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Protein

Glutamate 5-kinase

Gene

proB

Organism
Herminiimonas arsenicoxydans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATPUniRule annotation
Binding sitei54 – 541SubstrateUniRule annotation
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei153 – 1531Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi173 – 1742ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHARS204773:GJCA-2680-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:HEAR2784
OrganismiHerminiimonas arsenicoxydans
Taxonomic identifieri204773 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas
ProteomesiUP000006697 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Glutamate 5-kinasePRO_1000081066Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi204773.HEAR2784.

Structurei

3D structure databases

ProteinModelPortaliA4G8R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 35879PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4G8R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSAIQSAKR IIIKVGSSLV TNDGKGLDTA AIQKWAEQIV QLRALGKEVV
60 70 80 90 100
LVSSGAIAEG MQRLGFDKRP TGVHELQACA AVGQMGLAQI YETSFRQHQV
110 120 130 140 150
QTAQILLTHA DLADRERYLN ARSTLFTLLQ LGVVPIINEN DTVVTDEIKF
160 170 180 190 200
GDNDTLGALV ANLIEADALI ILTDQRGLFS ADPRKDPHAT FIHEAQAGDV
210 220 230 240 250
ELEAMAGGAG SSLGRGGMLT KILAAKRAAL SGAHTVIAWG REEQVLTRLA
260 270 280 290 300
AGEAIGTQLT AQTAQLTARK QWMADHLHTA GRIVLDQGAV LKLTAEGKSL
310 320 330 340 350
LPIGVIEVSG SFGRGDVITC VDQSGRAIAR GITNYTSSEA RRIMRKPSTD
360 370
ILSILGFVEE PELIHRDNLV LL
Length:372
Mass (Da):39,792
Last modified:April 17, 2007 - v1
Checksum:i602113EF757D1AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207211 Genomic DNA. Translation: CAL62900.1.
RefSeqiWP_011872157.1. NC_009138.1.
YP_001101021.1. NC_009138.1.

Genome annotation databases

EnsemblBacteriaiCAL62900; CAL62900; HEAR2784.
KEGGihar:HEAR2784.
PATRICi22115524. VBIHerArs17568_2620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207211 Genomic DNA. Translation: CAL62900.1.
RefSeqiWP_011872157.1. NC_009138.1.
YP_001101021.1. NC_009138.1.

3D structure databases

ProteinModelPortaliA4G8R3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204773.HEAR2784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL62900; CAL62900; HEAR2784.
KEGGihar:HEAR2784.
PATRICi22115524. VBIHerArs17568_2620.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciHARS204773:GJCA-2680-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ULPAs1.

Entry informationi

Entry nameiPROB_HERAR
AccessioniPrimary (citable) accession number: A4G8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.