A4IL88 (PRPE_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	GTNG_0712

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297704

Sequences

	Sequence		Length	Mass (Da)
	A4IL88 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: B075BA5C62B354B7	FASTA	245	27,791
	10 20 30 40 50 60 MHIDIIGDIH GCYREFTALT EKLGYVWDKG IPIHPDGRKL GFVGDLTDRG PESLKMIDIV 70 80 90 100 110 120 CALVDRKLAH YVPGNHCNKL YRFFLGRNVQ VTHGLETTVA EYRALPPSEQ EMIRRKFIRL 130 140 150 160 170 180 YEGAPLYAVL DEGRLVIAHA GIRHDYIGRM DKKVKTFVLY GDITGETNPD GTPVRRDWAK 190 200 210 220 230 240 RYRGDAWIVY GHTPVEKPRF VGRTVNIDTG CVFGGALTAL RYPEMTTVSV PSSMPHVPEK FRTFS

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO66092.1.
RefSeq	YP_001124837.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IL88.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IL88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4965655.
GenomeReviews	Gene locus GTNG_0712 in contig CP000557_GR.
KEGG	gtn:GTNG_0712.
NMPDR	fig|420246.5.peg.691.
PATRIC	21977731. VBIGeoThe136879_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HBG724457.
OMA	YVPGNHC.
PhylomeDB	A4IL88.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	GTHE420246:GTNG_0712-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_GEOTN

Accession

Primary (citable) accession number: A4IL88

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program