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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanococcus maripaludis (strain C5 / ATCC BAA-1333)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADUniRule annotation1
Binding sitei67NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei133NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi161Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi176IronUniRule annotation1
Binding sitei179NAD; via carbonyl oxygenUniRule annotation1
Binding sitei226NAD; via amide nitrogenUniRule annotation1
Binding sitei236GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 60NADUniRule annotation2
Nucleotide bindingi159 – 161NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMMAR402880:G1G8G-229-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MmarC5_0226
OrganismiMethanococcus maripaludis (strain C5 / ATCC BAA-1333)
Taxonomic identifieri402880 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000253 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001156101 – 261Thiamine thiazole synthaseAdd BLAST261

Proteomic databases

PRIDEiA4FWG7

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi402880.MmarC5_0226

Structurei

3D structure databases

ProteinModelPortaliA4FWG7
SMRiA4FWG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

A4FWG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGKLRADEV AVTKSILKST FNMWMDVIDV DVVIVGAGPS GLTAAKYLAQ
60 70 80 90 100
KGVKTVVLER HLSFGGGTWG GGMGFPNIVV EKPADEILRE AGIKLDEVDG
110 120 130 140 150
EDELFTADSV EVPAKLGVAA IDAGAKILTG IVVEDLILKE DKIAGVVIQS
160 170 180 190 200
YAIEKAGLHI DPLTISAKYV IDSTGHDASA VHTLARKNKD LGIEVPGEKS
210 220 230 240 250
MWAEKGENSL TRNTREIFPG LYVCGMAANA YHAGYRMGAI FGGMYLSGKK
260
CAEMILEKME K
Length:261
Mass (Da):27,857
Last modified:April 17, 2007 - v1
Checksum:iE455DED3D4B522B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000609 Genomic DNA Translation: ABO34542.1
RefSeqiWP_011868000.1, NC_009135.1

Genome annotation databases

EnsemblBacteriaiABO34542; ABO34542; MmarC5_0226
GeneIDi4928881
KEGGimmq:MmarC5_0226

Similar proteinsi

Entry informationi

Entry nameiTHI4_METM5
AccessioniPrimary (citable) accession number: A4FWG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 17, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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