A4IH24 (TPX2_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Targeting protein for Xklp2

Gene names

Name:

tpx2

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Spindle assembly factor. Required for normal assembly of mitotic spindles. Mediates the binding kif15 and aurka to spindle microtubules. Required for targeting kif15 to microtubule minus ends. Activates aurka by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation By similarity.
Subunit structure	Associates with microtubules. Interacts with aurka and plk1. Interacts with kif15 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Note: Localizes during metaphase on spindle microtubules, with a strong enrichment at spindle poles. Localizes to the minus ends of spindle pole and aster microtubules in a dynein- and dynactin-dependent manner By similarity.
Post-translational modification	Phosphorylated during mitosis. Hyperphosphorylated upon assembly of microtubules By similarity.
Sequence similarities	Belongs to the TPX2 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Targeting protein for Xklp2		PRO_0000393116
Amino acid modifications
	Modified residue	206	1	Phosphoserine; by plk1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IH24 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 8DC9E282DC4F267D	FASTA	709	81,373
	10 20 30 40 50 60 MAEAQDLYTF DAPSTFINFT AFHEDHGADS WFDKVTNAEN IPPDQRRLSE IPAVNAEQNG 70 80 90 100 110 120 MVEPEETSPS KETVSESAAH FSDGKSQARR SSRRMSRKHR QKLLVKMRET RLEKETAQSD 130 140 150 160 170 180 CPPTKKLKGS STKGARTPVI RGQPRSGHGS NTSPRPKAPL TLPSTPTVLK RKNVMVKPKS 190 200 210 220 230 240 SEEQELERMQ ELQKEMLENL KKNEHSMKAA ISGTGPSVKK SAVPITKPVD FHFKTDDRPK 250 260 270 280 290 300 RVADQPKGEE YKEVDFAAAL RKHPPSPVSK AALTVPKPFN LSKGKRKHEE ASEFVSTAEQ 310 320 330 340 350 360 VISFSKKTPA RYHLRSRQRE LEGPSPVKMV RPKLTNPKTP LLQTKQRLRP VMCKSAAELE 370 380 390 400 410 420 AEELERIQQY KFKAQELDSR ILEGAQVLPR KPPVKEPTKA IGFDLEIEKR IQQREKRDEG 430 440 450 460 470 480 EEEAFTFHSR PCPSKLLTEV VGVPLKKLLP VTVPHSPSFA LKNRVRLPAR EEKEEEVPAI 490 500 510 520 530 540 KATAMPHYGV PFRPKLVQQR QVEVCPFSFS DQDRERLLRK EKRMEELRKE EVHKFKAQPL 550 560 570 580 590 600 PEFGHVSLPE KRVKMPTQQE PFVLEIDKRG APRLQRWQQQ VKEEQKQQKE MAVFKARPNT 610 620 630 640 650 660 VVHQEPFLPK KESRCLTATE GFELATEKRA KERQEFEKSL AEMEAQKSLL EEETRQRQEE 670 680 690 700 EEREEISHLR KELVHKAQPI RKYKAVDVKA SDTPLTIPES PNFSDRFKC

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC135342 mRNA. Translation: AAI35343.1.
RefSeq	NP_001096233.1. NM_001102763.1.
UniGene	Str.45236.
3D structure databases
ProteinModelPortal	A4IH24.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IH24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100124787.
KEGG	xtr:100124787.
Organism-specific databases
CTD	22974.
Xenbase	XB-GENE-5810264. tpx2.
Phylogenomic databases
eggNOG	veNOG13863.
GeneTree	ENSGT00390000009842.
HOVERGEN	HBG057334.
Gene expression databases
Bgee	A4IH24.
Family and domain databases
InterPro	IPR015128. Aurora-A-bd. IPR022021. TPX2_importin. IPR009675. Xklp2_targeting_prot. [Graphical view]
Pfam	PF09041. Aurora-A_bind. 1 hit. PF06886. TPX2. 1 hit. PF12214. TPX2_importin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPX2_XENTR

Accession

Primary (citable) accession number: A4IH24

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	May 1, 2007
Last modified:	September 21, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program