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A4H9Z3

- LIPA_LEIBR

UniProt

A4H9Z3 - LIPA_LEIBR

Protein

Lipoyl synthase, mitochondrial

Gene

LbrM19_V2.0670

Organism
Leishmania braziliensis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi126 – 1261Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:LbrM19_V2.0670, LbrM_19_0670
    OrganismiLeishmania braziliensis
    Taxonomic identifieri5660 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmaniaLeishmania braziliensis species complex
    ProteomesiUP000007258: Chromosome 19

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 379Lipoyl synthase, mitochondrialPRO_0000398236
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliA4H9Z3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OMAiHPHIPTK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4H9Z3-1 [UniParc]FASTAAdd to Basket

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    MTDVDKKDPQ YKQIFLERFR KKLQSDKTGM SDLESFVELP EGITPVAASI    50
    GPIKRGSEPL PPWLKLNVPK GMTHRPRFNR IRRSMREKKL STVCEEAKCP 100
    NIGECWGGGE DNGAATATIM VMGSHCTRGC RFCSVLTSRR PPPLDPDEPE 150
    KVAAAVHEMG VDYIVMTMVD RDDLPDGGAS HVSRCIRTIK EQNPELMLEA 200
    LVGDFHGDLK VVEQLAATPL SVYAHNIECV ERITPRVRDH RATYKQSLQT 250
    LEHVTKWTNG KMLTKSSIML GLGEEEEEVR QTLRDLRTAG VSAVTLGQYL 300
    QPSRTRLKVS RYAHPKEFEM WEKEAMSMGF LYCASGPMVR SSYRAGEYYI 350
    KNIVKQRENA KGATTMEAVT AADAVAAGA 379
    Length:379
    Mass (Da):42,320
    Last modified:May 1, 2007 - v1
    Checksum:i6FEC94249B0FA50F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR798993 Genomic DNA. Translation: CAM38219.1.
    RefSeqiXP_001564163.2. XM_001564113.2.

    Genome annotation databases

    GeneIDi5414704.
    KEGGilbz:LBRM_19_0670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR798993 Genomic DNA. Translation: CAM38219.1 .
    RefSeqi XP_001564163.2. XM_001564113.2.

    3D structure databases

    ProteinModelPortali A4H9Z3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5414704.
    KEGGi lbz:LBRM_19_0670.

    Phylogenomic databases

    KOi K03644.
    OMAi HPHIPTK.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MHOM/BR/75/M2904.

    Entry informationi

    Entry nameiLIPA_LEIBR
    AccessioniPrimary (citable) accession number: A4H9Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3