A4IN50 (MASZ_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	GTNG_1384

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Malate synthase G HAMAP MF_00641		PRO_1000056903
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	630	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IN50 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 37B1B2D489B3ADA2	FASTA	727	81,200
	10 20 30 40 50 60 MIEYVQAGTI QVAKVLYEFV NEELLPNSGL DQDKFWSDFA ALIADLTPRN KELLARRDEI 70 80 90 100 110 120 QEKLNEWYKE HRGRFDFHEY KAFLTDIGYL EPEVEDFEIT TDNVDEEIAV QAGPQLVVPL 130 140 150 160 170 180 TNARYALNAA NARWGSLYDA LYGTDAISEE DGAERGSSYN PVRGAKVIAY GRQFLDEAVP 190 200 210 220 230 240 LVEHSHKDAV QYAIVDGKLV VTTEGGATTG LKEPEKLIGF QGEPQNPTAV LLKNNGLHIE 250 260 270 280 290 300 IQIDREHPVG KTDKAGIKDI VLEAAVTTIM DGEDSVAAVD AEDKVVVYRN LFGLIKGDLT 310 320 330 340 350 360 ATFEKNGKRL TRTLNPDREY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI LDANGEEVYE 370 380 390 400 410 420 GIIDAVVTSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS AEVAFANELF DRVEDMLGLE 430 440 450 460 470 480 RNTIKIGVMD EERRTSLNLK NCIYEVRDRI VFINTGFLDR TGDEIHTSME AGPMRRKNDM 490 500 510 520 530 540 KSSTWLAGYE KSNVAVGLAA GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP 550 560 570 580 590 600 SPTAATLHAL HYHQVNVAAV QNELANDRND YRDDILQIPV VDNPQWTADE IQEELDNNCQ 610 620 630 640 650 660 SILGYVVRWI DQGIGCSKVP DIHNIGLMED RATLRISSQI LANWLHHGIC TKEQVLETFK 670 680 690 700 710 720 RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLVFLGY EQPNGYTEPI LHRRRQEAKA KFAAVQQ

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO66754.1.
RefSeq	YP_001125499.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IN50.
SMR	A4IN50. Positions 5-723.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IN50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4966323.
GenomeReviews	Gene locus GTNG_1384 in contig CP000557_GR.
KEGG	gtn:GTNG_1384.
NMPDR	fig|420246.5.peg.1342.
PATRIC	21979151. VBIGeoThe136879_1455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	A4IN50.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	GTHE420246:GTNG_1384-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_GEOTN

Accession

Primary (citable) accession number: A4IN50

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program