Reviewed, UniProtKB/Swiss-Prot A4FZY2 (G1PDH_METM5)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	MmarC5_1469

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350653
Regions
	Nucleotide binding	77 – 81	5	NAD By similarity
	Nucleotide binding	99 – 102	4	NAD By similarity
Sites
	Metal binding	147	1	Zinc; catalytic By similarity
	Metal binding	225	1	Zinc; catalytic By similarity
	Metal binding	246	1	Zinc; catalytic By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FZY2-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 590E3E25E4651870	FASTA	334	36,831
	10 20 30 40 50 60 MIVIPRYTII KEKASSRIPE ILDNLNLKNP LVITGKNTKK YNKDFDFIYY DEIETSDLEN 70 80 90 100 110 120 IKNYANDYDS IMGIGGGRPI DIGKLIAHKS KKPFLSVPTT ASNDGIASPI VSLTQPSYMT 130 140 150 160 170 180 EAPIAIIADI GIIKKSPKKL LSAGMGDIVS NITAVLDWEL GKIEKSEKYS DSSGIFSKTI 190 200 210 220 230 240 AIELMDYVLN SNLEEYPKKL VKALIGSGIS IAIAHSSRPA SGSEHLFSHA LDIMKEKYDI 250 260 270 280 290 300 DTDSLHGEQC GVGTLAIAQI YFEEKKLDIE TFEMIKKSLK AVDAPITAKQ LGFDDEIVIE 310 320 330 ALSSAHALRN RHTILRNGIS KTEARKILEK SEII

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000609 Genomic DNA. Translation: ABO35766.1.
RefSeq	YP_001097980.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4FZY2.
Genome annotation databases
GeneID	4927633.
GenomeReviews	Gene locus MmarC5_1469 in contig CP000609_GR.
KEGG	mmq:MmarC5_1469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YTAVLDW.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. IPR016205. Glycerol_DH. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METM5

Accession

Primary (citable) accession number: A4FZY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	April 17, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)