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A4IJP9 (ROCA_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Last modified August 10, 2010. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
Customize displayNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Names and origin

Protein namesRecommended name:
1-pyrroline-5-carboxylate dehydrogenase
Short name=P5C dehydrogenase
EC=1.5.1.12
Gene names
Name:rocA
Ordered Locus Names:GTNG_0169
OrganismGeobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]
Taxonomic identifier420246 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733

Sequence similarities

Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

proline biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular function1-pyrroline-5-carboxylate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5155151-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733
PRO_1000045971

Sites

Active site2861 By similarity
Active site3201 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4IJP9-1 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 07C58FFA56B656FD

FASTA51556,574
        10         20         30         40         50         60 
MVQPYKHEPF TDFTVDANRQ AFLAALEKVE AELGREYPLI IGGERVMTED KITSVNPANK 

        70         80         90        100        110        120 
AEVIGRVAKA NKELAERAMK TADEAFRTWS RTSPEARADI LFRAAAIVRR RKHEFSAWLV 

       130        140        150        160        170        180 
KEAGKPWREA DADTAEAIDF MEYYGRQMLK LKDGIPVESR PGETNRFFYI PLGVGVVISP 

       190        200        210        220        230        240 
WNFPFAIMAG TTVAALVTGN TVLLKPASAT PVVAYKFAEV LEEAGLPAGV LNYIPGSGAE 

       250        260        270        280        290        300 
VGDYLVEHPR TRFISFTGSR DVGIRIYERA AKVQPGQIWL KRVIAEMGGK DAIVVDKEAD 

       310        320        330        340        350        360 
LELAAQSIVA SAFGFSGQKC SACSRAIIVE DVYDQVLNRV VELTKQLNVG DPAEQATFMG 

       370        380        390        400        410        420 
PVIDQGAYNK IMEYIEIGKQ EGRLMTGGEG DDSKGFFIQP TVFADVDPNA RIMQEEIFGP 

       430        440        450        460        470        480 
VVAFAKARDF DHALEIANNT QYGLTGAVIS RNRANLEKAR HEFHVGNLYF NRGCTGAIVG 

       490        500        510 
YQPFGGFNMS GTDSKAGGPD YLILHMQAKT VSEMF

« Hide

References

[1]"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000557 Genomic DNA. Translation: ABO65553.1.
RefSeqYP_001124298.1.

3D structure databases

ProteinModelPortalA4IJP9.
SMRA4IJP9. Positions 2-515.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4IJP9.

Genome annotation databases

GeneID4965022.
GenomeReviewsGene locus GTNG_0169 in contig CP000557_GR.
KEGGgtn:GTNG_0169.
NMPDRfig|420246.5.peg.171.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHBG752218.
OMATPYKHEP.
PhylomeDBA4IJP9.
ProtClustDBPRK03137.

Enzyme and pathway databases

BioCycGTHE420246:GTNG_0169-MONOMER.

Family and domain databases

HAMAPMF_00733. RocA.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
IPR005932. d-1-pyrroline-5-COlate_DH-2.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameROCA_GEOTN
AccessionPrimary (citable) accession number: A4IJP9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: August 10, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents