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A4FZB0 (PUR2_METM5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:MmarC5_1246
OrganismMethanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]
Taxonomic identifier402880 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018824

Regions

Domain109 – 324216ATP-grasp
Nucleotide binding140 – 20263ATP By similarity

Sites

Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 2 By similarity
Metal binding2961Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4FZB0 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: B61CAD4435796E34

FASTA44448,696
        10         20         30         40         50         60 
MKVLLIGGGA REHAIAMALK KNELVELYTL MKNKNPGIYA ISDEVSFNSE TDVPAIKEFA 

        70         80         90        100        110        120 
EKIKPELAVI GPEAPLGVGA ADLLIEMGIP TVGPKKLPAQ IETSKEFMRN LFKKYEIDGS 

       130        140        150        160        170        180 
LRYAAFNEYG NDLETFIDEM TSLGKDVVVK PAGLTGGKGV KVVGEQLKDN EEAKIYAKEV 

       190        200        210        220        230        240 
FDKSIGGGNI IIEEKLVGVE FTLHGFVDGE NIVFMPAVQD HPHAYNNDEG PITGGMGSYS 

       250        260        270        280        290        300 
CPNHGLPFLS AEMLDRAEKI MEKTVSSINS EVGPYNGFLY GQFMLTADGP KIIEYNARFG 

       310        320        330        340        350        360 
DPEAMNLLPI LKTDFLDVCF AIAEGKLDKI NLEFENKATV CKYVVPNGYP IDPVRNKELT 

       370        380        390        400        410        420 
VDEKAIENAD AILFYASINE KNGKLYITGS RSAAVVGISE NIEEAEKIAQ MAIENFKGEV 

       430        440 
YYRSDIGTLG LIKKRIERVK QLAK 

« Hide

References

[1]"Complete sequence of chromosome of Methanococcus maripaludis C5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000609 Genomic DNA. Translation: ABO35544.1.
RefSeqYP_001097758.1. NC_009135.1.

3D structure databases

ProteinModelPortalA4FZB0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402880.MmarC5_1246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO35544; ABO35544; MmarC5_1246.
GeneID4929077.
KEGGmmq:MmarC5_1246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAFIDEYDG.

Enzyme and pathway databases

BioCycMMAR402880:GJ2R-1261-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METM5
AccessionPrimary (citable) accession number: A4FZB0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways