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A4IN50

- MASZ_GEOTN

UniProt

A4IN50 - MASZ_GEOTN

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Protein

Malate synthase G

Gene
glcB, GTNG_1384
Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei275 – 2751Acetyl-CoA By similarity
Binding sitei312 – 3121Acetyl-CoA By similarity
Active sitei339 – 3391Proton acceptor By similarity
Binding sitei339 – 3391Glyoxylate By similarity
Metal bindingi431 – 4311Magnesium By similarity
Binding sitei431 – 4311Glyoxylate By similarity
Metal bindingi459 – 4591Magnesium By similarity
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei630 – 6301Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-1456-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:GTNG_1384
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001578: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GUniRule annotationPRO_1000056903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi420246.GTNG_1384.

Structurei

3D structure databases

ProteinModelPortaliA4IN50.
SMRiA4IN50. Positions 5-723.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA binding By similarity
Regioni456 – 4594Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A4IN50-1 [UniParc]FASTAAdd to Basket

« Hide

MIEYVQAGTI QVAKVLYEFV NEELLPNSGL DQDKFWSDFA ALIADLTPRN    50
KELLARRDEI QEKLNEWYKE HRGRFDFHEY KAFLTDIGYL EPEVEDFEIT 100
TDNVDEEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE 150
DGAERGSSYN PVRGAKVIAY GRQFLDEAVP LVEHSHKDAV QYAIVDGKLV 200
VTTEGGATTG LKEPEKLIGF QGEPQNPTAV LLKNNGLHIE IQIDREHPVG 250
KTDKAGIKDI VLEAAVTTIM DGEDSVAAVD AEDKVVVYRN LFGLIKGDLT 300
ATFEKNGKRL TRTLNPDREY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI 350
LDANGEEVYE GIIDAVVTSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS 400
AEVAFANELF DRVEDMLGLE RNTIKIGVMD EERRTSLNLK NCIYEVRDRI 450
VFINTGFLDR TGDEIHTSME AGPMRRKNDM KSSTWLAGYE KSNVAVGLAA 500
GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP SPTAATLHAL 550
HYHQVNVAAV QNELANDRND YRDDILQIPV VDNPQWTADE IQEELDNNCQ 600
SILGYVVRWI DQGIGCSKVP DIHNIGLMED RATLRISSQI LANWLHHGIC 650
TKEQVLETFK RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLVFLGY 700
EQPNGYTEPI LHRRRQEAKA KFAAVQQ 727
Length:727
Mass (Da):81,200
Last modified:May 1, 2007 - v1
Checksum:i37B1B2D489B3ADA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO66754.1.
RefSeqiYP_001125499.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO66754; ABO66754; GTNG_1384.
GeneIDi4966323.
KEGGigtn:GTNG_1384.
PATRICi21979151. VBIGeoThe136879_1455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO66754.1 .
RefSeqi YP_001125499.1. NC_009328.1.

3D structure databases

ProteinModelPortali A4IN50.
SMRi A4IN50. Positions 5-723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 420246.GTNG_1384.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO66754 ; ABO66754 ; GTNG_1384 .
GeneIDi 4966323.
KEGGi gtn:GTNG_1384.
PATRICi 21979151. VBIGeoThe136879_1455.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci GTHE420246:GIXT-1456-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
    Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
    Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NG80-2.

Entry informationi

Entry nameiMASZ_GEOTN
AccessioniPrimary (citable) accession number: A4IN50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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