A4GCK6 (PB1F2_I80AA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein PB1-F2

Gene names

Name:

PB1-F2

Organism

Influenza A virus (strain A/India/6263/1980 H1N1) [Complete proteome]

Taxonomic identifier

393562 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	57 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis By similarity.
Subunit structure	Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1 By similarity.
Subcellular location	Host mitochondrion inner membrane. Host nucleus. Host cytoplasm › host cytosol. Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol By similarity.
Miscellaneous	Is not encoded in all strains, and seems to be dispensable for replication.
Sequence similarities	Belongs to the influenza viruses PB1-F2 family.

Ontologies

Keywords
Biological process	Apoptosis Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host MAVS by virus Inhibition of host innate immune response by virus Modulation of host cell apoptosis by virus Viral immunoevasion
Cellular component	Host cytoplasm Host membrane Host mitochondrion Host mitochondrion inner membrane Host nucleus Membrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW modulation by virus of host apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host MAVS activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell host cell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 57	57	Protein PB1-F2		PRO_0000373023

Sequences

	Sequence		Length	Mass (Da)
	A4GCK6 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: F640088800E69BA2	FASTA	57	6,540
	10 20 30 40 50 MGQEQGTPWI QSTGHISTQK GEDGQKTPKL EHRNSTRLMG HYQKTMNQVV MPKQIVY

References

[1]

"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. , Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

[2]

The NIAID Influenza Genome Sequencing Consortium Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CY020459 Viral cRNA. Translation: ABO38371.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR021045. Flu_proapoptotic_PB1-F2. [Graphical view]
Pfam	PF11986. PB1-F2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PB1F2_I80AA

Accession

Primary (citable) accession number: A4GCK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 17, 2007
Last modified:	January 9, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program