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Protein

Matrix protein 1

Gene

M

Organism
Influenza A virus (strain A/Chile/1/1983 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place (By similarity).By similarity
Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host complement activation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host complement factors by virus, Viral immunoevasion

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein 1
Short name:
M1
Gene namesi
Name:M
OrganismiInfluenza A virus (strain A/Chile/1/1983 H1N1)
Taxonomic identifieri380985 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008582 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Matrix protein 1PRO_0000372912Add
BLAST

Interactioni

Subunit structurei

Homodimer and homomultimer. Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA. Cannot bind NP without genomic RNA (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA4GCH7.
SMRiA4GCH7. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 164164Membrane-bindingBy similarityAdd
BLAST
Regioni165 – 25288RNP-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi101 – 1055Nuclear localization signalBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.180. 1 hit.
1.20.91.10. 1 hit.
InterProiIPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015423. Flu_matrix_M1_N_sub1.
IPR015799. Flu_matrix_M1_N_sub2.
[Graphical view]
PfamiPF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
[Graphical view]
ProDomiPD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00759. Flu_M1_C. 1 hit.
[Graphical view]
SUPFAMiSSF48145. SSF48145. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Only the first 9 residues are shared by the 2 isoforms.

Isoform M1 (identifier: A4GCH7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLTEVETY VLSIVPSGPL KAEIAQRLED VFAGKNTDLE ALMEWLKTRP
60 70 80 90 100
ILSPLTKGIL GFVFTLTVPS ERGLQRRRFV QNALNGNGDP NNMDRAVKLY
110 120 130 140 150
RKLKREITFH GAKEIALSYS AGALASCMGL IYNRMGAVTT ESAFGLICAT
160 170 180 190 200
CEQIADSQHK SHRQMVITTN PLIRHENRMV LASTTAKAME QMAGSSEQAA
210 220 230 240 250
EAMEVASQAR QMVQAMRAIG THPSSSTGLK NDLLENLQAY QKRMGVQMQR

FK
Length:252
Mass (Da):27,849
Last modified:April 17, 2007 - v1
Checksum:i3C4CA792DC915D4D
GO
Isoform M2 (identifier: A4GCH6-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry A4GCH6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:97
Mass (Da):11,149
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020438 Viral cRNA. Translation: ABO38341.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY020438 Viral cRNA. Translation: ABO38341.1.

3D structure databases

ProteinModelPortaliA4GCH7.
SMRiA4GCH7. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.10.180. 1 hit.
1.20.91.10. 1 hit.
InterProiIPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015423. Flu_matrix_M1_N_sub1.
IPR015799. Flu_matrix_M1_N_sub2.
[Graphical view]
PfamiPF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
[Graphical view]
ProDomiPD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00759. Flu_M1_C. 1 hit.
[Graphical view]
SUPFAMiSSF48145. SSF48145. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. The NIAID Influenza Genome Sequencing Consortium
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiM1_I83A1
AccessioniPrimary (citable) accession number: A4GCH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 17, 2007
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.