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Protein

ETS domain-containing protein Elk-1

Gene

Elk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (By similarity) (PubMed:17156131). Induces target gene transcription upon JNK-signaling pathway stimulation (PubMed:17156131).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi5 – 8682ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • cellular response to gamma radiation Source: RGD
  • cellular response to lipid Source: RGD
  • cellular response to testosterone stimulus Source: RGD
  • positive regulation of neuron death Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to fibroblast growth factor Source: UniProtKB
  • response to light stimulus Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-1Curated
Gene namesi
Name:Elk1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1598663. Elk1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • axon terminus Source: RGD
  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • mitochondrion Source: RGD
  • neuronal cell body Source: RGD
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427ETS domain-containing protein Elk-1PRO_0000433929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki229 – 229Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki248 – 248Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki253 – 253Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei323 – 3231Phosphoserine; by MAPK1By similarity
Modified residuei335 – 3351Phosphothreonine; by MAPK1By similarity
Modified residuei352 – 3521Phosphothreonine; by MAPK1By similarity
Modified residuei362 – 3621Phosphothreonine; by MAPK1By similarity
Modified residuei367 – 3671Phosphothreonine; by MAPK1By similarity
Modified residuei382 – 3821Phosphoserine; by MAPK1 and MAPK8By similarity
Modified residuei388 – 3881Phosphoserine; by MAPK1By similarity
Modified residuei416 – 4161Phosphothreonine; by MAPK1By similarity
Modified residuei421 – 4211Phosphoserine; by MAPK1By similarity

Post-translational modificationi

Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.By similarity
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-382 and Ser-388 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-382 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14 (By similarity). Upon bFGF stimulus, phosphorylated by PAK1 (PubMed:17156131).By similarity1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA4GTP4.

PTM databases

iPTMnetiA4GTP4.
PhosphoSiteiA4GTP4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010171.
GenevisibleiA4GTP4. RN.

Interactioni

Subunit structurei

Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9. Interacts with POU1F1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013522.

Structurei

3D structure databases

ProteinModelPortaliA4GTP4.
SMRiA4GTP4. Positions 5-90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni348 – 39851Sufficient for interaction with MAD2L2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.UniRule annotation
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
KOiK04375.
OMAiKSEEPNM.
OrthoDBiEOG091G0CL4.
PhylomeDBiA4GTP4.
TreeFamiTF317732.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4GTP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK
60 70 80 90 100
NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP
110 120 130 140 150
PQPEVSVTSA VAMAPATVHS GPGDNATGKP GTPKGAGMTG QGGLARSSRN
160 170 180 190 200
EYMRSGLYST FTIQSLQPQP PLHPRPASVL PNTTPAGVPA PPSGSRSTSP
210 220 230 240 250
NPLEACLEAE EAGLPLQVIL TPPEAPNQKS EELSLNPGFG RPQPPEVKVE
260 270 280 290 300
GPKEELEVTE VGGFSPEAVK AEQEVSPSEG LLARLPAILT ENTAQVCGLS
310 320 330 340 350
TSTTEITQPQ KGRKPRDLEL PLSPSLLGGQ GPERTPGSGT SSGLQAQGPA
360 370 380 390 400
LTPSLLPTHT LTPVLLTPSS LPPSIHFWST LSPIAPRSPA KLSFQFPSSG
410 420
SAQVHIPSIS VDGLSTPVVL SPGPQKP
Length:427
Mass (Da):45,272
Last modified:April 17, 2007 - v1
Checksum:iC318C5A55E4785E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF429098 mRNA. Translation: ABO27185.1.
AABR07036739 Genomic DNA. No translation available.
CH474009 Genomic DNA. Translation: EDL97728.1.
RefSeqiNP_001101529.1. NM_001108059.3.
XP_006256676.1. XM_006256614.2.
UniGeneiRn.203416.

Genome annotation databases

EnsembliENSRNOT00000013522; ENSRNOP00000013522; ENSRNOG00000010171.
GeneIDi314436.
KEGGirno:314436.
UCSCiRGD:1598663. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF429098 mRNA. Translation: ABO27185.1.
AABR07036739 Genomic DNA. No translation available.
CH474009 Genomic DNA. Translation: EDL97728.1.
RefSeqiNP_001101529.1. NM_001108059.3.
XP_006256676.1. XM_006256614.2.
UniGeneiRn.203416.

3D structure databases

ProteinModelPortaliA4GTP4.
SMRiA4GTP4. Positions 5-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013522.

PTM databases

iPTMnetiA4GTP4.
PhosphoSiteiA4GTP4.

Proteomic databases

PaxDbiA4GTP4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013522; ENSRNOP00000013522; ENSRNOG00000010171.
GeneIDi314436.
KEGGirno:314436.
UCSCiRGD:1598663. rat.

Organism-specific databases

CTDi2002.
RGDi1598663. Elk1.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
KOiK04375.
OMAiKSEEPNM.
OrthoDBiEOG091G0CL4.
PhylomeDBiA4GTP4.
TreeFamiTF317732.

Miscellaneous databases

PROiA4GTP4.

Gene expression databases

BgeeiENSRNOG00000010171.
GenevisibleiA4GTP4. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELK1_RAT
AccessioniPrimary (citable) accession number: A4GTP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.