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UniProtKB - A4GCL6 (RDRP_I43A0)

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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)
Alternative name(s):
Polymerase basic protein 1UniRule annotation
Short name:
PB1UniRule annotation
RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesi
Name:PB1UniRule annotation
OrganismiInfluenza A virus (strain A/USA:Iowa/1943 H1N1)
Taxonomic identifieri425563 [NCBI]
Taxonomic lineageiViruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000008432 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003730491 – 757RNA-directed RNA polymerase catalytic subunitAdd BLAST757

Post-translational modificationi

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Structurei

3D structure databases

SMRiA4GCL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 483RdRp catalyticUniRule annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 256Promoter-binding siteUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 195Nuclear localization signalUniRule annotation9
Motifi203 – 216Nuclear localization signalUniRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG09000028.

Family and domain databases

HAMAPiMF_04065. INFV_RDRP. 1 hit.
InterProiView protein in InterPro
IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
PfamiView protein in Pfam
PF00602. Flu_PB1. 1 hit.
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiView protein in PROSITE
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.

Sequencei

Sequence statusi: Complete.

A4GCL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS 
        60         70         80         90        100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 
       110        120        130        140        150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 
       160        170        180        190        200
RSNGLTANES GRLIDFLKDV MESMDKEEME ITTHFQRKRR VRDNVTKKMV 
       210        220        230        240        250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 
       260        270        280        290        300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 
       310        320        330        340        350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 
       360        370        380        390        400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT 
       410        420        430        440        450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 
       460        470        480        490        500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 
       510        520        530        540        550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 
       560        570        580        590        600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLFN 
       610        620        630        640        650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG 
       660        670        680        690        700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF 
       710        720        730        740        750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 

EELRRQK
Length:757
Mass (Da):86,624
Last modified:April 17, 2007 - v1
Checksum:i4761F24C01C42638
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020467 Viral cRNA. Translation: ABO38381.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020467 Viral cRNA. Translation: ABO38381.1.

3D structure databases

SMRiA4GCL6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG09000028.

Family and domain databases

HAMAPiMF_04065. INFV_RDRP. 1 hit.
InterProiView protein in InterPro
IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
PfamiView protein in Pfam
PF00602. Flu_PB1. 1 hit.
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiView protein in PROSITE
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_I43A0
AccessioniPrimary (citable) accession number: A4GCL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 17, 2007
Last modified: June 7, 2017
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.