Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A4G3E6

- SYI_HERAR

UniProt

A4G3E6 - SYI_HERAR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Herminiimonas arsenicoxydans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei593 – 5931Aminoacyl-adenylateUniRule annotation
    Binding sitei637 – 6371ATPUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi942 – 9421ZincUniRule annotation
    Metal bindingi945 – 9451ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHARS204773:GJCA-806-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HEAR0845
    OrganismiHerminiimonas arsenicoxydans
    Taxonomic identifieri204773 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeHerminiimonas
    ProteomesiUP000006697: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000022079Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi204773.HEAR0845.

    Structurei

    3D structure databases

    ProteinModelPortaliA4G3E6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi74 – 8411"HIGH" regionAdd
    BLAST
    Motifi634 – 6385"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4G3E6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNQNKPGK PNKPEAKQAS KYPVNMTDTS FPMRGDLAKR EPQWVKQWQD    50
    KKIYEKIREA SKGRPKFILH DGPPYANGDI HIGHAVNKIL KDMIVKTRQF 100
    DGYDAPYVPG WDCHGMPIEI QIEKLYGKSL PTAEVLEKSR AYATEQIERQ 150
    KKDFIRLGVL GEWDNPYKTM DFGNEADEIR ALGTLLEKGY VYRGLKPVNW 200
    CFDCGSALAE AEVEYQDKRD PAIDVGFPFA EPEKVAKAFG LAKLPTNKGY 250
    AVIWTTTPWT IPANQALNVH PEFTYALVQT ERKGETVLLI LAADLVEATL 300
    QRYGLEGKTI ATCVGEALAM IKFKHPFADV DPGYNRLSPI YPGTYVTADS 350
    GTGIVHSAPA YGIEDFISCK SHGLKDDEIV APVMADGKYA SWLPLFGGLT 400
    IWEASKPICA KLDEVGSLFK LVMFDHSYMH CWRHKTPIIY RATSQWFAGM 450
    DVMPKNQGTT LRETALAAIE QTEFFPGWGK ARLHGMIANR PDWTLSRQRQ 500
    WGVPMAFFVH KESGDLHPRT PELLEQIAQR VEKNGIEAWL KLDPKELLGD 550
    DADMYLKNKD TLDVWFDSGT THQTVLRGSH KEQLAFPADL YLEGSDQHRG 600
    WFHSSLLTSS MLNGHAPYKA LLTHGFVVDG EGKKMSKSKG NVVAPQKVSD 650
    SLGADILRLW VAATDYSGEL SISDEILKRV TESYRRIRNT LRFLLSNTSD 700
    FDAGKDLVAV ADMLEIDRYA IAQMNAMQNE IVAHYRAYEF HPVISKLQGY 750
    CTEDLGGFYL DILKDRLYTS GVTSHARRSA QSAIWHLTQS LLRLMAPVLS 800
    FTAEEAWVIF AGKDAGDTIF TQTYYALPEV ADGDALLGKY TLLREVRNDV 850
    TKQLEEVRVA GGIGSSLQAE VALQASGDKF AALESLDDDL KFVLITSQAS 900
    VKKVAGTEEE SVLVTPSTYQ KCERCWHYRA DVGSHAEHEG LCGRCVANLF 950
    GKGEARHFA 959
    Length:959
    Mass (Da):107,225
    Last modified:April 17, 2007 - v1
    Checksum:i12FF0306677286CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU207211 Genomic DNA. Translation: CAL61033.1.
    RefSeqiWP_011870373.1. NC_009138.1.
    YP_001099160.1. NC_009138.1.

    Genome annotation databases

    EnsemblBacteriaiCAL61033; CAL61033; HEAR0845.
    GeneIDi4931905.
    KEGGihar:HEAR0845.
    PATRICi22111792. VBIHerArs17568_0780.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU207211 Genomic DNA. Translation: CAL61033.1 .
    RefSeqi WP_011870373.1. NC_009138.1.
    YP_001099160.1. NC_009138.1.

    3D structure databases

    ProteinModelPortali A4G3E6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204773.HEAR0845.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL61033 ; CAL61033 ; HEAR0845 .
    GeneIDi 4931905.
    KEGGi har:HEAR0845.
    PATRICi 22111792. VBIHerArs17568_0780.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HARS204773:GJCA-806-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ULPAs1.

    Entry informationi

    Entry nameiSYI_HERAR
    AccessioniPrimary (citable) accession number: A4G3E6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3