Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552Aminoacyl-adenylateUniRule annotation1
Binding sitei596ATPUniRule annotation1
Metal bindingi891ZincUniRule annotation1
Metal bindingi894ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi914ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGTHE420246:G1G82-1101-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:GTNG_1001
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001578 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220711 – 924Isoleucine--tRNA ligaseAdd BLAST924

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi420246.GTNG_1001

Structurei

3D structure databases

ProteinModelPortaliA4IM25
SMRiA4IM25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A4IM25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLMP QTEFPMRGNL PKREPEMQKK WEEMDIYRKV QERTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KILKDIIVRY KSMSGFCAPY VPGWDTHGLP
110 120 130 140 150
IETALTKQGV DRKSMSVAEF RKLCEQYAYE QIDNQRQQFK RLGVRGDWDN
160 170 180 190 200
PYITLKPEYE AQQIKVFGEM AKKGLIYKGL KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
KDKRSPSIYV AFPVKDGKGV LQGDERIVIW TTTPWTIPAN LAIAVHPDLD
260 270 280 290 300
YYIVEANGQK YVVAAALAES VAKEVGWEAW SVVKTVKGKE LEYVVAKHPF
310 320 330 340 350
YERDSLVVCG EHVTTDAGTG CVHTAPGHGE DDFIVGQKYG LPVLCPVDER
360 370 380 390 400
GYMTEEAPGF AGMFYDEANK AITQKLEEVG ALLKLSFITH SYPHDWRTKQ
410 420 430 440 450
PTIFRATTQW FASIDKIRDQ LLDAIKETKW VPEWGEIRIH NMVRDRGDWC
460 470 480 490 500
ISRQRAWGVP IPVFYGENGE PIITDETIEH VSNLFRQYGS NVWFEREAKD
510 520 530 540 550
LLPEGFTHPS SPNGLFTKET DIMDVWFDSG SSHQAVLVER DDLERPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSLGN
610 620 630 640 650
VVVPAKVMEQ LGADILRLWV ASVDYQADVR ISDNILKQVS EVYRKIRNTF
660 670 680 690 700
RFMLGNLFDF DPNQNAVPVG ELGELDRYML AKLNKLIAKV KKAYDSYDFA
710 720 730 740 750
AVYHEMNHFC TVELSAFYLD MAKDILYIEA ADCRARRAVQ TVLYETVVAL
760 770 780 790 800
AKLIAPILPH TADEVWEHIP NRKEQVESVQ LTDMPEPMAI DGEEALLAKW
810 820 830 840 850
DAFMDVRDDI LKALENARNE KVIGKSLTAS VTVYPKDEVR ALLASINEDL
860 870 880 890 900
RQLLIVSAFS VADESYDAAP AEAERLNHVA VIVRPAEGET CERCWTVTPD
910 920
VGRDESHPTL CPRCAHIVNE HYSA
Length:924
Mass (Da):104,675
Last modified:May 1, 2007 - v1
Checksum:i1E5311B4A319A8AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA Translation: ABO66379.1
RefSeqiWP_011887108.1, NC_009328.1

Genome annotation databases

EnsemblBacteriaiABO66379; ABO66379; GTNG_1001
GeneIDi31760264
KEGGigtn:GTNG_1001

Similar proteinsi

Entry informationi

Entry nameiSYI_GEOTN
AccessioniPrimary (citable) accession number: A4IM25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health