PB1

Functionⁱ

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityⁱ

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functionⁱ

nucleotide binding Source: UniProtKB-KW
RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
transcription, DNA-templated Source: InterPro
viral RNA genome replication Source: InterPro
viral transcription Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Molecular function	Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological process	Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
Ligand	Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation) Alternative name(s): Polymerase basic protein 1UniRule annotation Short name: PB1UniRule annotation RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesⁱ	Name:PB1UniRule annotation
Organismⁱ	Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Taxonomic identifierⁱ	425563 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype
Virus hostⁱ	Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823]
Proteomesⁱ	UP000008432 Componentⁱ: Genome

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
host cell nucleus Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Host cytoplasm, Host nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000373049	1 – 757	RNA-directed RNA polymerase catalytic subunitAdd BLAST		757

Post-translational modificationⁱ

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMⁱ

Phosphoprotein

Interactionⁱ

Subunit structureⁱ

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Structureⁱ

3D structure databases

SMRⁱ	A4GCL6.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	286 – 483	RdRp catalyticUniRule annotationAdd BLAST		198

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	249 – 256	Promoter-binding siteUniRule annotation		8

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	187 – 195	Nuclear localization signalUniRule annotation		9
Motifⁱ	203 – 216	Nuclear localization signalUniRule annotationAdd BLAST		14

Sequence similaritiesⁱ

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG09000028.

OrthoDBⁱ

VOG09000028.

Family and domain databases

HAMAPⁱ	MF_04065. INFV_RDRP. 1 hit.
InterProⁱ	View protein in InterPro IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00602. Flu_PB1. 1 hit.
PIRSFⁱ	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A4GCL6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS 
        60         70         80         90        100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 
       110        120        130        140        150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 
       160        170        180        190        200
RSNGLTANES GRLIDFLKDV MESMDKEEME ITTHFQRKRR VRDNVTKKMV 
       210        220        230        240        250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 
       260        270        280        290        300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 
       310        320        330        340        350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 
       360        370        380        390        400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT 
       410        420        430        440        450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 
       460        470        480        490        500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 
       510        520        530        540        550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 
       560        570        580        590        600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLFN 
       610        620        630        640        650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG 
       660        670        680        690        700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF 
       710        720        730        740        750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 

EELRRQK

Length:757

Mass (Da):86,624

Last modified:April 17, 2007 - v1

Checksum:ⁱ4761F24C01C42638

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA. Translation: ABO38381.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA. Translation: ABO38381.1.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA. Translation: ABO38381.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA. Translation: ABO38381.1.

3D structure databases

SMRⁱ	A4GCL6.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG09000028.

OrthoDBⁱ

VOG09000028.

Family and domain databases

HAMAPⁱ	MF_04065. INFV_RDRP. 1 hit.
InterProⁱ	View protein in InterPro IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00602. Flu_PB1. 1 hit.
PIRSFⁱ	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RDRP_I43A0
Accessionⁱ	A4GCL6Primary (citable) accession number: A4GCL6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
	Last sequence update:	April 17, 2007
	Last modified:	June 7, 2017
	This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A4GCL6 (RDRP_I43A0)

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RNA-directed RNA polymerase catalytic subunit

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