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Protein

Cardiac phospholamban

Gene

PLN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:PLN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicSequence analysisAdd
BLAST
Transmembranei31 – 5121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Cardiac phospholambanPRO_0000353186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei16 – 161Phosphoserine; by PKA and DMPKBy similarity
Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

Post-translational modificationi

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA4IFH6.

Interactioni

Subunit structurei

Homopentamer. Interacts with HAX1 and ATP2A2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017182.

Structurei

3D structure databases

ProteinModelPortaliA4IFH6.
SMRiA4IFH6. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
HOGENOMiHOG000115660.
HOVERGENiHBG108280.
InParanoidiA4IFH6.
KOiK05852.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

A4IFH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLISI CLLLICIIVM

LL
Length:52
Mass (Da):6,054
Last modified:May 1, 2007 - v1
Checksum:i076361D9ADDC87D3
GO

Mass spectrometryi

Molecular mass is 6122.7 Da from positions 1 - 52. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134584 mRNA. Translation: AAI34585.1.
RefSeqiNP_001096789.1. NM_001103319.1.
UniGeneiBt.43673.

Genome annotation databases

GeneIDi100125240.
KEGGibta:100125240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134584 mRNA. Translation: AAI34585.1.
RefSeqiNP_001096789.1. NM_001103319.1.
UniGeneiBt.43673.

3D structure databases

ProteinModelPortaliA4IFH6.
SMRiA4IFH6. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017182.

Proteomic databases

PaxDbiA4IFH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100125240.
KEGGibta:100125240.

Organism-specific databases

CTDi5350.

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
HOGENOMiHOG000115660.
HOVERGENiHBG108280.
InParanoidiA4IFH6.
KOiK05852.

Miscellaneous databases

NextBioi20788785.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.
  2. "Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteins."
    Carroll J., Fearnley I.M., Walker J.E.
    Proc. Natl. Acad. Sci. U.S.A. 103:16170-16175(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, ACETYLATION AT MET-1, MASS SPECTROMETRY.

Entry informationi

Entry nameiPPLA_BOVIN
AccessioniPrimary (citable) accession number: A4IFH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: May 1, 2007
Last modified: April 13, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.