Reviewed, UniProtKB/Swiss-Prot A4IID1 (MGT4A_XENTR)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
      Short name=N-acetylglucosaminyltransferase IVa
      Short name=GlcNAc-T IVa
      Short name=GnT-IVa

Gene names

Name:

mgat4a

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A		PRO_0000311675
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 536	509	Lumenal Potential
	Coiled coil	28 – 54	27	Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4IID1-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 2F2067FE000238F6	FASTA	536	62,007
	10 20 30 40 50 60 MRLRNGTVAT ALVFITTFLS LSWYTAWQNG KEKLMAYQRE FHALKERLRI AEHRTLQRSS 70 80 90 100 110 120 ELHAILDHFR RMIKEANGSR DALNHFSDET QKLIKDLTNR KALQVPNIYY HMPHLLNHDG 130 140 150 160 170 180 SLQPAVQVGL GRTGVSVVMG IPTVKRRVKS YLKETLHSLI DKLSPEEKLD CVIIVFVGET 190 200 210 220 230 240 DLEYVNSVVA SLQKEFATEI SSGLVEVISP PATYYPDLNN LKETFGDSKE RVRWRTKQNL 250 260 270 280 290 300 DYCFLMMYAQ RKGIYYIQLE DDIVAKQNYF STIKNFALQL SSEDWMILEF SQLGFIGKMF 310 320 330 340 350 360 QAPDITLIVE FILMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKSNL RIRFRPSLFQ 370 380 390 400 410 420 HVGLHSSLAG KIQKLTDKDF LKPLLHKIHV NPPAEVSTSL KVYQGHTLEK TYLGEDFFWA 430 440 450 460 470 480 ITPVAGDYIL FKFDKPVNVE RYLFRSGNPE HPGDQLWNTT VEVLPYRKDI VELRSDTKDK 490 500 510 520 530 RLSDGFFRIG KFEDGLAEGP VNSYLNPISA LRLSVLQNSA VWVILNEIHI KRNPAD

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC135969 mRNA. Translation: AAI35970.1.
RefSeq	NP_001096384.1.
UniGene	Str.64551
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100124983.
KEGG	xtr:100124983.
Organism-specific databases
CTD	100124983.
Xenbase	XB-FEAT-968211. mgat4b.
Phylogenomic databases
eggNOG	veNOG10896.
Enzyme and pathway databases
BRENDA	2.4.1.145. 279072.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MGT4A_XENTR

Accession

Primary (citable) accession number: A4IID1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 2007
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project