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A4HSF7

- A4HSF7_LEIIN

UniProt

A4HSF7 - A4HSF7_LEIIN

Protein
Submitted name:

Trypanothione reductase

Gene

TRYR

Organism
Leishmania infantum
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101FADImported
    Binding sitei51 – 511FADImported
    Binding sitei56 – 561FADImported
    Binding sitei60 – 601FADImported
    Binding sitei127 – 1271FAD; via amide nitrogen and carbonyl oxygenImported
    Binding sitei159 – 1591FAD; via carbonyl oxygenImported
    Binding sitei228 – 2281NADPImported
    Binding sitei228 – 2281Sulfate 3
    Binding sitei228 – 2281Sulfate 4
    Binding sitei327 – 3271FADImported
    Binding sitei333 – 3331NADP; via carbonyl oxygenImported
    Binding sitei365 – 3651NADP; via carbonyl oxygenImported
    Binding sitei461 – 4611FAD; via carbonyl oxygenImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 152FADImported
    Nucleotide bindingi34 – 352FADImported
    Nucleotide bindingi46 – 472FADImported
    Nucleotide bindingi196 – 2027NADPImported
    Nucleotide bindingi221 – 2222NADPImported
    Nucleotide bindingi284 – 2863NADPImported
    Nucleotide bindingi333 – 3353FADImported

    GO - Molecular functioni

    1. disulfide oxidoreductase activity Source: InterPro
    2. flavin adenine dinucleotide binding Source: InterPro
    3. trypanothione-disulfide reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Ligandi

    FADUniRule annotationImported, Flavoprotein, NADPImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Trypanothione reductaseImported (EC:1.8.1.12Imported)
    Gene namesi
    Name:TRYRImported
    ORF Names:LINJ_05_0350Imported
    OrganismiLeishmania infantumImported
    Taxonomic identifieri5671 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
    ProteomesiUP000008153: Chromosome 5

    Interactioni

    Protein-protein interaction databases

    STRINGi5671.LinJ05.0350.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JK6X-ray2.95A/B1-491[»]
    2W0HX-ray3.00A/B1-491[»]
    2X50X-ray3.30A/B1-490[»]
    2YAUX-ray3.50A/B1-491[»]
    4ADWX-ray3.61A/B1-491[»]
    4APNX-ray3.20A/B1-491[»]
    ProteinModelPortaliA4HSF7.
    SMRiA4HSF7. Positions 2-486.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA4HSF7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni221 – 2222Sulfate 3 binding
    Regioni221 – 2222Sulfate 4 binding
    Regioni284 – 2863Sulfate 2 binding
    Regioni284 – 2863Sulfate 3 binding

    Sequence similaritiesi

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.UniRule annotation

    Keywords - Domaini

    Redox-active centerUniRule annotation

    Phylogenomic databases

    eggNOGiCOG1249.
    KOiK04283.
    OMAiDAKNYGW.

    Family and domain databases

    Gene3Di3.30.390.30. 1 hit.
    InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    IPR001864. Trypnth_redctse.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    PR00470. TRYPANRDTASE.
    SUPFAMiSSF55424. SSF55424. 1 hit.
    TIGRFAMsiTIGR01423. trypano_reduc. 1 hit.
    PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4HSF7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRAYDLVVL GAGSGGLEAG WNAAVTHKKK VAVVDVQATH GPPLFAALGG    50
    TCVNVGCVPK KLMVTGAQYM DLIRESGGFG WEMDRESLCP NWKTLIAAKN 100
    KVVNSINESY KSMFADTEGL SFHMGFGALQ DAHTVVVRKS EDPHSDVLET 150
    LDTEYILIAT GSWPTRLGVP GDEFCITSNE AFYLEDAPKR MLCVGGGYIA 200
    VEFAGIFNGY KPCGGYVDLC YRGDLILRGF DTEVRKSLTK QLGANGIRVR 250
    TNLNPTKITK NEDGSNHVHF NDGTEEDYDQ VMLAIGRVPR SQALQLDKAG 300
    VRTGKNGAVQ VDAYSKTSVD NIYAIGDVTN RVMLTPVAIN EGAAFVETVF 350
    GGKPRATDHT KVACAVFSIP PIGTCGMTEE EAAKNYETVA VYASSFTPLM 400
    HNISGSKHKE FMIRIITNES NGEVLGVHML GDSAPEIIQS VGICMKMGAK 450
    ISDFHSTIGV HPTSAEELCS MRTPAYFYES GKRVEKLSSN L 491
    Length:491
    Mass (Da):53,026
    Last modified:May 1, 2007 - v1
    Checksum:i1E41FDC657DDCF67
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796437 Genomic DNA. Translation: CAM65344.1.
    RefSeqiXP_001462998.1. XM_001462961.1.

    Genome annotation databases

    GeneIDi5066544.
    KEGGilif:LINJ_05_0350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796437 Genomic DNA. Translation: CAM65344.1 .
    RefSeqi XP_001462998.1. XM_001462961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JK6 X-ray 2.95 A/B 1-491 [» ]
    2W0H X-ray 3.00 A/B 1-491 [» ]
    2X50 X-ray 3.30 A/B 1-490 [» ]
    2YAU X-ray 3.50 A/B 1-491 [» ]
    4ADW X-ray 3.61 A/B 1-491 [» ]
    4APN X-ray 3.20 A/B 1-491 [» ]
    ProteinModelPortali A4HSF7.
    SMRi A4HSF7. Positions 2-486.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5671.LinJ05.0350.

    Chemistry

    ChEMBLi CHEMBL1944501.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5066544.
    KEGGi lif:LINJ_05_0350.

    Phylogenomic databases

    eggNOGi COG1249.
    KOi K04283.
    OMAi DAKNYGW.

    Miscellaneous databases

    EvolutionaryTracei A4HSF7.

    Family and domain databases

    Gene3Di 3.30.390.30. 1 hit.
    InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    IPR001864. Trypnth_redctse.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    PR00470. TRYPANRDTASE.
    SUPFAMi SSF55424. SSF55424. 1 hit.
    TIGRFAMsi TIGR01423. trypano_reduc. 1 hit.
    PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JPCM5Imported.
    2. "Molecular basis of antimony treatment in leishmaniasis."
      Baiocco P., Colotti G., Franceschini S., Ilari A.
      J. Med. Chem. 52:2603-2612(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) IN COMPLEX WITH FAD AND NADP.
    3. "Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leishmania infantum Proliferation."
      Baiocco P., Ilari A., Ceci P., Orsini S., Gramiccia M., Di Muccio T., Colotti G.
      ACS Med. Chem. Lett. 2:230-233(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.30 ANGSTROMS) OF 1-490 IN COMPLEX WITH FAD AND NADP.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JPCM5Imported.
    5. "A gold-containing drug against parasitic polyamine metabolism: the X-ray structure of trypanothione reductase from Leishmania infantum in complex with auranofin reveals a dual mechanism of enzyme inhibition."
      Ilari A., Baiocco P., Messori L., Fiorillo A., Boffi A., Gramiccia M., Di Muccio T., Colotti G.
      Amino Acids 42:803-811(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) IN COMPLEX WITH FAD AND NADP.
    6. "Inhibition of Leishmania infantum trypanothione reductase by azole-based compounds: a comparative analysis with its physiological substrate by X-ray crystallography."
      Baiocco P., Poce G., Alfonso S., Cocozza M., Porretta G.C., Colotti G., Biava M., Moraca F., Botta M., Yardley V., Fiorillo A., Lantella A., Malatesta F., Ilari A.
      ChemMedChem 8:1175-1183(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN COMPLEX WITH FAD AND NADP.

    Entry informationi

    Entry nameiA4HSF7_LEIIN
    AccessioniPrimary (citable) accession number: A4HSF7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 1, 2007
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3