Reviewed, UniProtKB/Swiss-Prot A4FY69 (ASPD_METM5)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	MmarC5_0845

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H(2)O + NAD(P)(+) = oxaloacetate + NH(3) + NAD(P)H.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Probable L-aspartate dehydrogenase		PRO_1000067306
Sites
	Active site	218	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	190	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FY69-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: F8CCA8263E10E6D1	FASTA	267	28,716
	10 20 30 40 50 60 MLKVGVVGCG AIASLITKAL MSDRLNKAEV LAFYDGNLEK AEKLAMETGA DFCRSLDELV 70 80 90 100 110 120 SKDLDLIVEC ASVTAVEDTV IKSLNNGKDV IIMSVGAFAD KDLFLKLYKL AEKLERKIYI 130 140 150 160 170 180 PSGAVAGIDA VKSGSLGKIS DVTLTTTKPV HGLKNALEEQ GLNTDEIKEP KTVFEGTVFE 190 200 210 220 230 240 AISKFPQNIN VSVVLSLASR YPAKVKIIAD PCAVVNRHEI FVKGSIGTIK TCVENNPCRD 250 260 NPKTSALAAY SVIRLIKDLS EPIRIGT

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000609 Genomic DNA. Translation: ABO35153.1.
RefSeq	YP_001097368.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4929041.
GenomeReviews	Gene locus MmarC5_0845 in contig CP000609_GR.
KEGG	mmq:MmarC5_0845.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DHase_NAD-bd. IPR002811. Asp_DHase. IPR011182. Asp_DHase_NAD_syn. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_METM5

Accession

Primary (citable) accession number: A4FY69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 17, 2007
Last modified:	November 25, 2008
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)