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A4IJS8 (DDL_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:GTNG_0198
OrganismGeobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]
Taxonomic identifier420246 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030450

Regions

Domain141 – 346206ATP-grasp
Nucleotide binding174 – 22956ATP By similarity

Sites

Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 2 By similarity
Metal binding3151Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4IJS8 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 43100CE88830A6A8

FASTA36440,299
        10         20         30         40         50         60 
MKTRVGVLYG GKSPEHQVSL STARAVMNAI DPHKFDVIPI YITPEGQWIK GKRLTGTVEE 

        70         80         90        100        110        120 
VKQLQFTSTA TAMIPVSLNQ VPASGSATES DEETIDVIFP LLHGPNGEDG TVQGLLEMLN 

       130        140        150        160        170        180 
LPYVGNGVLA SAVGMDKVVM KNLFAQAGLR QAKYVSVTKY EWKKGGEAVY DRIEQELGYP 

       190        200        210        220        230        240 
CFVKPANAGS SVGISKCKQR DDLKTAFAEA FKYDRKIIIE ESIVGREIEI GVIGNDEPIC 

       250        260        270        280        290        300 
SVAGEIVPKK EFYDYEAKYE DGQTELIIPA NVTEEQYETI KSMAIAAFKA LDLSGLARVD 

       310        320        330        340        350        360 
FFLAEDGTVY INEVNTMPGF TPYSMFPLLW QHSGVPYSEL IERLIALALE RHQEKQMITY 


TFEK 

« Hide

References

[1]"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NG80-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000557 Genomic DNA. Translation: ABO65582.1.
RefSeqYP_001124327.1. NC_009328.1.

3D structure databases

ProteinModelPortalA4IJS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420246.GTNG_0198.

Proteomic databases

PRIDEA4IJS8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO65582; ABO65582; GTNG_0198.
GeneID4965051.
KEGGgtn:GTNG_0198.
PATRIC21976571. VBIGeoThe136879_0211.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycGTHE420246:GIXT-230-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_GEOTN
AccessionPrimary (citable) accession number: A4IJS8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways