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A4FWU0

- BIOB_METM5

UniProt

A4FWU0 - BIOB_METM5

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Protein

Biotin synthase

Gene

bioB

Organism
Methanococcus maripaludis (strain C5 / ATCC BAA-1333)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi277 – 2771Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMMAR402880:GJ2R-364-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:MmarC5_0353
OrganismiMethanococcus maripaludis (strain C5 / ATCC BAA-1333)
Taxonomic identifieri402880 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000253: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Biotin synthasePRO_0000381463Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi402880.MmarC5_0353.

Structurei

3D structure databases

ProteinModelPortaliA4FWU0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCIARITL.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FWU0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEIKLNSDS LEIYEKSASK KLNRNDLIDL WNLDLNDLLD ISYALKKLFN
60 70 80 90 100
KDKIDLCSIM NAKSGICPEN CIFCSQSKHN SSKIDTYELK SKEEILKNAK
110 120 130 140 150
SVEKYSNRFS IVVSGKTVTD LEFEKIIESI EEIQNKTKLK VCVSLGLLNK
160 170 180 190 200
DQLKALNEKN VRIHNNLETS ENYFKNICTT HDYNDKIKVI LEAKKIGLEM
210 220 230 240 250
CSGGIFGMGE SIDDRINLFL DLKKLGVDSI ALNLLNPIYG TKIYDRINSG
260 270 280 290 300
DISPINSIGA LKSLCIARIT LPNKVIRLCG GREHVLKDMQ KYSLLAIDGL
310 320
MIGNYLTTNG QNIQSDLKMI EEMGFER
Length:327
Mass (Da):36,989
Last modified:April 17, 2007 - v1
Checksum:i1404478603B267D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000609 Genomic DNA. Translation: ABO34669.1.
RefSeqiWP_011868124.1. NC_009135.1.
YP_001096884.1. NC_009135.1.

Genome annotation databases

EnsemblBacteriaiABO34669; ABO34669; MmarC5_0353.
GeneIDi4928010.
KEGGimmq:MmarC5_0353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000609 Genomic DNA. Translation: ABO34669.1 .
RefSeqi WP_011868124.1. NC_009135.1.
YP_001096884.1. NC_009135.1.

3D structure databases

ProteinModelPortali A4FWU0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402880.MmarC5_0353.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO34669 ; ABO34669 ; MmarC5_0353 .
GeneIDi 4928010.
KEGGi mmq:MmarC5_0353.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CIARITL.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MMAR402880:GJ2R-364-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C5 / ATCC BAA-1333.

Entry informationi

Entry nameiBIOB_METM5
AccessioniPrimary (citable) accession number: A4FWU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 17, 2007
Last modified: October 1, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3