A4FZB0 (PUR2_METM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	MmarC5_1246

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018824
Regions
	Domain	109 – 324	216	ATP-grasp
	Nucleotide binding	140 – 202	63	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese 1 By similarity
	Metal binding	294	1	Magnesium or manganese 1 By similarity
	Metal binding	294	1	Magnesium or manganese 2 By similarity
	Metal binding	296	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FZB0 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: B61CAD4435796E34	FASTA	444	48,696
	10 20 30 40 50 60 MKVLLIGGGA REHAIAMALK KNELVELYTL MKNKNPGIYA ISDEVSFNSE TDVPAIKEFA 70 80 90 100 110 120 EKIKPELAVI GPEAPLGVGA ADLLIEMGIP TVGPKKLPAQ IETSKEFMRN LFKKYEIDGS 130 140 150 160 170 180 LRYAAFNEYG NDLETFIDEM TSLGKDVVVK PAGLTGGKGV KVVGEQLKDN EEAKIYAKEV 190 200 210 220 230 240 FDKSIGGGNI IIEEKLVGVE FTLHGFVDGE NIVFMPAVQD HPHAYNNDEG PITGGMGSYS 250 260 270 280 290 300 CPNHGLPFLS AEMLDRAEKI MEKTVSSINS EVGPYNGFLY GQFMLTADGP KIIEYNARFG 310 320 330 340 350 360 DPEAMNLLPI LKTDFLDVCF AIAEGKLDKI NLEFENKATV CKYVVPNGYP IDPVRNKELT 370 380 390 400 410 420 VDEKAIENAD AILFYASINE KNGKLYITGS RSAAVVGISE NIEEAEKIAQ MAIENFKGEV 430 440 YYRSDIGTLG LIKKRIERVK QLAK

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000609 Genomic DNA. Translation: ABO35544.1.
RefSeq	YP_001097758.1. NC_009135.1.
3D structure databases
ProteinModelPortal	A4FZB0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FZB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4929077.
GenomeReviews	Gene locus MmarC5_1246 in contig CP000609_GR.
KEGG	mmq:MmarC5_1246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04186.
HOGENOM	HBG404060.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	MMAR402880:MMARC5_1246-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METM5

Accession

Primary (citable) accession number: A4FZB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program