A4IFH5 (ALAT1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine aminotransferase 1
Short name=ALT1
EC=2.6.1.2

Alternative name(s):
Glutamate pyruvate transaminase 1
Short name=GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1

Gene names

Name:	GPT
Synonyms:	GPT1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles By similarity.
Catalytic activity	L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-alanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-alanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 496	495	Alanine aminotransferase 1		PRO_0000328385
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
	Modified residue	314	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IFH5 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: EA136706AA816ED0	FASTA	496	55,275
	10 20 30 40 50 60 MALRAGEHSQ EAANGLKEKV LTLDSMNPYV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF 70 80 90 100 110 120 TEVIRANIGD AQAMGQIPIT FPRQVLALCV HPDLLNSPDF PDDAKRRAER ILQACGGHSL 130 140 150 160 170 180 GAYSISAGVQ MIREDVARYI ERRDGGIPAD PNNIFLSTGA SDAIVTVLKL LVTGEGRTRT 190 200 210 220 230 240 GVLIPIPQYP LYSAALAEFN AVQVDYYLDE ERAWALDVAE LRRALRQARD HCRPRALCVI 250 260 270 280 290 300 NPGNPTGQVQ TRECIEDVIR FAYEEKLFLL ADEVYQDNVY AESSQFHSFK KVLTEMGPPY 310 320 330 340 350 360 AAQQELASFH SISKGYMGEC GFRGGYVEVV NMDAAVKQQM QKLRSVRLCP PTPGQVLLDV 370 380 390 400 410 420 AVSPPAPSDP SFPRFQAERR AVLAELAAKA KLTEQVFNEA PGIRCNPVQG AMYSFPRVQL 430 440 450 460 470 480 PPRAVQRAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPMEKLRPL 490 LEKLSQFHAK FTREYS

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC134583 mRNA. Translation: AAI34584.1.
IPI	IPI00691627.
RefSeq	NP_001077209.1. NM_001083740.2.
UniGene	Bt.9623.
3D structure databases
ProteinModelPortal	A4IFH5.
ModBase	Search...
Proteomic databases
PRIDE	A4IFH5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000010309; ENSBTAP00000010309; ENSBTAG00000007835.
GeneID	539188.
KEGG	bta:539188.
Organism-specific databases
CTD	2875.
Phylogenomic databases
eggNOG	COG0436.
GeneTree	ENSGT00650000093331.
HOGENOM	HOG000215020.
HOVERGEN	HBG026148.
InParanoid	A4IFH5.
KO	K00814.
OMA	LKLMSVR.
OrthoDB	EOG41G33Z.
Enzyme and pathway databases
UniPathway	UPA00528; UER00586.
Gene expression databases
ArrayExpress	A4IFH5.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...
Other
NextBio	20877836.

Entry information

Entry name

ALAT1_BOVIN

Accession

Primary (citable) accession number: A4IFH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program