Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A4IFH5 (ALAT1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alanine aminotransferase 1

Short name=ALT1
EC=2.6.1.2
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name=GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene names
Name:GPT
Synonyms:GPT1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles By similarity.

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionAminotransferase
Transferase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-alanine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-alanine:2-oxoglutarate aminotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 496495Alanine aminotransferase 1
PRO_0000328385

Amino acid modifications

Modified residue21N-acetylalanine
Modified residue3141N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
A4IFH5 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: EA136706AA816ED0

FASTA49655,275
        10         20         30         40         50         60 
MALRAGEHSQ EAANGLKEKV LTLDSMNPYV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF 

        70         80         90        100        110        120 
TEVIRANIGD AQAMGQIPIT FPRQVLALCV HPDLLNSPDF PDDAKRRAER ILQACGGHSL 

       130        140        150        160        170        180 
GAYSISAGVQ MIREDVARYI ERRDGGIPAD PNNIFLSTGA SDAIVTVLKL LVTGEGRTRT 

       190        200        210        220        230        240 
GVLIPIPQYP LYSAALAEFN AVQVDYYLDE ERAWALDVAE LRRALRQARD HCRPRALCVI 

       250        260        270        280        290        300 
NPGNPTGQVQ TRECIEDVIR FAYEEKLFLL ADEVYQDNVY AESSQFHSFK KVLTEMGPPY 

       310        320        330        340        350        360 
AAQQELASFH SISKGYMGEC GFRGGYVEVV NMDAAVKQQM QKLRSVRLCP PTPGQVLLDV 

       370        380        390        400        410        420 
AVSPPAPSDP SFPRFQAERR AVLAELAAKA KLTEQVFNEA PGIRCNPVQG AMYSFPRVQL 

       430        440        450        460        470        480 
PPRAVQRAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPMEKLRPL 

       490 
LEKLSQFHAK FTREYS 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Hypothalamus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC134583 mRNA. Translation: AAI34584.1.
RefSeqNP_001077209.1. NM_001083740.2.
UniGeneBt.9623.

3D structure databases

ProteinModelPortalA4IFH5.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEA4IFH5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000010309; ENSBTAP00000010309; ENSBTAG00000007835.
GeneID539188.
KEGGbta:539188.

Organism-specific databases

CTD2875.

Phylogenomic databases

eggNOGCOG0436.
GeneTreeENSGT00650000093331.
HOGENOMHOG000215020.
HOVERGENHBG026148.
InParanoidA4IFH5.
KOK00814.
OMALKLMSVR.
OrthoDBEOG76HQ18.
TreeFamTF300839.

Enzyme and pathway databases

UniPathwayUPA00528; UER00586.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Other

NextBio20877836.

Entry information

Entry nameALAT1_BOVIN
AccessionPrimary (citable) accession number: A4IFH5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways