Reviewed, UniProtKB/Swiss-Prot A4FY19 (DNLI_METM5)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MmarC5_0793

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	DNA ligase		PRO_1000049870
Sites
	Active site	252	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FY19-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: BC74B0A7585AECA8	FASTA	573	65,893
	10 20 30 40 50 60 MLFNDFCKIL DKIEKTTKRL EKTDYFVELI DFIKTNGKPE NLKQVSQITI GRVFAEFENK 70 80 90 100 110 120 EIGIGPNLLL EAVKTTGISE KDLKAEIKKT GDIGTAVENL SSNIKQVSLF SQPLTLEEMY 130 140 150 160 170 180 STLKKLSEIE GNSSQKKKIR IISNLLILAN PVESRYISRL ILEDMRIGMN IPTILASFSN 190 200 210 220 230 240 YFNVNKENVE KIYAVTNDIG LLGEKLISGS DIENDSELHL KVFRPIKPML AQLTPSIEDA 250 260 270 280 290 300 IIETKIPQFE TKYDGARVQV HKSNGEVKIY SRRLEDITNS VPELVEEIKK IDIDNIILEG 310 320 330 340 350 360 ECVAMDLSSG KPRPFQDILR RFRRKYDINK MAEKIALRIY FFDVLYYNRG LIDTPLRNRR 370 380 390 400 410 420 EILEKLFGTN DWDTELEKIE KEILSKKMLF SSFKLNSDDP NLAKEFFNWS LSIGHEGVMI 430 440 450 460 470 480 KNPDAPYTPG SRVKTMYKVK PTLENLDVVV TRAKIGMGKR KDWYGSYEIS VKDDESNLHV 490 500 510 520 530 540 IGNVGSGLTE DDLERLTKIV NEIKIEDLGE EVILEPKIVL EVTYEEIQTS EKYEMGYALR 550 560 570 FPRVVQIRED KSINDINTLD DVKKIYDIER NRK

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000609 Genomic DNA. Translation: ABO35103.1.
RefSeq	YP_001097318.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4928716.
GenomeReviews	Gene locus MmarC5_0793 in contig CP000609_GR.
KEGG	mmq:MmarC5_0793.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METM5

Accession

Primary (citable) accession number: A4FY19

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)