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A4IM25

- SYI_GEOTN

UniProt

A4IM25 - SYI_GEOTN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi891 – 8911ZincUniRule annotation
    Metal bindingi894 – 8941ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi914 – 9141ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciGTHE420246:GIXT-1073-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:GTNG_1001
    OrganismiGeobacillus thermodenitrificans (strain NG80-2)
    Taxonomic identifieri420246 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000001578: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 924924Isoleucine--tRNA ligasePRO_1000022071Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi420246.GTNG_1001.

    Structurei

    3D structure databases

    ProteinModelPortaliA4IM25.
    SMRiA4IM25. Positions 2-871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4IM25-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLLMP QTEFPMRGNL PKREPEMQKK WEEMDIYRKV QERTKGRPLF    50
    VLHDGPPYAN GDIHMGHALN KILKDIIVRY KSMSGFCAPY VPGWDTHGLP 100
    IETALTKQGV DRKSMSVAEF RKLCEQYAYE QIDNQRQQFK RLGVRGDWDN 150
    PYITLKPEYE AQQIKVFGEM AKKGLIYKGL KPVYWSPSSE SALAEAEIEY 200
    KDKRSPSIYV AFPVKDGKGV LQGDERIVIW TTTPWTIPAN LAIAVHPDLD 250
    YYIVEANGQK YVVAAALAES VAKEVGWEAW SVVKTVKGKE LEYVVAKHPF 300
    YERDSLVVCG EHVTTDAGTG CVHTAPGHGE DDFIVGQKYG LPVLCPVDER 350
    GYMTEEAPGF AGMFYDEANK AITQKLEEVG ALLKLSFITH SYPHDWRTKQ 400
    PTIFRATTQW FASIDKIRDQ LLDAIKETKW VPEWGEIRIH NMVRDRGDWC 450
    ISRQRAWGVP IPVFYGENGE PIITDETIEH VSNLFRQYGS NVWFEREAKD 500
    LLPEGFTHPS SPNGLFTKET DIMDVWFDSG SSHQAVLVER DDLERPADLY 550
    LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSLGN 600
    VVVPAKVMEQ LGADILRLWV ASVDYQADVR ISDNILKQVS EVYRKIRNTF 650
    RFMLGNLFDF DPNQNAVPVG ELGELDRYML AKLNKLIAKV KKAYDSYDFA 700
    AVYHEMNHFC TVELSAFYLD MAKDILYIEA ADCRARRAVQ TVLYETVVAL 750
    AKLIAPILPH TADEVWEHIP NRKEQVESVQ LTDMPEPMAI DGEEALLAKW 800
    DAFMDVRDDI LKALENARNE KVIGKSLTAS VTVYPKDEVR ALLASINEDL 850
    RQLLIVSAFS VADESYDAAP AEAERLNHVA VIVRPAEGET CERCWTVTPD 900
    VGRDESHPTL CPRCAHIVNE HYSA 924
    Length:924
    Mass (Da):104,675
    Last modified:May 1, 2007 - v1
    Checksum:i1E5311B4A319A8AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO66379.1.
    RefSeqiWP_011887108.1. NC_009328.1.
    YP_001125124.1. NC_009328.1.

    Genome annotation databases

    EnsemblBacteriaiABO66379; ABO66379; GTNG_1001.
    GeneIDi4965944.
    KEGGigtn:GTNG_1001.
    PATRICi21978339. VBIGeoThe136879_1053.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO66379.1 .
    RefSeqi WP_011887108.1. NC_009328.1.
    YP_001125124.1. NC_009328.1.

    3D structure databases

    ProteinModelPortali A4IM25.
    SMRi A4IM25. Positions 2-871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 420246.GTNG_1001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO66379 ; ABO66379 ; GTNG_1001 .
    GeneIDi 4965944.
    KEGGi gtn:GTNG_1001.
    PATRICi 21978339. VBIGeoThe136879_1053.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci GTHE420246:GIXT-1073-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
      Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
      Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NG80-2.

    Entry informationi

    Entry nameiSYI_GEOTN
    AccessioniPrimary (citable) accession number: A4IM25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3