A4FY69 (ASPD_METM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	MmarC5_0845

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067306
Sites
	Active site	218	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	190	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FY69 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: F8CCA8263E10E6D1	FASTA	267	28,716
	10 20 30 40 50 60 MLKVGVVGCG AIASLITKAL MSDRLNKAEV LAFYDGNLEK AEKLAMETGA DFCRSLDELV 70 80 90 100 110 120 SKDLDLIVEC ASVTAVEDTV IKSLNNGKDV IIMSVGAFAD KDLFLKLYKL AEKLERKIYI 130 140 150 160 170 180 PSGAVAGIDA VKSGSLGKIS DVTLTTTKPV HGLKNALEEQ GLNTDEIKEP KTVFEGTVFE 190 200 210 220 230 240 AISKFPQNIN VSVVLSLASR YPAKVKIIAD PCAVVNRHEI FVKGSIGTIK TCVENNPCRD 250 260 NPKTSALAAY SVIRLIKDLS EPIRIGT

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000609 Genomic DNA. Translation: ABO35153.1.
RefSeq	YP_001097368.1. NC_009135.1.
3D structure databases
ProteinModelPortal	A4FY69.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FY69.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4929041.
GenomeReviews	Gene locus MmarC5_0845 in contig CP000609_GR.
KEGG	mmq:MmarC5_0845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05209.
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13304.
Enzyme and pathway databases
BioCyc	MMAR402880:MMARC5_0845-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_METM5

Accession

Primary (citable) accession number: A4FY69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program