Reviewed, UniProtKB/Swiss-Prot A4IJP9 (ROCA_GEOTN)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	GTNG_0169

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_1000045971
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IJP9-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 07C58FFA56B656FD	FASTA	515	56,574
	10 20 30 40 50 60 MVQPYKHEPF TDFTVDANRQ AFLAALEKVE AELGREYPLI IGGERVMTED KITSVNPANK 70 80 90 100 110 120 AEVIGRVAKA NKELAERAMK TADEAFRTWS RTSPEARADI LFRAAAIVRR RKHEFSAWLV 130 140 150 160 170 180 KEAGKPWREA DADTAEAIDF MEYYGRQMLK LKDGIPVESR PGETNRFFYI PLGVGVVISP 190 200 210 220 230 240 WNFPFAIMAG TTVAALVTGN TVLLKPASAT PVVAYKFAEV LEEAGLPAGV LNYIPGSGAE 250 260 270 280 290 300 VGDYLVEHPR TRFISFTGSR DVGIRIYERA AKVQPGQIWL KRVIAEMGGK DAIVVDKEAD 310 320 330 340 350 360 LELAAQSIVA SAFGFSGQKC SACSRAIIVE DVYDQVLNRV VELTKQLNVG DPAEQATFMG 370 380 390 400 410 420 PVIDQGAYNK IMEYIEIGKQ EGRLMTGGEG DDSKGFFIQP TVFADVDPNA RIMQEEIFGP 430 440 450 460 470 480 VVAFAKARDF DHALEIANNT QYGLTGAVIS RNRANLEKAR HEFHVGNLYF NRGCTGAIVG 490 500 510 YQPFGGFNMS GTDSKAGGPD YLILHMQAKT VSEMF

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000557 Genomic DNA. Translation: ABO65553.1.
RefSeq	YP_001124298.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4IJP9.
Genome annotation databases
GeneID	4965022.
GenomeReviews	Gene locus GTNG_0169 in contig CP000557_GR.
KEGG	gtn:GTNG_0169.
NMPDR	fig|420246.5.peg.171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FSALLVK.
Family and domain databases
HAMAP	MF_00733. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_GEOTN

Accession

Primary (citable) accession number: A4IJP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)