A4G005 (RIFK_METM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	MmarC5_1492

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.
Sequence caution	The sequence ABO35789.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Riboflavin kinase HAMAP MF_01285		PRO_0000322072
Regions
	Nucleotide binding	10 – 15	6	CDP By similarity
	Nucleotide binding	109 – 112	4	CDP By similarity
Sites
	Metal binding	39	1	Magnesium By similarity
	Metal binding	41	1	Magnesium By similarity
	Binding site	96	1	FMN By similarity
	Binding site	104	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4G005 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 689D65F259C22AFA	FASTA	127	14,362
	10 20 30 40 50 60 MEIFGNVVSG LGEGRFFVGL TPYKNKFEEL TGFIPFEGTL NVKLKHNFNL DNFNPIEFDG 70 80 90 100 110 120 FEINGKRYFG GKVLLIKLFN KHGNFVNCAI VAPKKTDHSK KTLEIIAPIQ LRKFLSLNNS NVVKIVI

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000609 Genomic DNA. Translation: ABO35789.1. Different initiation.
RefSeq	YP_001098003.1. NC_009135.1.
3D structure databases
ProteinModelPortal	A4G005.
SMR	A4G005. Positions 1-127.
ModBase	Search...
Protein-protein interaction databases
STRING	A4G005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4927757.
GenomeReviews	Gene locus MmarC5_1492 in contig CP000609_GR.
KEGG	mmq:MmarC5_1492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG11680.
HOGENOM	HBG553349.
ProtClustDB	PRK14132.
Enzyme and pathway databases
BioCyc	MMAR402880:MMARC5_1492-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METM5

Accession

Primary (citable) accession number: A4G005

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program