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A4G3S7 (SYE_HERAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:HEAR0981
OrganismHerminiimonas arsenicoxydans [Complete proteome] [HAMAP]
Taxonomic identifier204773 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000330974

Regions

Motif13 – 2311"HIGH" region HAMAP-Rule MF_00022
Motif245 – 2495"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4G3S7 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: C95B060330F61B14

FASTA46752,411
        10         20         30         40         50         60 
MTARTPIRTR FAPSPTGYLH VGGARTALFS WAYARHFGGT FVLRIEDTDL ERSTPEAVQA 

        70         80         90        100        110        120 
IIEGMEWLGL HHDEGPFYQM QRMDRYREVI AQMLAAGTAY YCYSSSEEVE AMRERQRAAG 

       130        140        150        160        170        180 
EKPRYDGTWR PEAGKTLPAI PADRKPVVRF RNPMEGDVTW LDVVKGQITI SNRELDDLVI 

       190        200        210        220        230        240 
ARQDGTPTYN FCVAVDDSDM KITHVIRGDD HVNNTPRQIN ILKALGAELP HYGHLPMILG 

       250        260        270        280        290        300 
ADGAKLSKRH GAVSVMDYPA QGYLPEAMLN YLARLGWSHG DDEVFSMEQF TEWFDLDHLT 

       310        320        330        340        350        360 
KSPAQFDPEK LDWINNHYIK QADNTRLAGL IKPMMENLGA QFDNAPDLPA VIGLMKERVK 

       370        380        390        400        410        420 
TLNELAVTAM LFYRQPAPDA ALLTQHLTDA VKPALAQYVE QLKTVAWGKD ALSAALKEVL 

       430        440        450        460 
ATHKLKMPQL AMPLRLIITG QLQTPSIDAV VELFGREVVL ARIANYL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU207211 Genomic DNA. Translation: CAL61164.1.
RefSeqYP_001099291.1. NC_009138.1.

3D structure databases

ProteinModelPortalA4G3S7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204773.HEAR0981.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL61164; CAL61164; HEAR0981.
GeneID4931394.
KEGGhar:HEAR0981.
PATRIC22112052. VBIHerArs17568_0908.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMARANQGKF.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycHARS204773:GJCA-937-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_HERAR
AccessionPrimary (citable) accession number: A4G3S7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 17, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries