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Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

  1. calcium-dependent phospholipase A2 activity Source: Ensembl
  2. calcium-dependent phospholipid binding Source: UniProtKB
  3. calcium ion binding Source: UniProtKB
  4. lysophospholipase activity Source: UniProtKB-EC
  5. phospholipase A2 activity Source: UniProtKB

GO - Biological processi

  1. arachidonic acid secretion Source: Ensembl
  2. cellular response to antibiotic Source: Ensembl
  3. icosanoid biosynthetic process Source: Ensembl
  4. phospholipid catabolic process Source: InterPro
  5. regulation of cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_271622. Arachidonic acid metabolism.
REACT_272771. Acyl chain remodelling of PE.
REACT_273525. ADP signalling through P2Y purinoceptor 1.
REACT_289244. Acyl chain remodelling of PS.
REACT_290641. Acyl chain remodelling of PC.
REACT_301543. Synthesis of PA.
REACT_315766. Acyl chain remodelling of PG.
REACT_320160. Acyl chain remodeling of CL.
REACT_321682. Platelet sensitization by LDL.
REACT_323712. Hydrolysis of LPC.
REACT_325612. Acyl chain remodelling of PI.
REACT_343292. phospho-PLA2 pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 16

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum Source: Ensembl
  4. Golgi apparatus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Cytosolic phospholipase A2PRO_0000345133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphothreonineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity
Modified residuei727 – 7271PhosphoserineBy similarity
Modified residuei729 – 7291PhosphoserineBy similarity

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-727.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Detected in granulosa cells after stimulation with chorionic gonadotropin (at protein level).1 Publication

Developmental stagei

Highly expressed in granulosa cells of ovulatory follicles. Detected at low levels in granulosa cells during the rest of the estrous cycle.

Interactioni

Subunit structurei

Interacts with KAT5.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017685.

Structurei

3D structure databases

SMRiA4IFJ5. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 740601PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingBy similarityAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiA4IFJ5.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4IFJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHHYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI
60 70 80 90 100
SSTPDSRKRT RHFNNDINPV WNETFEFILD PNQENILEIT LMDANYVMDE
110 120 130 140 150
TLGTTTFPIS SMKVGEKKQV PFIFNQVTEM ILEMSLEVCS SPDLRFSMAL
160 170 180 190 200
CDQEKAFRQQ RKENIKENMK KLLGPKNSEG LHSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INKELMKNVS HNPLLLLTPQ KIKRYVESLW RKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMNT TLSSLKEKVN TGQCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PFEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QSKGSTMEEE LENITAKHIV SNDSSDSDDE SQGPKGTEHE
460 470 480 490 500
EAEREYQNDN QASWVQRMLM ALVSDSALFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPMSPLRDF TMQESLDEDE LDAAVADPDE FEQIYEPLDV KSKKIHVVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPY VFDREGLKEC YVFKPKNPDV EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGVPRETN EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMYFN
710 720 730 740
TLNNIDVIKN AIVESIEYRR QNPSRCSVSL SSVEARRFFN KEFLSKPTA
Length:749
Mass (Da):85,350
Last modified:April 30, 2007 - v1
Checksum:iC44BF7E4C40959BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti581 – 5811S → G in AAR17479 (PubMed:16510840).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363688 mRNA. Translation: AAR17479.1.
BC134610 mRNA. Translation: AAI34611.1.
RefSeqiNP_001069332.1. NM_001075864.1.
XP_010811865.1. XM_010813563.1.
XP_010821547.1. XM_010823245.1.
UniGeneiBt.69446.

Genome annotation databases

EnsembliENSBTAT00000017685; ENSBTAP00000017685; ENSBTAG00000013298.
GeneIDi525072.
KEGGibta:525072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363688 mRNA. Translation: AAR17479.1.
BC134610 mRNA. Translation: AAI34611.1.
RefSeqiNP_001069332.1. NM_001075864.1.
XP_010811865.1. XM_010813563.1.
XP_010821547.1. XM_010823245.1.
UniGeneiBt.69446.

3D structure databases

SMRiA4IFJ5. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017685.

Chemistry

BindingDBiA4IFJ5.
ChEMBLiCHEMBL2304401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017685; ENSBTAP00000017685; ENSBTAG00000013298.
GeneIDi525072.
KEGGibta:525072.

Organism-specific databases

CTDi5321.

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiA4IFJ5.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
TreeFamiTF325228.

Enzyme and pathway databases

ReactomeiREACT_271622. Arachidonic acid metabolism.
REACT_272771. Acyl chain remodelling of PE.
REACT_273525. ADP signalling through P2Y purinoceptor 1.
REACT_289244. Acyl chain remodelling of PS.
REACT_290641. Acyl chain remodelling of PC.
REACT_301543. Synthesis of PA.
REACT_315766. Acyl chain remodelling of PG.
REACT_320160. Acyl chain remodeling of CL.
REACT_321682. Platelet sensitization by LDL.
REACT_323712. Hydrolysis of LPC.
REACT_325612. Acyl chain remodelling of PI.
REACT_343292. phospho-PLA2 pathway.

Miscellaneous databases

NextBioi20874091.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulosa cells of ovulatory follicles."
    Diouf M.N., Sayasith K., Lefebvre R., Silversides D.W., Sirois J., Lussier J.G.
    Biol. Reprod. 74:1096-1103(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Tissue: Follicular cell.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiPA24A_BOVIN
AccessioniPrimary (citable) accession number: A4IFJ5
Secondary accession number(s): Q1XD55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 2008
Last sequence update: April 30, 2007
Last modified: March 31, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.