Reviewed, UniProtKB/Swiss-Prot A4INR2 (ARSC_GEOTN)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:	arsC
Ordered Locus Names:	GTNG_1600

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Protein arsC HAMAP MF_01624		PRO_1000073636
Sites
	Active site	11	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	83	1	Nucleophile; for reductase activity By similarity
	Active site	90	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 83		Redox-active; alternate By similarity
	Disulfide bond	83 ↔ 90		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4INR2-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 7E48B42880FB66AD	FASTA	140	15,545
	10 20 30 40 50 60 MKNKKIIYFL CTGNSCRSQM AEGWAKKYLG DDWEVYSAGI EAHGLNPNAV KVMKEVGIDI 70 80 90 100 110 120 SNQTSDVIDP EILNKADLVV TLCGHAADHC PVTPPNVKRV HWGFDDPAKA EGTEEEKLAV 130 140 FRRVRDEIGA RIKKFAETGE

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000557 Genomic DNA. Translation: ABO66966.1.
RefSeq	YP_001125711.1.
3D structure databases
SMR	A4INR2. Positions 5-140.
ModBase	Search...
Protein-protein interaction databases
STRING	A4INR2.
Genome annotation databases
GeneID	4966537.
GenomeReviews	Gene locus GTNG_1600 in contig CP000557_GR.
KEGG	gtn:GTNG_1600.
NMPDR	fig|420246.5.peg.1543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EMRIVIT.
Family and domain databases
HAMAP	MF_01624. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_GEOTN

Accession

Primary (citable) accession number: A4INR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 1, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)