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A4FYA3 (SYL_METM5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MmarC5_0879
OrganismMethanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]
Taxonomic identifier402880 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length955 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 955955Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334844

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00049
Motif647 – 6515"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6501ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4FYA3 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: FF89DC413B300682

FASTA955110,927
        10         20         30         40         50         60 
MDQNGVNEGT GHKSVDLIQI MDKWQKKWTE AKIFEAEHDL RDKFFITAAF PYLNGVLHAG 

        70         80         90        100        110        120 
HLRTFTIPET IARYQRMKNK NVLWTFGFHV TGTPILGLAN QIKDRKEDII WAYNNLHNIP 

       130        140        150        160        170        180 
MDELLKLKTP EAIVECFSKK ATEAFKRMGF SLDWRRNFKT DDKVFSKFIE WQFYKLKEMG 

       190        200        210        220        230        240 
HITKGSHPVR YCPKCENPVE DHDLLHGEES TTVEYSLIKF TSEFDGKEII MPMATLRPET 

       250        260        270        280        290        300 
LFGVTNAWVN PNEIYVMAKV HDEIQKLDGE DVELKYNGIW IVGKECADKL KEQDRKIEIL 

       310        320        330        340        350        360 
KEIKGNELLG LKIKNPVTKK EVPLLPADFV EMGIGTGWVM SVPAHAPYDY VALRDLGKIE 

       370        380        390        400        410        420 
EVGLIPLIEI EGYDKYPAKE IVEKLGVKDQ NDDELLEQAT SKIYKDEFHK GKLNENCGEY 

       430        440        450        460        470        480 
AGISVKDIKE KLTKDYLNSN IAEIMYEFSE QKVVCRCGEK CIIKTVKGQW FINYSDENWK 

       490        500        510        520        530        540 
KLAHECIDNM NFAPENIRQE FHNKVDWMKD KACARKKGLG TILPFDENWI IESLSDSTIY 

       550        560        570        580        590        600 
MAYYTIARFI NEGLTPEQLI PELFEYVYLG NGNVEEITKN SNISKETIEE MRKEFLYYYP 

       610        620        630        640        650        660 
LDWRCSAKDL IPNHLTFMIF NHVALFGREH WPRGIEINGY VTIEGKKLSK SKGPVLPVSE 

       670        680        690        700        710        720 
VAENFGADVA RFYITTCAEL PQDADVKFKE MEKARDNLIK LYELAVSVME EEKTEKELSL 

       730        740        750        760        770        780 
IDKWLLHKTY SSINGAETAY EEFQLRKIGL MFYELINDLR WYKRRGGENN NVLKEVVEIW 

       790        800        810        820        830        840 
TKLLSPVTPH LCEEIWEKLG YAGFISQELY PEIKLEMVNE DLELGEEFIK SAMEDIRNIN 

       850        860        870        880        890        900 
GVAKINPEKM YLYTADDWKY DLLEFMNENS EKNVKALIPM VMKEDKFKRH GKEVMKLINE 

       910        920        930        940        950 
IMKIGVKKAI AEVEILENAK TFIESEFDCE VIVNGEDVKG KKKFAIPYKP AIYME 

« Hide

References

[1]"Complete sequence of chromosome of Methanococcus maripaludis C5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000609 Genomic DNA. Translation: ABO35187.1.
RefSeqYP_001097402.1. NC_009135.1.

3D structure databases

ProteinModelPortalA4FYA3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402880.MmarC5_0879.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO35187; ABO35187; MmarC5_0879.
GeneID4929284.
KEGGmmq:MmarC5_0879.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAGFYEGVM.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycMMAR402880:GJ2R-893-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METM5
AccessionPrimary (citable) accession number: A4FYA3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries