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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Herminiimonas arsenicoxydans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHARS204773:GJCA-937-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:HEAR0981
OrganismiHerminiimonas arsenicoxydans
Taxonomic identifieri204773 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas
ProteomesiUP000006697: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Glutamate--tRNA ligasePRO_0000330974Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi204773.HEAR0981.

Structurei

3D structure databases

ProteinModelPortaliA4G3S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 2311"HIGH" regionAdd
BLAST
Motifi245 – 2495"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiIEAFKWV.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4G3S7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTARTPIRTR FAPSPTGYLH VGGARTALFS WAYARHFGGT FVLRIEDTDL
60 70 80 90 100
ERSTPEAVQA IIEGMEWLGL HHDEGPFYQM QRMDRYREVI AQMLAAGTAY
110 120 130 140 150
YCYSSSEEVE AMRERQRAAG EKPRYDGTWR PEAGKTLPAI PADRKPVVRF
160 170 180 190 200
RNPMEGDVTW LDVVKGQITI SNRELDDLVI ARQDGTPTYN FCVAVDDSDM
210 220 230 240 250
KITHVIRGDD HVNNTPRQIN ILKALGAELP HYGHLPMILG ADGAKLSKRH
260 270 280 290 300
GAVSVMDYPA QGYLPEAMLN YLARLGWSHG DDEVFSMEQF TEWFDLDHLT
310 320 330 340 350
KSPAQFDPEK LDWINNHYIK QADNTRLAGL IKPMMENLGA QFDNAPDLPA
360 370 380 390 400
VIGLMKERVK TLNELAVTAM LFYRQPAPDA ALLTQHLTDA VKPALAQYVE
410 420 430 440 450
QLKTVAWGKD ALSAALKEVL ATHKLKMPQL AMPLRLIITG QLQTPSIDAV
460
VELFGREVVL ARIANYL
Length:467
Mass (Da):52,411
Last modified:April 17, 2007 - v1
Checksum:iC95B060330F61B14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207211 Genomic DNA. Translation: CAL61164.1.
RefSeqiWP_011870501.1. NC_009138.1.
YP_001099291.1. NC_009138.1.

Genome annotation databases

EnsemblBacteriaiCAL61164; CAL61164; HEAR0981.
GeneIDi4931394.
KEGGihar:HEAR0981.
PATRICi22112052. VBIHerArs17568_0908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207211 Genomic DNA. Translation: CAL61164.1.
RefSeqiWP_011870501.1. NC_009138.1.
YP_001099291.1. NC_009138.1.

3D structure databases

ProteinModelPortaliA4G3S7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204773.HEAR0981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL61164; CAL61164; HEAR0981.
GeneIDi4931394.
KEGGihar:HEAR0981.
PATRICi22112052. VBIHerArs17568_0908.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiIEAFKWV.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciHARS204773:GJCA-937-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ULPAs1.

Entry informationi

Entry nameiSYE_HERAR
AccessioniPrimary (citable) accession number: A4G3S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 17, 2007
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.