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A4G916 (FPG_HERAR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase

Short name=Fapy-DNA glycosylase
EC=3.2.2.23
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM
Short name=AP lyase MutM
EC=4.2.99.18
Gene names
Name:mutM
Synonyms:fpg
Ordered Locus Names:HEAR2889
OrganismHerminiimonas arsenicoxydans [Complete proteome] [HAMAP]
Taxonomic identifier204773 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 273272Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
PRO_1000008704

Regions

Zinc finger239 – 27335FPG-type HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site581Proton donor; for beta-elimination activity By similarity
Active site2631Proton donor; for delta-elimination activity By similarity
Binding site911DNA By similarity
Binding site1091DNA By similarity
Binding site1541DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
A4G916 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 665F2BB4CC6DAE8D

FASTA27330,353
        10         20         30         40         50         60 
MPELPEVEVT RRGVAPYLEG QVVSGVVLRH TGLRWPFPAA LSQTLAGRTI RSTGRRGKYL 

        70         80         90        100        110        120 
LIHFDHGTLI VHLGMSGHIR ILPLGVPPQK HDHFDMTVGN QVLRLTDPRR FGAVLWHAAE 

       130        140        150        160        170        180 
EGAVDQHLLL RTLGVEPLEG LFTAQWLYRQ TRSRRSAIKQ VLLAGDIVVG VGNIYASESL 

       190        200        210        220        230        240 
FQAGINPKTQ AHRIGLQRYE RLAQAIREIL AAAIAQGGST LKDFIGVNGQ SGYFQQNYFV 

       250        260        270 
YARTGEPCRI CNAPVRQIVQ GQRSTFYCPN CQK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU207211 Genomic DNA. Translation: CAL63003.1.
RefSeqYP_001101124.1. NC_009138.1.

3D structure databases

ProteinModelPortalA4G916.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204773.HEAR2889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL63003; CAL63003; HEAR2889.
GeneID4930617.
KEGGhar:HEAR2889.
PATRIC22115738. VBIHerArs17568_2727.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000020881.
KOK10563.
OMAIYCSESL.
OrthoDBEOG6QP131.

Enzyme and pathway databases

BioCycHARS204773:GJCA-2783-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFPG_HERAR
AccessionPrimary (citable) accession number: A4G916
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families