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Protein

Nuclear-pore anchor

Gene

NUA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex. Acts as a docking site for activities required for desumoylation and mRNA export. Required for the proper expression or localization of a subset of miRNAs. Plays a role in meristematic cell division by interacting with spindle assembly checkpoint proteins.1 Publication4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear-pore anchor2 Publications
Alternative name(s):
Protein TRANSLOCATED PROMOTER REGION1 Publication
Short name:
AtTPR
Gene namesi
Name:NUA2 Publications
Synonyms:TPR1 Publication
Ordered Locus Names:At1g79280Imported
ORF Names:YUP8H12R.11, YUP8H12R.12, YUP8H12R.8, YUP8H12R.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G79280.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Early flowering under both long and short days and pleiotropic alterations in shoot development and auxin signaling. Stunted primary root development in the absence of sucrose. Accumulation of nuclear poly(A)+ RNA and high-molecular weight SUMO conjugates.1 Publication2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003959761 – 2093Nuclear-pore anchorAdd BLAST2093

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2022PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA4GSN8.
PRIDEiA4GSN8.

PTM databases

iPTMnetiA4GSN8.

Expressioni

Tissue specificityi

Ubiquitous. Highest expression in the shoot apical region.2 Publications

Inductioni

Not induced by vernalization.1 Publication

Gene expression databases

ExpressionAtlasiA4GSN8. baseline and differential.
GenevisibleiA4GSN8. AT.

Interactioni

Subunit structurei

Part of the nuclear pore complex (NPC). The NPC has an eight-fold symmetrical structure comprising a central transport channel and two rings, the cytoplasmic and nuclear rings, to which eight filaments are attached. The cytoplasmic filaments have loose ends, while the nuclear filaments are joined in a distal ring, forming a nuclear basket. NPCs are highly dynamic in configuration and composition, and can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160 subcomplex and the NUP93 subcomplex, containing approximately 30 different nucleoporin proteins. Interacts with MAD1 and (via N-terminus) with ESD4.1 Publication2 Publications

Protein-protein interaction databases

BioGridi29487. 7 interactors.
IntActiA4GSN8. 1 interactor.
STRINGi3702.AT1G79280.2.

Structurei

3D structure databases

ProteinModelPortaliA4GSN8.
SMRiA4GSN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili57 – 362Sequence analysisAdd BLAST306
Coiled coili439 – 529Sequence analysisAdd BLAST91
Coiled coili570 – 627Sequence analysisAdd BLAST58
Coiled coili688 – 1172Sequence analysisAdd BLAST485
Coiled coili1208 – 1252Sequence analysisAdd BLAST45
Coiled coili1293 – 1585Sequence analysisAdd BLAST293
Coiled coili1818 – 1849Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1372 – 1557Lys-richAdd BLAST186
Compositional biasi1824 – 1961Glu-richAdd BLAST138
Compositional biasi2036 – 2086Arg-richAdd BLAST51

Domaini

The N-terminal domain is involved in RNA and SUMO homeostasis while the C-terminal part is required for nuclear pore association.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4674. Eukaryota.
ENOG410XSA1. LUCA.
HOGENOMiHOG000254782.
InParanoidiA4GSN8.
KOiK09291.
OMAiTEANCTI.
OrthoDBiEOG093600DQ.
PhylomeDBiA4GSN8.

