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A4IN50

- MASZ_GEOTN

UniProt

A4IN50 - MASZ_GEOTN

Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi431 – 4311MagnesiumUniRule annotation
    Binding sitei431 – 4311GlyoxylateUniRule annotation
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei630 – 6301Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciGTHE420246:GIXT-1456-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:GTNG_1384
    OrganismiGeobacillus thermodenitrificans (strain NG80-2)
    Taxonomic identifieri420246 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000001578: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_1000056903Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi420246.GTNG_1384.

    Structurei

    3D structure databases

    ProteinModelPortaliA4IN50.
    SMRiA4IN50. Positions 5-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni456 – 4594Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4IN50-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEYVQAGTI QVAKVLYEFV NEELLPNSGL DQDKFWSDFA ALIADLTPRN    50
    KELLARRDEI QEKLNEWYKE HRGRFDFHEY KAFLTDIGYL EPEVEDFEIT 100
    TDNVDEEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE 150
    DGAERGSSYN PVRGAKVIAY GRQFLDEAVP LVEHSHKDAV QYAIVDGKLV 200
    VTTEGGATTG LKEPEKLIGF QGEPQNPTAV LLKNNGLHIE IQIDREHPVG 250
    KTDKAGIKDI VLEAAVTTIM DGEDSVAAVD AEDKVVVYRN LFGLIKGDLT 300
    ATFEKNGKRL TRTLNPDREY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI 350
    LDANGEEVYE GIIDAVVTSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS 400
    AEVAFANELF DRVEDMLGLE RNTIKIGVMD EERRTSLNLK NCIYEVRDRI 450
    VFINTGFLDR TGDEIHTSME AGPMRRKNDM KSSTWLAGYE KSNVAVGLAA 500
    GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP SPTAATLHAL 550
    HYHQVNVAAV QNELANDRND YRDDILQIPV VDNPQWTADE IQEELDNNCQ 600
    SILGYVVRWI DQGIGCSKVP DIHNIGLMED RATLRISSQI LANWLHHGIC 650
    TKEQVLETFK RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLVFLGY 700
    EQPNGYTEPI LHRRRQEAKA KFAAVQQ 727
    Length:727
    Mass (Da):81,200
    Last modified:May 1, 2007 - v1
    Checksum:i37B1B2D489B3ADA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO66754.1.
    RefSeqiYP_001125499.1. NC_009328.1.

    Genome annotation databases

    EnsemblBacteriaiABO66754; ABO66754; GTNG_1384.
    GeneIDi4966323.
    KEGGigtn:GTNG_1384.
    PATRICi21979151. VBIGeoThe136879_1455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO66754.1 .
    RefSeqi YP_001125499.1. NC_009328.1.

    3D structure databases

    ProteinModelPortali A4IN50.
    SMRi A4IN50. Positions 5-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 420246.GTNG_1384.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO66754 ; ABO66754 ; GTNG_1384 .
    GeneIDi 4966323.
    KEGGi gtn:GTNG_1384.
    PATRICi 21979151. VBIGeoThe136879_1455.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci GTHE420246:GIXT-1456-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
      Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
      Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NG80-2.

    Entry informationi

    Entry nameiMASZ_GEOTN
    AccessioniPrimary (citable) accession number: A4IN50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3