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A4GCL2

- NCAP_I43A0

UniProt

A4GCL2 - NCAP_I43A0

Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/USA:Iowa/1943 H1N1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    Protein N
    Gene namesi
    Name:NP
    OrganismiInfluenza A virus (strain A/USA:Iowa/1943 H1N1)
    Taxonomic identifieri425563 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008432: Genome

    Subcellular locationi

    Virion Curated. Host nucleus By similarity

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell nucleus Source: UniProtKB-SubCell
    3. intracellular Source: GOC
    4. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498NucleoproteinPRO_0000372936Add
    BLAST

    Interactioni

    Subunit structurei

    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA4GCL2.
    SMRiA4GCL2. Positions 22-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
    BLAST
    Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002141. Flu_NP.
    [Graphical view]
    PfamiPF00506. Flu_NP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4GCL2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQGTKRSY EQMETDGERQ NATEIRASVG KMIGGIGRFY IQMCTELKLS    50
    DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV 100
    DGKWMRELIL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDATYQR 150
    TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
    INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
    AEFEDLTFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG 300
    IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT 350
    RVVPRGKLST RGVQIASNEN METMGSSTLE LRSRYWAIRT RSGGNTNQQR 400
    ASAGQISIQP TFSVQRNLPF DRTTIMAAFT GNTEGRTSDM RTEIIRMMES 450
    ARPEDVSFQG RGVFELSDEK AANPIVPSFD MSNEGSYFFG DNAEEYDN 498
    Length:498
    Mass (Da):56,155
    Last modified:April 17, 2007 - v1
    Checksum:iBDBB49CDD5D5B47E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CY020464 Viral cRNA. Translation: ABO38377.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CY020464 Viral cRNA. Translation: ABO38377.1 .

    3D structure databases

    ProteinModelPortali A4GCL2.
    SMRi A4GCL2. Positions 22-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002141. Flu_NP.
    [Graphical view ]
    Pfami PF00506. Flu_NP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. The NIAID Influenza Genome Sequencing Consortium
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNCAP_I43A0
    AccessioniPrimary (citable) accession number: A4GCL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3