Family and domain databases

InterProiIPR012929. TPR/MLP1.
[Graphical view]
PfamiPF07926. TPR_MLP1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A4GSN8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLFMPDEEL ARLSSDAASV VAERADEYIR KIYAELDSVR AKADAASITA
60 70 80 90 100
EQTCSLLEQK YLSLSQDFSS LESQNAKLQS DFDDRLAELA QSQAQKHQLH
110 120 130 140 150
LQSIEKDGEV ERMSTEMSEL HKSKRQLMEL LEQKDAEISE KNSTIKSYLD
160 170 180 190 200
KIVKLTDTSS EKEARLAEAT AELARSQAMC SRLSQEKELT ERHAKWLDEE
210 220 230 240 250
LTAKVDSYAE LRRRHSDLES EMSAKLVDVE KNYIECSSSL NWHKERLREL
260 270 280 290 300
ETKIGSLQED LSSCKDAATT TEEQYTAELF TANKLVDLYK ESSEEWSRKA
310 320 330 340 350
GELEGVIKAL EARLSQVESS YKERLDKEVS TKQLLEKENG DLKQKLEKCE
360 370 380 390 400
AEIEKTRKTD ELNLIPFSNF TRRVDNSGTS NMIEESQAVI SKVPAGVSGT
410 420 430 440 450
ALAASLLRDG WSLAKIYEKY QEAVDAMRHE QLGRKEAEMI LQRVLSELEE
460 470 480 490 500
KAGFIQEERG EYERVVEAYC LVNQKLQDSV SEQSNMEKFI MELKADLRRR
510 520 530 540 550
ERENTLLQKD ISDLQKQVTI LLKECRDVQL RCGAARDDDE DDYPLLSDVE
560 570 580 590 600
MEMESEADKI ISEHLLKFKD INGLVEQNVK LRNLVRSLSE QIESRETELK
610 620 630 640 650
ETFEVDLKNK TDEASAKVAT VLKRAEEQGQ MIESLHTSVA MYKRLYEEEQ
660 670 680 690 700
KLHSSDSRSS DLSPAVVPGR KNFLHLLEDS EEATKRAQEK AFERIRILEE
710 720 730 740 750
DFAKARSEVI AIRSERDKLA MEANFAREKL EGIMKESERK REEMNSVLAR
760 770 780 790 800
NIEFSQLIID HQRKLRESSE SLHAAEEISR KLSMEVSVLK QEKELLSNAE
810 820 830 840 850
KRASDEVSAL SQRVYRLQAT LDTVQSTEEV REETRAAERR KQEEHIKQLQ
860 870 880 890 900
REWAEAKKEL QEERSNARDF TSDRNQTLNN AVMQVEEMGK ELANALKAVS
910 920 930 940 950
VAESRASVAE ARLSDLEKKI RSSDPKTLDM DSGGIVSLSD KEMSIELRTA
960 970 980 990 1000
KEEIEKLRGE VESSKSHMLQ YKSIAQVNET ALKQMESAHE NFRLEAEKRQ
1010 1020 1030 1040 1050
RSLEAELVSL RERVSELEND CIQKSEQLAT AAAGKEDALL SASAEIASLR
1060 1070 1080 1090 1100
EENLVKKSQI EAMNIQMSTL KNDLETEHEK WRVAQRNYER QVILLSETIQ
1110 1120 1130 1140 1150
ELTKTSQALA ALQEEASELR KLADARGIEN SELNAKWSEE KLMLEQQKNL
1160 1170 1180 1190 1200
AEKKYHELNE QNKLLHSRLE AKHLNSAEKN SRSGTISSGS TDSDHLEDSG
1210 1220 1230 1240 1250
LQRVVHYLRR TKEIAETEIS LMRQEKLRLQ SQLESALKMA ESARGSLTAE
1260 1270 1280 1290 1300
RASTRASLLT DDGIKSLQLQ VSEMNLLRES NMQLREENKH NFEKCQEMRE
1310 1320 1330 1340 1350
VAQKARMESE NFENLLKTKQ TELDLCMKEM EKLRMETDLH KKRVDELRET
1360 1370 1380 1390 1400
YRNIDIADYN RLKDEVRQLE EKLKAKDAHA EDCKKVLLEK QNKISLLEKE
1410 1420 1430 1440 1450
LTNCKKDLSE REKRLDDAQQ AQATMQSEFN KQKQELEKNK KIHYTLNMTK
1460 1470 1480 1490 1500
RKYEKEKDEL SKQNQSLAKQ LEEAKEEAGK RTTTDAVVEQ SVKEREEKEK
1510 1520 1530 1540 1550
RIQILDKYVH QLKDEVRKKT EDLKKKDEEL TKERSERKSV EKEVGDSLTK
1560 1570 1580 1590 1600
IKKEKTKVDE ELAKLERYQT ALTHLSEELE KLKHADGNLP EGTSAVQVLS
1610 1620 1630 1640 1650
GSILNDQAAA YVSAVEYFER VARSIASNSQ VSTKPTDMVT EPSSGIPAAE
1660 1670 1680 1690 1700
PSTMTRVPSS TPLIKSPVAT TQQLPKVASD NKEKRLISQK PSTEFRRPSG
1710 1720 1730 1740 1750
RRIVRPQLVK PEESPKVDVD MPEAEGTGDE GKQPAAHEPE SQVTTSVRPV
1760 1770 1780 1790 1800
QTLVRKRQAD SLVSEPQQDS LTQGETSSEI APPASKKAKG SESHPDTSEG
1810 1820 1830 1840 1850
ENLAKEPAID ELMDATTTTD GDNEETEAEN AEEKTEEYVE AQQDNEADEP
1860 1870 1880 1890 1900
VEESPTETET IPTEEESRDQ TEEENQEPLT DMESDKEEGE LDLDTLEDLE
1910 1920 1930 1940 1950
EGTDVASMMR SPEKEEVQPE TLATPTQSPS RMETAMEEAE TTIETPVEDD
1960 1970 1980 1990 2000
KTDEGGDAAE EAADIPNNAN DQQEAPETDI KPETSAATTS PVSTAPTTSS
2010 2020 2030 2040 2050
TLASAITSSG APETEDPKRA PSPGGGSSTI VTLADRAQMK RRERIANIVV
2060 2070 2080 2090
SRAPNPATRG ARGRTVNLRG GGRLLPRGGR APRGGRGQSP SPP
Length:2,093
Mass (Da):237,040
Last modified:April 17, 2007 - v1
Checksum:iDC03ED0639506402
GO
Isoform 2 (identifier: A4GSN8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     942-942: E → EAALVIILDVVHKIQAGFRIGSA

