Reviewed, UniProtKB/Swiss-Prot A4IM31 (PYRC_GEOTN)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	GTNG_1007

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Dihydroorotase		PRO_1000024085
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1; via carbamate group By similarity
	Metal binding	143	1	Zinc 2; via carbamate group By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	233	1	Zinc 2 By similarity
	Metal binding	306	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	143	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IM31-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: BD249EB5D54F76BB	FASTA	428	46,453
	10 20 30 40 50 60 MAMWLKNGMS FNENGQLVRT HIKIEHGNIA AIHHEQLFEA NGEDVIDVGG KLIAPGLIDV 70 80 90 100 110 120 HVHLREPGGE AKETIETGTL AAAKGGFTTV AAMPNTNPVP DRKEQMEWLA RRIQETAHVR 130 140 150 160 170 180 VLPYASITLG QKGEELTDFA ALKEAGAFAF TDDGVGVQSA GMMFEAMKRA AALDMAIVAH 190 200 210 220 230 240 CEDDTLKNGG AVHDGDFARR YGIAGIPSVC EAVHIARDVL LAEATGCHYH VCHISTKESV 250 260 270 280 290 300 RVVRDAKRAG ICVTAEVTPH HLLLCDEDIP RLDANYKMNP PLRSRADREA LIEGLLDGTI 310 320 330 340 350 360 DFIATDHAPH TAAEKAKGME AAPFGIVGLE TAFPLLYTHF VKKNVFTLKQ LVDWLTIKPA 370 380 390 400 410 420 QCFGLQTGRL EVGAPADITV IDLETEEPID PETFASKGNN TPFAGWRCQG WPVMTFVGGT LVWEKGRA

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000557 Genomic DNA. Translation: ABO66385.1.
RefSeq	YP_001125130.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M38.972.
Genome annotation databases
GeneID	4965950.
GenomeReviews	Gene locus GTNG_1007 in contig CP000557_GR.
KEGG	gtn:GTNG_1007.
NMPDR	fig|420246.5.peg.976.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_GEOTN

Accession

Primary (citable) accession number: A4IM31

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	September 2, 2008
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)