A4GCH9 (NCAP_I83A1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein

Alternative name(s):
Nucleocapsid protein
Short name=Protein N

Gene names

Name:

NP

Organism

Influenza A virus (strain A/Chile/1/1983 H1N1) [Complete proteome]

Taxonomic identifier

380985 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.
Subunit structure	Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.
Subcellular location	Virion Potential. Host nucleus By similarity.
Sequence similarities	Belongs to the influenza viruses nucleoprotein family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral penetration into host nucleus Virus entry into host cell
Cellular component	Host nucleus Virion
Ligand	RNA-binding Viral nucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral penetration into host nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Nucleoprotein		PRO_0000372942
Regions
	Motif	1 – 18	18	Unconventional nuclear localization signal By similarity
	Motif	198 – 216	19	Bipartite nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4GCH9 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 922F62A0B1873ED9	FASTA	498	56,020
	10 20 30 40 50 60 MASQGTKRSY EQMETDGERQ NATEIRASVG KMIGGIGRFY IQMCTELKLN DYEGRLIQNS 70 80 90 100 110 120 LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV DGKWMRXLVL YDKEEIRRIW 130 140 150 160 170 180 RQANNGDDAT AGLTHIMIWH SNLNDTTYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG 190 200 210 220 230 240 AAVKGVGTMV LELIRMIKRG INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD 250 260 270 280 290 300 QVRESRNPGN AEIEDLTFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEKEGYSLVG 310 320 330 340 350 360 IDPFKLLQTS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVSSFIRGT KVIPRGKLST 370 380 390 400 410 420 RGVQIASNEN MDTMVSSTLE LRSRYWAIRT RSGGNTNQQR ASAGQISIQP TFSVQRNLPF 430 440 450 460 470 480 DKTTIMAAFT GNAEGRTSDM RAEIIKMMES ARPEEVSFQG RGVFEFSDER AANPIVPSFD 490 MSNEGSYFFG DNAEEYDN

References

[1]

"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. , Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

[2]

The NIAID Influenza Genome Sequencing Consortium Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CY020440 Viral cRNA. Translation: ABO38344.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002141. Flu_NP. [Graphical view]
Pfam	PF00506. Flu_NP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCAP_I83A1

Accession

Primary (citable) accession number: A4GCH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 17, 2007
Last modified:	January 9, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program