PB1

Functionⁱ

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityⁱ

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functionⁱ

nucleotide binding Source: UniProtKB-KW
RNA binding Source: InterPro
RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
viral RNA genome replication Source: InterPro
viral transcription Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological process	Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
Ligand	Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation) Alternative name(s): Polymerase basic protein 1UniRule annotation Short name: PB1UniRule annotation RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesⁱ	Name:PB1UniRule annotation
Organismⁱ	Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Taxonomic identifierⁱ	425563 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Alphainfluenzavirus › Influenza A virus › H1N1 subtype
Virus hostⁱ	Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823]
Proteomesⁱ	UP000008432 Componentⁱ: Genome

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
host cell nucleus Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Host nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000373049	1 – 757	RNA-directed RNA polymerase catalytic subunitAdd BLAST		757

Post-translational modificationⁱ

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMⁱ

Phosphoprotein

Interactionⁱ

Subunit structureⁱ

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Structureⁱ

3D structure databases

SMRⁱ	A4GCL6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	286 – 483	RdRp catalyticUniRule annotationAdd BLAST		198

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	249 – 256	Promoter-binding siteUniRule annotation		8

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	187 – 195	Nuclear localization signalUniRule annotation		9
Motifⁱ	203 – 216	Nuclear localization signalUniRule annotationAdd BLAST		14

Sequence similaritiesⁱ

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG09000028

OrthoDBⁱ

VOG09000028

Family and domain databases

HAMAPⁱ	MF_04065 INFV_RDRP, 1 hit
InterProⁱ	View protein in InterPro IPR007099 RNA-dir_pol_NSvirus IPR001407 RNA_pol_PB1_influenza
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00602 Flu_PB1, 1 hit
PIRSFⁱ	PIRSF000827 RdRPol_OMV, 1 hit
PROSITEⁱ	View protein in PROSITE PS50525 RDRP_SSRNA_NEG_SEG, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

A4GCL6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS 
        60         70         80         90        100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 
       110        120        130        140        150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 
       160        170        180        190        200
RSNGLTANES GRLIDFLKDV MESMDKEEME ITTHFQRKRR VRDNVTKKMV 
       210        220        230        240        250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 
       260        270        280        290        300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 
       310        320        330        340        350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 
       360        370        380        390        400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT 
       410        420        430        440        450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 
       460        470        480        490        500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 
       510        520        530        540        550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 
       560        570        580        590        600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLFN 
       610        620        630        640        650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG 
       660        670        680        690        700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF 
       710        720        730        740        750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 

EELRRQK

Length:757

Mass (Da):86,624

Last modified:April 17, 2007 - v1

Checksum:ⁱ4761F24C01C42638

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA Translation: ABO38381.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA Translation: ABO38381.1

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A4GCL6	A4GBX4	Influenza A virus (A/AA/Marton/1943(H1N1))	757	UniRef100_A4GCL6	Cluster: RNA-directed RNA polymerase catalytic subunit	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A4GCL6	Q0A2G8 C6L800 L8B4Y7 I4DXH5 I2FJY3 I2FK02 I4DXG5 I2FK58 I4DST0 E9RJW5 +47569	Influenza A virus (strain A/Chicken/Scotland/1959 H5N1) Influenza A virus (A/duck/Taiwan/TP/0208/2000(H1N1)) Influenza A virus (A/duck/Vietnam/OIE-3022/2011(H3N2)) Influenza A virus (A/duck/Vietnam/OIE-0178/2012(H7N1)) Influenza A virus (A/duck/Vietnam/OIE-3060/2011(H6N2)) Influenza A virus (A/duck/Vietnam/OIE-3349/2011(H6N2)) Influenza A virus (A/duck/Vietnam/OIE-0155/2012(H4N6)) Influenza A virus (A/duck/Vietnam/OIE-3225/2011(H6N2)) Influenza A virus (A/duck/Vietnam/OIE-3344/2011(H6N2)) Influenza A virus (A/duck/Vietnam/OIE-4035/2010(H6N2)) And more	758	UniRef90_Q0A2G8	Cluster: RNA-directed RNA polymerase catalytic subunit	47,580

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A4GCL6	Q9WLS3 D9J326 A0A023ZYS4 E3WEF0 E3WED8 E3WEL6 E3WEE4 E3WEK4 E3WEM8 E3WEJ8 +47695	Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd) Influenza A virus (A/chicken/Uchal/8293/2006(H9N2)) Influenza A virus (A/Uganda/MUWRP-111/2009(H1N1)) Influenza A virus (A/Kobe/9336/2009(H1N1)) Influenza A virus (A/Kobe/9154/2009(H1N1)) Influenza A virus (A/Kobe/91586/2009(H1N1)) Influenza A virus (A/Kobe/9185/2009(H1N1)) Influenza A virus (A/Kobe/91571/2009(H1N1)) Influenza A virus (A/Kobe/91660/2009(H1N1)) Influenza A virus (A/Kobe/91569/2009(H1N1)) And more	758	UniRef50_Q9WLS3	Cluster: RNA-directed RNA polymerase catalytic subunit	47,706

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA Translation: ABO38381.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA Translation: ABO38381.1

3D structure databases

SMRⁱ	A4GCL6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG09000028

OrthoDBⁱ

VOG09000028

Family and domain databases

HAMAPⁱ	MF_04065 INFV_RDRP, 1 hit
InterProⁱ	View protein in InterPro IPR007099 RNA-dir_pol_NSvirus IPR001407 RNA_pol_PB1_influenza
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00602 Flu_PB1, 1 hit
PIRSFⁱ	PIRSF000827 RdRPol_OMV, 1 hit
PROSITEⁱ	View protein in PROSITE PS50525 RDRP_SSRNA_NEG_SEG, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RDRP_I43A0
Accessionⁱ	A4GCL6Primary (citable) accession number: A4GCL6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
	Last sequence update:	April 17, 2007
	Last modified:	April 25, 2018
	This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A4GCL6 (RDRP_I43A0)

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RNA-directed RNA polymerase catalytic subunit

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

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<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

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Sequence databases

3D structure databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