Reviewed, UniProtKB/Swiss-Prot A4IF62 (RPC1_BOVIN)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit RPC1
      Short name=RNA polymerase III subunit C1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase III subunit A

Gene names

Name:

POLR3A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Antiviral defense Immune response Innate immunity Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	innate immune response Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interferon-beta production Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1390	1390	DNA-directed RNA polymerase III subunit RPC1		PRO_0000330752
Regions
	Zinc finger	69 – 82	14	C3H1-type Potential
	Region	844 – 856	13	Bridging helix By similarity
Sites
	Metal binding	69	1	Zinc 1 By similarity
	Metal binding	72	1	Zinc 1 By similarity
	Metal binding	79	1	Zinc 1 By similarity
	Metal binding	82	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 2 By similarity
	Metal binding	156	1	Zinc 2 By similarity
	Metal binding	499	1	Magnesium; catalytic By similarity
	Metal binding	501	1	Magnesium; catalytic By similarity
	Metal binding	503	1	Magnesium; catalytic By similarity
Amino acid modifications
	Modified residue	445	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IF62-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: ECF9B3A1FA466284	FASTA	1,390	155,791
	10 20 30 40 50 60 MVKEQFRETD VAKKISHICF GMKSAEEMRQ QAHIQVVSKN LYSQDNNHSP LLYGVLDHRM 70 80 90 100 110 120 GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA VIGILQMICK TCCHIMLSQE 130 140 150 160 170 180 EKKQFLDYLK RPGLTYLQKR GLKKKISDKC RKKNTCHHCG AFNGTVKKCG LLKIIHEKYK 190 200 210 220 230 240 TNKKVVDPIV SSFLQSFETA IEHNKEVEPL LGKAQENLNP LVVLNLFKRI PAEDIPLLLM 250 260 270 280 290 300 NPEAGKPSDL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG 310 320 330 340 350 360 AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR 370 380 390 400 410 420 VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN INFLRKLVRN GPEVHPGANF 430 440 450 460 470 480 IQQRHTQMKR FLKYGNREKM AQELKFGDIV ERHLIDGDVV LFNRQPSLHK LSIMAHLARV 490 500 510 520 530 540 KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA 550 560 570 580 590 600 AIQDFLTGAY LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI 610 620 630 640 650 660 FSVILRPSDD NPVRANLRTK GKQYCGRGED LCVNDSYVTI QNSELMSGSM DKGTLGSGSK 670 680 690 700 710 720 NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI GDVTPGQGLL KAKYELLNAG 730 740 750 760 770 780 YKKCDEYIEA LNTGKLQQQP GCTAEETLEA LILKELSVIR DHAGSACLRE LDKSNSPLTM 790 800 810 820 830 840 ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS 850 860 870 880 890 900 GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF 910 920 930 940 950 960 IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCRSEPAL SKNELLLSAE SIMKKNEFLC 970 980 990 1000 1010 1020 CQDSFLQEIK KFIKEVSEKI KKTRDKYGIN DNGTTEPRVL YQLDRITPTQ IEKFLETCRD 1030 1040 1050 1060 1070 1080 KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKAI 1090 1100 1110 1120 1130 1140 STPIITAQLD KDDDADYARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL 1150 1160 1170 1180 1190 1200 RLEVNAETVR YSICMSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV 1210 1220 1230 1240 1250 1260 VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN TYEVEKTLGI 1270 1280 1290 1300 1310 1320 EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV LGITRFGLAK MKESVLMLAS 1330 1340 1350 1360 1370 1380 FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKANRDP SPPRRPLIFD 1390 TNEFHIPLVT

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC134422 mRNA. Translation: AAI34423.1.
IPI	IPI00701819.
RefSeq	NP_001077219.1.
UniGene	Bt.58772
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4IF62.
Genome annotation databases
Ensembl	ENSBTAT00000017638; ENSBTAP00000017638; ENSBTAG00000013259; Bos taurus. [Genome view]
GeneID	540308.
KEGG	bta:540308.
Organism-specific databases
CTD	540308.
Family and domain databases
InterPro	IPR015700. DNA-dir_RNA_pol3_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF15. RNA_pol3. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1_BOVIN

Accession

Primary (citable) accession number: A4IF62

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 1, 2007
Last modified:	October 13, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)