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Protein

Lipoyl synthase, mitochondrial

Gene

LinJ19.0190

Organism
Leishmania infantum
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (LINF_190008400), Lipoyl synthase, mitochondrial (LinJ19.0190)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi127Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi132Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi138Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi159Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi163Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi166Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:LinJ19.0190, LinJ_19_0350
OrganismiLeishmania infantum
Taxonomic identifieri5671 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000008153 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiTriTrypDB:LinJ.19.0350

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982371 – 412Lipoyl synthase, mitochondrialAdd BLAST412

Proteomic databases

PRIDEiA4HY57

Interactioni

Protein-protein interaction databases

STRINGi435258.XP_001464998.1

Structurei

3D structure databases

ProteinModelPortaliA4HY57
SMRiA4HY57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
HOGENOMiHOG000235998
InParanoidiA4HY57
KOiK03644
OMAiPYCDIDF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A4HY57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCLTAAPSRV SPVAAAAAAA ADVAGSSEST VNLMADVDKK DPQYKQIFLE
60 70 80 90 100
RFRKKLQSDK TGMNDLESFV ELPEGVAPSA ASIGPIKRGS EPLPPWLKLK
110 120 130 140 150
VPKGMTHRPR FNRIRRSMRE KNLSTVCEEA KCPNIGECWG GSDDEGTATA
160 170 180 190 200
TIMVMGSHCT RGCRFCSVLT SRRPPPLDPE EPEKVAAAVH EMGVDYIVMT
210 220 230 240 250
MVDRDDLPDG GASHVCRCIH TIKEKNPALM LEALVGDFHG DLKLVEQLAV
260 270 280 290 300
TPLSVYAHNI ECVERITPRV RDRRASYKQS LQTLEHVTKS TNGKMLTKSS
310 320 330 340 350
IMLGLGEEEK EVRQTLRDLR TAGVSAVTLG QYLQPSRTRL KVSRYAHPKE
360 370 380 390 400
FEMWEKEAMD MGFLYCASGP MVRSSYRAGE YYIKSILKQR QSAEGGKATA
410
AATAANAGAA IA
Length:412
Mass (Da):45,132
Last modified:May 1, 2007 - v1
Checksum:iCC21574ED808D2C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796451 Genomic DNA Translation: CAM67240.1
RefSeqiXP_001464998.1, XM_001464961.1

Genome annotation databases

EnsemblProtistsiCAM67240; CAM67240; LINJ_19_0350
GeneDBiLinJ.19.0350:pep
GeneIDi5068408
KEGGilif:LINJ_19_0350

Similar proteinsi

Entry informationi

Entry nameiLIPA_LEIIN
AccessioniPrimary (citable) accession number: A4HY57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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