UniProtKB - A4GCL6 (RDRP_I43A0)
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Protein
RNA-directed RNA polymerase catalytic subunit
Gene
PB1
Organism
Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Status
Functioni
RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation
Catalytic activityi
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
- viral RNA genome replication Source: InterPro
- viral transcription Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)Alternative name(s): Polymerase basic protein 1UniRule annotation Short name: PB1UniRule annotation RNA-directed RNA polymerase subunit P1UniRule annotation |
Gene namesi | Name:PB1UniRule annotation |
Organismi | Influenza A virus (strain A/USA:Iowa/1943 H1N1) |
Taxonomic identifieri | 425563 [NCBI] |
Taxonomic lineagei | Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Alphainfluenzavirus › |
Virus hosti | Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation
- Host cytoplasm UniRule annotation
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000373049 | 1 – 757 | RNA-directed RNA polymerase catalytic subunitAdd BLAST | 757 |
Post-translational modificationi
Phosphorylated by host PRKCA.UniRule annotation
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 286 – 483 | RdRp catalyticUniRule annotationAdd BLAST | 198 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 249 – 256 | Promoter-binding siteUniRule annotation | 8 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 187 – 195 | Nuclear localization signalUniRule annotation | 9 | |
Motifi | 203 – 216 | Nuclear localization signalUniRule annotationAdd BLAST | 14 |
Sequence similaritiesi
Belongs to the influenza viruses polymerase PB1 family.UniRule annotation
Phylogenomic databases
OrthoDBi | VOG09000028 |
Family and domain databases
HAMAPi | MF_04065 INFV_RDRP, 1 hit |
InterProi | View protein in InterPro IPR007099 RNA-dir_pol_NSvirus IPR001407 RNA_pol_PB1_influenza |
Pfami | View protein in Pfam PF00602 Flu_PB1, 1 hit |
PIRSFi | PIRSF000827 RdRPol_OMV, 1 hit |
PROSITEi | View protein in PROSITE PS50525 RDRP_SSRNA_NEG_SEG, 1 hit |
i Sequence
Sequence statusi: Complete.
A4GCL6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMDKEEME ITTHFQRKRR VRDNVTKKMV
210 220 230 240 250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLFN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG
660 670 680 690 700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI
EELRRQK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CY020467 Viral cRNA Translation: ABO38381.1 |
Similar proteinsi
Entry informationi
Entry namei | RDRP_I43A0 | |
Accessioni | A4GCL6Primary (citable) accession number: A4GCL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | April 17, 2007 | |
Last modified: | April 25, 2018 | |
This is version 39 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |