLVIAAPG SGAGKTTVTL GLMAAMRQQG YTVQGFKCGP DYIDPTYHTA VTGRPARNLD 70 80 90 100 110 120 SWMMGSEAVK TVLANGCQGA DIAIIEGVMG LFDGKQPLSD EGSTAEIAVL TESPVLLVVD 130 140 150 160 170 180 CSGMARSAAA IVHGFQTFRP DVRLAGVFAN RVGSEGHFRL VKAAVEQTCG VPVVGFLTQD 190 200 210 220 230 240 EALALPERHL GLVPSVERGE LDSFFAELGR KVARTIDWET LLSIAEAPAL CSPAPLIRPS 250 260 270 280 290 300 RPYRVRVAVA KDAAFHFYYP ENLEMLSACG AELVYFSPLA GEPLPDGVNG LYIGGGFPEE 310 320 330 340 350 360 FAAELARQAA VKQSIRAAIE HGLPTLAECG GFMFLTEQLV ATDGAAYEMA GVIPGKVVMK 370 380 390 400 410 420 TKLAALGYRE VRGRNGNFLL PEGETARGHE FHYSVYEPRG EAPFAYETSG RKGTKPDGYL 430 440 450 AHRLVAGYVH FHFASAPAMV ERWLAECEKV TING

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2 EMBL ABO67049.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO67049.1.
RefSeq	YP_001125794.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4INZ5.
ModBase	Search...
Protein-protein interaction databases
STRING	420246.GTNG_1685.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO67049; ABO67049; GTNG_1685.
GeneID	4966621.
KEGG	gtn:GTNG_1685.
PATRIC	21979829. VBIGeoThe136879_1784.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289959.
KO	K02224.
OMA	FIDPSHH.
ProtClustDB	PRK01077.
Enzyme and pathway databases
BioCyc	GTHE420246:GIXT-1764-MONOMER.
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4INZ5_GEOTN

Accession

Primary (citable) accession number: A4INZ5

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)