Reviewed, UniProtKB/Swiss-Prot A4IF87 (GNPAT_BOVIN)

Names and origin

Protein names

Recommended name:
    Dihydroxyacetone phosphate acyltransferase
      Short name=DHAP-AT
      Short name=DAP-AT
    EC=2.3.1.42
Alternative name(s):
    Glycerone-phosphate O-acyltransferase
    Acyl-CoA:dihydroxyacetonephosphateacyltransferase

Gene names

Name:

GNPAT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit structure	May be part of an heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT By similarity.
Subcellular location	Peroxisome membrane; Peripheral membrane protein; Matrix side By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerone-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	Dihydroxyacetone phosphate acyltransferase		PRO_0000325855
Regions
	Motif	162 – 167	6	HXXXXD motif
	Motif	678 – 680	3	Microbody targeting signal Potential
	Compositional bias	4 – 9	6	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	A4IF87-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 9D80C804CB92854F	FASTA	680	77,664
	10 20 30 40 50 60 MDHSSSSNSC FSVGSTSPGA VVLLYSKELK KWDEFEDVLE ERRHVSDLKF AMKCYTPLVY 70 80 90 100 110 120 KGITPCKPSD IKCSVLNSEE IHYVIKQLSK ESLQPVEVLR EEACEILDEM SHKLRLGAIR 130 140 150 160 170 180 FFAFALSKIF KQIFSKVRVN EEGIQKLQRA IQEHPVVLLP SHRSYIDFLM LSFLLYNYDL 190 200 210 220 230 240 PVPVIAAGMD FLGMKMVGEL LRMSGAFFMR RTFGGNKLYW AVFSEYVKTM LRNGYAPVEF 250 260 270 280 290 300 FLEGTRSRSA KTLTPKFGLL NIVMEPFFKR EVFDTYLVPI SISYDRILEE TLYAYELLGV 310 320 330 340 350 360 PKPKESTTGL LKARRILSEK FGNIHVYFGD PVSLRSLAAG RMGRSPYNLV PRYIPQKQSE 370 380 390 400 410 420 EMHTFVTEVA YKMQLLQIQN LVLSPWPLIV AVLLQNRPSI DFDALLEKTL WLKGLTQAFG 430 440 450 460 470 480 GFLTWPDNEP AEAVIQSSIL LHSNIVSLVK DRVILKMECG DSELVDGLIF QHITLLMCLA 490 500 510 520 530 540 YRNQLLNVFV RPSLVAMALQ MTPGFRKEDV YSCFRFLCSV FSDEFIFLPG NALKDFEEGC 550 560 570 580 590 600 YLLCKSEAIQ VMTRDILVTE KGNSVLEFLI GLFKPFVESY QIICKYLLNE EEDYFTEKQY 610 620 630 640 650 660 FIRVRKFTSQ LLDQGASQCY DVLSSDVQKN ALAAFVRLGV VEKKKVNNDY IFNVNEPATT 670 680 KLEEMLGCKT PVGKPATAKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC134455 mRNA. Translation: AAI34456.1.
IPI	IPI00716800.
RefSeq	NP_001096756.1.
UniGene	Bt.41956
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000010773. Bos taurus. [Contig view]
GeneID	538800.
KEGG	bta:538800.
Enzyme and pathway databases
BRENDA	2.3.1.42. 251.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GNPAT_BOVIN

Accession

Primary (citable) accession number: A4IF87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)