Show »
Length:2,115
Mass (Da):239,314
Checksum:i79025C1953C64F5F
GO
Isoform 3 (identifier: A4GSN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     787-792: SVLKQE → VYHHPLK

Show »
Length:2,094
Mass (Da):237,231
Checksum:iC0FD032EDCEF0966
GO

Sequence cautioni

The sequence AAC17058 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC17060 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC17073 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC17074 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057127787 – 792SVLKQE → VYHHPLK in isoform 3. 6
Alternative sequenceiVSP_057128942E → EAALVIILDVVHKIQAGFRI GSA in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF426860 mRNA. Translation: ABO21684.1.
AC002986 Genomic DNA. Translation: AAC17058.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17060.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17073.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17074.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36226.1.
CP002684 Genomic DNA. Translation: AEE36227.1.
CP002684 Genomic DNA. Translation: AEE36228.1.
AF083705 mRNA. Translation: AAN60264.1.
PIRiT01025.
T01026.
T01028.
T01029.
RefSeqiNP_001185435.1. NM_001198506.1. [A4GSN8-3]
NP_001185436.1. NM_001198507.1. [A4GSN8-2]
NP_178048.2. NM_106578.3. [A4GSN8-1]
UniGeneiAt.48393.

Genome annotation databases

EnsemblPlantsiAT1G79280.1; AT1G79280.1; AT1G79280. [A4GSN8-1]
AT1G79280.2; AT1G79280.2; AT1G79280. [A4GSN8-2]
AT1G79280.3; AT1G79280.3; AT1G79280. [A4GSN8-3]
GeneIDi844268.
GrameneiAT1G79280.1; AT1G79280.1; AT1G79280.
AT1G79280.2; AT1G79280.2; AT1G79280.
AT1G79280.3; AT1G79280.3; AT1G79280.
KEGGiath:AT1G79280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF426860 mRNA. Translation: ABO21684.1.
AC002986 Genomic DNA. Translation: AAC17058.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17060.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17073.1. Sequence problems.
AC002986 Genomic DNA. Translation: AAC17074.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36226.1.
CP002684 Genomic DNA. Translation: AEE36227.1.
CP002684 Genomic DNA. Translation: AEE36228.1.
AF083705 mRNA. Translation: AAN60264.1.
PIRiT01025.
T01026.
T01028.
T01029.
RefSeqiNP_001185435.1. NM_001198506.1. [A4GSN8-3]
NP_001185436.1. NM_001198507.1. [A4GSN8-2]
NP_178048.2. NM_106578.3. [A4GSN8-1]
UniGeneiAt.48393.

3D structure databases

ProteinModelPortaliA4GSN8.
SMRiA4GSN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29487. 7 interactors.
IntActiA4GSN8. 1 interactor.
STRINGi3702.AT1G79280.2.

PTM databases

iPTMnetiA4GSN8.

Proteomic databases

PaxDbiA4GSN8.
PRIDEiA4GSN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G79280.1; AT1G79280.1; AT1G79280. [A4GSN8-1]
AT1G79280.2; AT1G79280.2; AT1G79280. [A4GSN8-2]
AT1G79280.3; AT1G79280.3; AT1G79280. [A4GSN8-3]
GeneIDi844268.
GrameneiAT1G79280.1; AT1G79280.1; AT1G79280.
AT1G79280.2; AT1G79280.2; AT1G79280.
AT1G79280.3; AT1G79280.3; AT1G79280.
KEGGiath:AT1G79280.

Organism-specific databases

TAIRiAT1G79280.

Phylogenomic databases

eggNOGiKOG4674. Eukaryota.
ENOG410XSA1. LUCA.
HOGENOMiHOG000254782.
InParanoidiA4GSN8.
KOiK09291.
OMAiTEANCTI.
OrthoDBiEOG093600DQ.
PhylomeDBiA4GSN8.

Miscellaneous databases

PROiA4GSN8.

Gene expression databases

ExpressionAtlasiA4GSN8. baseline and differential.
GenevisibleiA4GSN8. AT.

Family and domain databases

InterProiIPR012929. TPR/MLP1.
[Graphical view]
PfamiPF07926. TPR_MLP1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUA_ARATH
AccessioniPrimary (citable) accession number: A4GSN8
Secondary accession number(s): F4IDK8
, F4IDK9, O64521, O64522, O64524, O64525, Q8H7F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: April 17, 2007
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.