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Protein

DNA-directed RNA polymerase III subunit RPC1

Gene

POLR3A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1By similarity
Metal bindingi72 – 721Zinc 1By similarity
Metal bindingi79 – 791Zinc 1By similarity
Metal bindingi82 – 821Zinc 1By similarity
Metal bindingi109 – 1091Zinc 2By similarity
Metal bindingi112 – 1121Zinc 2By similarity
Metal bindingi156 – 1561Zinc 2By similarity
Metal bindingi499 – 4991Magnesium; catalyticBy similarity
Metal bindingi501 – 5011Magnesium; catalyticBy similarity
Metal bindingi503 – 5031Magnesium; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-BTA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-BTA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-BTA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC1 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C1
Alternative name(s):
DNA-directed RNA polymerase III subunit A
Gene namesi
Name:POLR3A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 28

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13901390DNA-directed RNA polymerase III subunit RPC1PRO_0000330752Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei445 – 4451N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiA4IF62.
PRIDEiA4IF62.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017638.

Structurei

3D structure databases

ProteinModelPortaliA4IF62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni844 – 85613Bridging helixBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0260. Eukaryota.
COG0086. LUCA.
GeneTreeiENSGT00730000110946.
HOGENOMiHOG000222974.
HOVERGENiHBG004339.
InParanoidiA4IF62.
KOiK03018.
OMAiGEDLCAN.
OrthoDBiEOG761BSV.
TreeFamiTF103054.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4IF62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKEQFRETD VAKKISHICF GMKSAEEMRQ QAHIQVVSKN LYSQDNNHSP
60 70 80 90 100
LLYGVLDHRM GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA
110 120 130 140 150
VIGILQMICK TCCHIMLSQE EKKQFLDYLK RPGLTYLQKR GLKKKISDKC
160 170 180 190 200
RKKNTCHHCG AFNGTVKKCG LLKIIHEKYK TNKKVVDPIV SSFLQSFETA
210 220 230 240 250
IEHNKEVEPL LGKAQENLNP LVVLNLFKRI PAEDIPLLLM NPEAGKPSDL
260 270 280 290 300
ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG
310 320 330 340 350
AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG
360 370 380 390 400
RFRGNLSGKR VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN
410 420 430 440 450
INFLRKLVRN GPEVHPGANF IQQRHTQMKR FLKYGNREKM AQELKFGDIV
460 470 480 490 500
ERHLIDGDVV LFNRQPSLHK LSIMAHLARV KPHRTFRFNE CVCTPYNADF
510 520 530 540 550
DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA AIQDFLTGAY
560 570 580 590 600
LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI
610 620 630 640 650
FSVILRPSDD NPVRANLRTK GKQYCGRGED LCVNDSYVTI QNSELMSGSM
660 670 680 690 700
DKGTLGSGSK NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI
710 720 730 740 750
GDVTPGQGLL KAKYELLNAG YKKCDEYIEA LNTGKLQQQP GCTAEETLEA
760 770 780 790 800
LILKELSVIR DHAGSACLRE LDKSNSPLTM ALCGSKGSFI NISQMIACVG
810 820 830 840 850
QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS GLTPTEFFFH
860 870 880 890 900
TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF
910 920 930 940 950
IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCRSEPAL SKNELLLSAE
960 970 980 990 1000
SIMKKNEFLC CQDSFLQEIK KFIKEVSEKI KKTRDKYGIN DNGTTEPRVL
1010 1020 1030 1040 1050
YQLDRITPTQ IEKFLETCRD KYMRAQMEPG SAVGALCAQS IGEPGTQMTL
1060 1070 1080 1090 1100
KTFHFAGVAS MNITLGVPRI KEIINASKAI STPIITAQLD KDDDADYARL
1110 1120 1130 1140 1150
VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL RLEVNAETVR
1160 1170 1180 1190 1200
YSICMSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV
1210 1220 1230 1240 1250
VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN
1260 1270 1280 1290 1300
TYEVEKTLGI EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV
1310 1320 1330 1340 1350
LGITRFGLAK MKESVLMLAS FEKTADHLFD AAYFGQKDSV CGVSECIIMG
1360 1370 1380 1390
IPMNIGTGLF KLLHKANRDP SPPRRPLIFD TNEFHIPLVT
Length:1,390
Mass (Da):155,791
Last modified:May 1, 2007 - v1
Checksum:iECF9B3A1FA466284
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134422 mRNA. Translation: AAI34423.1.
RefSeqiNP_001077219.1. NM_001083750.1.
UniGeneiBt.58772.

Genome annotation databases

EnsembliENSBTAT00000017638; ENSBTAP00000017638; ENSBTAG00000013259.
GeneIDi540308.
KEGGibta:540308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134422 mRNA. Translation: AAI34423.1.
RefSeqiNP_001077219.1. NM_001083750.1.
UniGeneiBt.58772.

3D structure databases

ProteinModelPortaliA4IF62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017638.

Proteomic databases

PaxDbiA4IF62.
PRIDEiA4IF62.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017638; ENSBTAP00000017638; ENSBTAG00000013259.
GeneIDi540308.
KEGGibta:540308.

Organism-specific databases

CTDi11128.

Phylogenomic databases

eggNOGiKOG0260. Eukaryota.
COG0086. LUCA.
GeneTreeiENSGT00730000110946.
HOGENOMiHOG000222974.
HOVERGENiHBG004339.
InParanoidiA4IF62.
KOiK03018.
OMAiGEDLCAN.
OrthoDBiEOG761BSV.
TreeFamiTF103054.

Enzyme and pathway databases

ReactomeiR-BTA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-BTA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-BTA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-BTA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiRPC1_BOVIN
AccessioniPrimary (citable) accession number: A4IF62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 1, 2007
Last modified: July 6, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.