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Protein

Alanine aminotransferase 1

Gene

GPT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).By similarity

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. L-alanine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_217400. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1 (EC:2.6.1.2)
Short name:
ALT1
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name:
GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene namesi
Name:GPT
Synonyms:GPT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 14

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 496495Alanine aminotransferase 1PRO_0000328385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine
Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiA4IFH5.

Expressioni

Gene expression databases

ExpressionAtlasiA4IFH5. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA4IFH5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
HOVERGENiHBG026148.
InParanoidiA4IFH5.
KOiK00814.
OMAiFHAKFTL.
OrthoDBiEOG76HQ18.
TreeFamiTF300839.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4IFH5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRAGEHSQ EAANGLKEKV LTLDSMNPYV RRVEYAVRGP IVQRALELEQ
60 70 80 90 100
ELRQGVKKPF TEVIRANIGD AQAMGQIPIT FPRQVLALCV HPDLLNSPDF
110 120 130 140 150
PDDAKRRAER ILQACGGHSL GAYSISAGVQ MIREDVARYI ERRDGGIPAD
160 170 180 190 200
PNNIFLSTGA SDAIVTVLKL LVTGEGRTRT GVLIPIPQYP LYSAALAEFN
210 220 230 240 250
AVQVDYYLDE ERAWALDVAE LRRALRQARD HCRPRALCVI NPGNPTGQVQ
260 270 280 290 300
TRECIEDVIR FAYEEKLFLL ADEVYQDNVY AESSQFHSFK KVLTEMGPPY
310 320 330 340 350
AAQQELASFH SISKGYMGEC GFRGGYVEVV NMDAAVKQQM QKLRSVRLCP
360 370 380 390 400
PTPGQVLLDV AVSPPAPSDP SFPRFQAERR AVLAELAAKA KLTEQVFNEA
410 420 430 440 450
PGIRCNPVQG AMYSFPRVQL PPRAVQRAQE LGLAPDMFFC LRLLEETGIC
460 470 480 490
VVPGSGFGQR EGTYHFRMTI LPPMEKLRPL LEKLSQFHAK FTREYS
Length:496
Mass (Da):55,275
Last modified:May 1, 2007 - v1
Checksum:iEA136706AA816ED0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134583 mRNA. Translation: AAI34584.1.
RefSeqiNP_001077209.1. NM_001083740.2.
UniGeneiBt.9623.

Genome annotation databases

EnsembliENSBTAT00000010309; ENSBTAP00000010309; ENSBTAG00000007835.
GeneIDi539188.
KEGGibta:539188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134583 mRNA. Translation: AAI34584.1.
RefSeqiNP_001077209.1. NM_001083740.2.
UniGeneiBt.9623.

3D structure databases

ProteinModelPortaliA4IFH5.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA4IFH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010309; ENSBTAP00000010309; ENSBTAG00000007835.
GeneIDi539188.
KEGGibta:539188.

Organism-specific databases

CTDi2875.

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
HOVERGENiHBG026148.
InParanoidiA4IFH5.
KOiK00814.
OMAiFHAKFTL.
OrthoDBiEOG76HQ18.
TreeFamiTF300839.

Enzyme and pathway databases

UniPathwayiUPA00528; UER00586.
ReactomeiREACT_217400. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi20877836.

Gene expression databases

ExpressionAtlasiA4IFH5. baseline and differential.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiALAT1_BOVIN
AccessioniPrimary (citable) accession number: A4IFH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 1, 2007
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.