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A4G778 (SYK_HERAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:HEAR2230
OrganismHerminiimonas arsenicoxydans [Complete proteome] [HAMAP]
Taxonomic identifier204773 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00252

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511Lysine--tRNA ligase HAMAP-Rule MF_00252
PRO_1000012878

Sites

Metal binding4211Magnesium 1 By similarity
Metal binding4281Magnesium 1 By similarity
Metal binding4281Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4G778 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 03AA2433E962064F

FASTA51157,531
        10         20         30         40         50         60 
MTTHNDDAAA SLPIDENKII AERRAKLSAL REQGIAFPND FRPQHKAADL QEKYGSKTRE 

        70         80         90        100        110        120 
ELEAEPVTVV LAGRMMLKRE AGKKAAFATL QDASGSKADG RIQIYVTLNL TGEAAMAALH 

       130        140        150        160        170        180 
HYDLGDILGV SGTLFKTKTD ELTIKVSELR LITKSLRPLP DKFHGLADQE TKYRQRYVDL 

       190        200        210        220        230        240 
IMNEDTRRTF KARTAAISSI RRFMERNDFM EVETPMLHTI PGGAAAKPFT THHNALDMEM 

       250        260        270        280        290        300 
FLRIAPELYL KRLVVGGFDR VFEINRNFRN EGVSIRHNPE FTMMEFYAAY TDYKWLMDFT 

       310        320        330        340        350        360 
EAVIRQAAID AHGTATLTYG GRELDLAKPF HRLTIVGAIN KFAPQYTHEQ LHDAEYIKAE 

       370        380        390        400        410        420 
LKKFGVKPHA HSGLGALQLA LFEETAEAQL WEPTYIIDYP VEVSPLARAS DTVAGITERF 

       430        440        450        460        470        480 
ELFMVGREIA NGFSELNDAE DQAARFQAQV AAKDAGDEEA MYYDADYIRA LEYGMPPTGG 

       490        500        510 
CGIGIDRLMM IITDSPNIRD VLLFPHLRRE D 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU207211 Genomic DNA. Translation: CAL62365.1.
RefSeqYP_001100488.1. NC_009138.1.

3D structure databases

ProteinModelPortalA4G778.
SMRA4G778. Positions 14-508.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204773.HEAR2230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL62365; CAL62365; HEAR2230.
GeneID4932131.
KEGGhar:HEAR2230.
PATRIC22114474. VBIHerArs17568_2101.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1190.
HOGENOMHOG000236578.
KOK04567.
OMADLMDFTE.
OrthoDBEOG69PQ2M.
ProtClustDBPRK00484.

Enzyme and pathway databases

BioCycHARS204773:GJCA-2147-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF4. PTHR22594:SF4. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_HERAR
AccessionPrimary (citable) accession number: A4G778
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries