A4FWH0 (A4FWH0_METM5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	MmarC5_0229

Organism

Methanococcus maripaludis (strain C5 / ATCC BAA-1333) [Complete proteome] [HAMAP]

Taxonomic identifier

402880 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ABO34545.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	36	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A4FWH0 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: F047234F5C5A0C4F	FASTA	258	28,632
	10 20 30 40 50 60 MIRTNEQLEE LANNIRDFIR EQVENADAKG VVVGLSGGID SALVAYLSVK ALGKENVFGV 70 80 90 100 110 120 IMPEKNSNPD DEKYGKLVAE TLGINYTVFD ITPVLVAFGA GGYVEGKEFD RRVDANLKPR 130 140 150 160 170 180 IRMTEVYYHA NKKNYLVAGT SNKSEIYMGY GTKYGDLGSD FLTIGNLFKT EVRQLAGHVG 190 200 210 220 230 240 VLKEIIDKAP SAGLWDGQTD EDELGISYET LDKLLDLMEK GKEIEDICEF LGISLEKIEE 250 LMLRITTNKH KSLPLPMP

References

[1]

"Complete sequence of chromosome of Methanococcus maripaludis C5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Detter J.C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C5 / ATCC BAA-1333.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000609 Genomic DNA. Translation: ABO34545.1.
RefSeq	YP_001096760.1. NC_009135.1.
3D structure databases
ProteinModelPortal	A4FWH0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FWH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4929019.
GenomeReviews	Gene locus MmarC5_0229 in contig CP000609_GR.
KEGG	mmq:MmarC5_0229.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	A4FWH0.
ProtClustDB	CLSK876543.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4FWH0_METM5

Accession

Primary (citable) accession number: A4FWH0

Entry history

Integrated into UniProtKB/TrEMBL:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)