PB1

Functionⁱ

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.By similarity

Catalytic activityⁱ

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48) Alternative name(s): Polymerase basic protein 1 Short name: PB1 RNA-directed RNA polymerase subunit P1
Gene namesⁱ	Name:PB1
Organismⁱ	Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Taxonomic identifierⁱ	425563 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype
Virus hostⁱ	Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823]
Proteomesⁱ	UP000008432: Genome

Subcellular locationⁱ

Host nucleus By similarity. Host cytoplasm By similarity

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
host cell nucleus Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Host cytoplasm, Host nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Chainⁱ	1 – 757	757	RNA-directed RNA polymerase catalytic subunit		PRO_0000373049	Add BLAST

Post-translational modificationⁱ

Phosphorylated by host PRKCA.By similarity

Keywords - PTMⁱ

Phosphoprotein

Interactionⁱ

Subunit structureⁱ

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.By similarity

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Domainⁱ	286 – 483	198	RdRp catalyticPROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU00539			Add BLAST

Region

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Regionⁱ	249 – 256	8	Promoter-binding siteBy similarity

Motif

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Motifⁱ	187 – 195	9	Nuclear localization signalBy similarity
Motifⁱ	203 – 216	14	Nuclear localization signalBy similarity			Add BLAST

Sequence similaritiesⁱ

Belongs to the influenza viruses polymerase PB1 family.Curated

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProⁱ	IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza. [Graphical view]
Pfamⁱ	PF00602. Flu_PB1. 1 hit. [Graphical view]
PIRSFⁱ	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEⁱ	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]

Sequenceⁱ

Sequence statusⁱ: Complete.

A4GCL6-1 [UniParc]FASTA Add to Basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS 
        60         70         80         90        100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 
       110        120        130        140        150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 
       160        170        180        190        200
RSNGLTANES GRLIDFLKDV MESMDKEEME ITTHFQRKRR VRDNVTKKMV 
       210        220        230        240        250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 
       260        270        280        290        300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 
       310        320        330        340        350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 
       360        370        380        390        400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT 
       410        420        430        440        450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 
       460        470        480        490        500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 
       510        520        530        540        550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 
       560        570        580        590        600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLFN 
       610        620        630        640        650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG 
       660        670        680        690        700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF 
       710        720        730        740        750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 

EELRRQK

Length:757

Mass (Da):86,624

Last modified:April 17, 2007 - v1

Checksum:ⁱ4761F24C01C42638

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA. Translation: ABO38381.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA. Translation: ABO38381.1.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CY020467 Viral cRNA. Translation: ABO38381.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

CY020467 Viral cRNA. Translation: ABO38381.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

InterProⁱ	IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza. [Graphical view]
Pfamⁱ	PF00602. Flu_PB1. 1 hit. [Graphical view]
PIRSFⁱ	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEⁱ	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNetⁱ	Search...

Publicationsⁱ

"The NIAID influenza genome sequencing project."
Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y.
Tatusova T.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
The NIAID Influenza Genome Sequencing Consortium
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationⁱ

Entry nameⁱ

RDRP_I43A0

Accessionⁱ

Primary (citable) accession number: A4GCL6

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 17, 2007
Last modified:	January 7, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

Documents

SIMILARITY comments
Index of protein domains and families

External Data

Dasty 3

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

Proteomes

UniRef

Supporting data

UniParc

Help

A4GCL6 - RDRP_I43A0

UniProt

A4GCL6 - RDRP_I43A0

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RNA-directed RNA polymerase catalytic subunit

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<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>Contains the literature citations that are the sources of data used to annotate the entry. Each reference is numbered and contains several subsections allowing a precise description of a given citation.</p><p><a href='../manual/publications_section' target='_top'>More...</a></p>Publicationsi

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Documents

External Data

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Publicationsⁱ

Entry informationⁱ

Miscellaneousⁱ