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A4GCI3

- RDRP_I83A1

UniProt

A4GCI3 - RDRP_I83A1

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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Chile/1/1983 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
Alternative name(s):
Polymerase basic protein 1
Short name:
PB1
RNA-directed RNA polymerase subunit P1
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Chile/1/1983 H1N1)
Taxonomic identifieri380985 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008582: Genome

Subcellular locationi

Host nucleus By similarity. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000373057Add
BLAST

Post-translational modificationi

Phosphorylated by host PRKCA.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA4GCI3.

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni249 – 2568Promoter-binding siteBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1959Nuclear localization signalBy similarity
Motifi203 – 21614Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4GCI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
ERGRWTKNTE TGAPQLNPID GPLPKDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLMANES GRLIDFLKDV MDSMDREEME ITTHFQRKRR VRDNVTKKMV
210 220 230 240 250
TQRTIGKKKH RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNILSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKLRTQIPA EMLANIDLRY FNDSTRKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNYAGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW DQTRSKTGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWDLMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG
660 670 680 690 700
PAKIMEYDAV ATTHSWVPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMIS RARIDARIDF ESGRIKKEEF TEIMKTCSTI

EELRRQK
Length:757
Mass (Da):86,672
Last modified:April 17, 2007 - v1
Checksum:i66DBF070BCE4440A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020443 Viral cRNA. Translation: ABO38348.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020443 Viral cRNA. Translation: ABO38348.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi A4GCI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. The NIAID Influenza Genome Sequencing Consortium
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_I83A1
AccessioniPrimary (citable) accession number: A4GCI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 17, 2007
Last modified: October 29, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3