A4FUN7 (UBP12_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12A
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 12A
Ubiquitin thioesterase 12A
Ubiquitin-specific-processing protease 12A

Gene names

Name:	usp12a
Synonyms:	usp12

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. USP12/USP46 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Ubiquitin carboxyl-terminal hydrolase 12A		PRO_0000378991
Sites
	Active site	48	1	Nucleophile By similarity
	Active site	318	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FUN7 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 930E27CEBE0C8A53	FASTA	371	43,027
	10 20 30 40 50 60 MEILMTVSKF ASFCTMGANA SALEKEIGSE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC 70 80 90 100 110 120 RPFREKILAY RSQPRRKENL LTCLADLFHS IANQKRKVGV IPPKKFITRL RKENELFDNY 130 140 150 160 170 180 MQQDAHEFLN YLLNTIADLL QEERKQDKQN GKLANGTLDS QNNNSTPPST TWVHEIFQGT 190 200 210 220 230 240 LTNETRCLTC ETISSKDEDF LDLSVDVEQN TSITHCLRGF SNTETLCSEY KYYCEECRSK 250 260 270 280 290 300 QEAHKRMRVK KLPMILALHL KRFKYMEQLQ RYTKLSYRVV FPLELRLFNT SGDATNPERL 310 320 330 340 350 360 YDLVAVVVHC GSGPNRGHYI AIVKSHDFWL LFDDDIVEKI DAQAIEEFYG LTSEISKNSE 370 SGYILFYQSR D

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC115286 mRNA. Translation: AAI15287.1.
IPI	IPI00508425.
RefSeq	NP_001077025.1. NM_001083556.1.
UniGene	Dr.5972.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000112670; ENSDARP00000103890; ENSDARG00000078109.
GeneID	565736.
KEGG	dre:565736.
Organism-specific databases
CTD	565736.
ZFIN	ZDB-GENE-060228-3. usp12a.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00650000093027.
HOGENOM	HOG000231498.
KO	K11842.
OMA	SKFASFC.
OrthoDB	EOG42Z4Q8.
Gene expression databases
Bgee	A4FUN7.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20886545.

Entry information

Entry name

UBP12_DANRE

Accession

Primary (citable) accession number: A4FUN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	April 17, 2007
Last modified:	May 16, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program