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Protein

Probable crossover junction endonuclease EME2

Gene

EME2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Enzyme and pathway databases

ReactomeiR-HSA-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-HSA-6783310. Fanconi Anemia Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable crossover junction endonuclease EME2 (EC:3.1.22.-)
Gene namesi
Name:EME2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:27289. EME2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000197774.
PharmGKBiPA134863517.

Polymorphism and mutation databases

BioMutaiEME2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003173731 – 379Probable crossover junction endonuclease EME2Add BLAST379

Proteomic databases

PRIDEiA4GXA9.

Expressioni

Gene expression databases

BgeeiENSG00000197774.
CleanExiHS_EME2.
ExpressionAtlasiA4GXA9. baseline and differential.

Organism-specific databases

HPAiHPA045770.

Interactioni

Subunit structurei

Interacts with MUS81.1 Publication

Protein-protein interaction databases

BioGridi128252. 4 interactors.
IntActiA4GXA9. 1 interactor.
MINTiMINT-8373003.

Structurei

3D structure databases

ProteinModelPortaliA4GXA9.
SMRiA4GXA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EME1/MMS4 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063937.
HOGENOMiHOG000112363.
HOVERGENiHBG107847.
InParanoidiA4GXA9.
KOiK10883.
OMAiCECRIEP.
OrthoDBiEOG091G0CET.
TreeFamiTF325310.

Family and domain databases

InterProiIPR006166. ERCC4_domain.
IPR033310. Mms4/EME1/EME2.
[Graphical view]
PANTHERiPTHR21077. PTHR21077. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A4GXA9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARVGPGRAG VSCQGRGRGR GGSGQRRPPT WEISDSDAED SAGSEAAARA
60 70 80 90 100
RDPAGERRAA AEALRLLRPE QVLKRLAVCV DTAILEDAGA DVLMEALEAL
110 120 130 140 150
GCECRIEPQR PARSLRWTRA SPDPCPRSLP PEVWAAGEQE LLLLLEPEEF
160 170 180 190 200
LQGVATLTQI SGPTHWVPWI SPETTARPHL AVIGLDAYLW SRQHVSRGTQ
210 220 230 240 250
QPESPKVAGA EVAVSWPEVE EALVLLQLWA NLDVLLVASW QELSRHVCAV
260 270 280 290 300
TKALAQYPLK QYRESQAFSF CTAGRWAAGE PVARDGAGLQ AAWRRQIRQF
310 320 330 340 350
SRVSPAVADA VVTAFPSPRL LQQALEACST ERERMGLLAD LPVPPSEGGR
360 370
PRRVGPDLSR RICLFLTTAN PDLLLDLGS
Length:379
Mass (Da):41,178
Last modified:May 14, 2014 - v3
Checksum:i95483C4A4A1C1946
GO
Isoform 2 (identifier: A4GXA9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.
     135-190: AAGEQELLLL...AVIGLDAYLW → MPTAGLAGTG...SGEEWSPLLR

Note: No experimental confirmation available.
Show »
Length:245
Mass (Da):26,446
Checksum:iD8C3B561B6DE5CD8
GO

Sequence cautioni

The sequence AAK61292 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB84906 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0309381 – 134Missing in isoform 2. 1 PublicationAdd BLAST134
Alternative sequenceiVSP_030939135 – 190AAGEQ…DAYLW → MPTAGLAGTGVQGRWAHFWG CCGTADPTSPGGLWQRPSSG RAGPMGSGEEWSPLLR in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF452422 mRNA. Translation: ABO21766.1.
AK074080 mRNA. Translation: BAB84906.2. Different initiation.
AE006639 Genomic DNA. Translation: AAK61292.1. Sequence problems.
CCDSiCCDS58404.1. [A4GXA9-1]
RefSeqiNP_001244299.1. NM_001257370.1. [A4GXA9-1]
UniGeneiHs.7247.

Genome annotation databases

EnsembliENST00000568449; ENSP00000457353; ENSG00000197774. [A4GXA9-1]
GeneIDi197342.
KEGGihsa:197342.
UCSCiuc010brw.2. human. [A4GXA9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF452422 mRNA. Translation: ABO21766.1.
AK074080 mRNA. Translation: BAB84906.2. Different initiation.
AE006639 Genomic DNA. Translation: AAK61292.1. Sequence problems.
CCDSiCCDS58404.1. [A4GXA9-1]
RefSeqiNP_001244299.1. NM_001257370.1. [A4GXA9-1]
UniGeneiHs.7247.

3D structure databases

ProteinModelPortaliA4GXA9.
SMRiA4GXA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128252. 4 interactors.
IntActiA4GXA9. 1 interactor.
MINTiMINT-8373003.

Polymorphism and mutation databases

BioMutaiEME2.

Proteomic databases

PRIDEiA4GXA9.

Protocols and materials databases

DNASUi197342.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000568449; ENSP00000457353; ENSG00000197774. [A4GXA9-1]
GeneIDi197342.
KEGGihsa:197342.
UCSCiuc010brw.2. human. [A4GXA9-1]

Organism-specific databases

CTDi197342.
GeneCardsiEME2.
HGNCiHGNC:27289. EME2.
HPAiHPA045770.
MIMi610886. gene.
neXtProtiNX_A4GXA9.
OpenTargetsiENSG00000197774.
PharmGKBiPA134863517.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00530000063937.
HOGENOMiHOG000112363.
HOVERGENiHBG107847.
InParanoidiA4GXA9.
KOiK10883.
OMAiCECRIEP.
OrthoDBiEOG091G0CET.
TreeFamiTF325310.

Enzyme and pathway databases

ReactomeiR-HSA-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-HSA-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

ChiTaRSiEME2. human.
GenomeRNAii197342.
PROiA4GXA9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197774.
CleanExiHS_EME2.
ExpressionAtlasiA4GXA9. baseline and differential.

Family and domain databases

InterProiIPR006166. ERCC4_domain.
IPR033310. Mms4/EME1/EME2.
[Graphical view]
PANTHERiPTHR21077. PTHR21077. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEME2_HUMAN
AccessioniPrimary (citable) accession number: A4GXA9
Secondary accession number(s): Q8TEP2, Q96RY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 14, 2014
Last modified: November 2, 2016
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.