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A4G3E6

- SYI_HERAR

UniProt

A4G3E6 - SYI_HERAR

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Herminiimonas arsenicoxydans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931Aminoacyl-adenylateUniRule annotation
Binding sitei637 – 6371ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHARS204773:GJCA-806-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HEAR0845
OrganismiHerminiimonas arsenicoxydans
Taxonomic identifieri204773 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas
ProteomesiUP000006697: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000022079Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi204773.HEAR0845.

Structurei

3D structure databases

ProteinModelPortaliA4G3E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 8411"HIGH" regionAdd
BLAST
Motifi634 – 6385"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4G3E6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDNQNKPGK PNKPEAKQAS KYPVNMTDTS FPMRGDLAKR EPQWVKQWQD
60 70 80 90 100
KKIYEKIREA SKGRPKFILH DGPPYANGDI HIGHAVNKIL KDMIVKTRQF
110 120 130 140 150
DGYDAPYVPG WDCHGMPIEI QIEKLYGKSL PTAEVLEKSR AYATEQIERQ
160 170 180 190 200
KKDFIRLGVL GEWDNPYKTM DFGNEADEIR ALGTLLEKGY VYRGLKPVNW
210 220 230 240 250
CFDCGSALAE AEVEYQDKRD PAIDVGFPFA EPEKVAKAFG LAKLPTNKGY
260 270 280 290 300
AVIWTTTPWT IPANQALNVH PEFTYALVQT ERKGETVLLI LAADLVEATL
310 320 330 340 350
QRYGLEGKTI ATCVGEALAM IKFKHPFADV DPGYNRLSPI YPGTYVTADS
360 370 380 390 400
GTGIVHSAPA YGIEDFISCK SHGLKDDEIV APVMADGKYA SWLPLFGGLT
410 420 430 440 450
IWEASKPICA KLDEVGSLFK LVMFDHSYMH CWRHKTPIIY RATSQWFAGM
460 470 480 490 500
DVMPKNQGTT LRETALAAIE QTEFFPGWGK ARLHGMIANR PDWTLSRQRQ
510 520 530 540 550
WGVPMAFFVH KESGDLHPRT PELLEQIAQR VEKNGIEAWL KLDPKELLGD
560 570 580 590 600
DADMYLKNKD TLDVWFDSGT THQTVLRGSH KEQLAFPADL YLEGSDQHRG
610 620 630 640 650
WFHSSLLTSS MLNGHAPYKA LLTHGFVVDG EGKKMSKSKG NVVAPQKVSD
660 670 680 690 700
SLGADILRLW VAATDYSGEL SISDEILKRV TESYRRIRNT LRFLLSNTSD
710 720 730 740 750
FDAGKDLVAV ADMLEIDRYA IAQMNAMQNE IVAHYRAYEF HPVISKLQGY
760 770 780 790 800
CTEDLGGFYL DILKDRLYTS GVTSHARRSA QSAIWHLTQS LLRLMAPVLS
810 820 830 840 850
FTAEEAWVIF AGKDAGDTIF TQTYYALPEV ADGDALLGKY TLLREVRNDV
860 870 880 890 900
TKQLEEVRVA GGIGSSLQAE VALQASGDKF AALESLDDDL KFVLITSQAS
910 920 930 940 950
VKKVAGTEEE SVLVTPSTYQ KCERCWHYRA DVGSHAEHEG LCGRCVANLF

GKGEARHFA
Length:959
Mass (Da):107,225
Last modified:April 17, 2007 - v1
Checksum:i12FF0306677286CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU207211 Genomic DNA. Translation: CAL61033.1.
RefSeqiWP_011870373.1. NC_009138.1.
YP_001099160.1. NC_009138.1.

Genome annotation databases

EnsemblBacteriaiCAL61033; CAL61033; HEAR0845.
GeneIDi4931905.
KEGGihar:HEAR0845.
PATRICi22111792. VBIHerArs17568_0780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU207211 Genomic DNA. Translation: CAL61033.1 .
RefSeqi WP_011870373.1. NC_009138.1.
YP_001099160.1. NC_009138.1.

3D structure databases

ProteinModelPortali A4G3E6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 204773.HEAR0845.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL61033 ; CAL61033 ; HEAR0845 .
GeneIDi 4931905.
KEGGi har:HEAR0845.
PATRICi 22111792. VBIHerArs17568_0780.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HARS204773:GJCA-806-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ULPAs1.

Entry informationi

Entry nameiSYI_HERAR
AccessioniPrimary (citable) accession number: A4G3E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3