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Protein

Ubiquitin carboxyl-terminal hydrolase 12A

Gene

usp12a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481NucleophilePROSITE-ProRule annotation
Active sitei318 – 3181Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12A
Ubiquitin thioesterase 12A
Ubiquitin-specific-processing protease 12A
Gene namesi
Name:usp12a
Synonyms:usp12
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 24

Organism-specific databases

ZFINiZDB-GENE-060228-3. usp12a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Ubiquitin carboxyl-terminal hydrolase 12APRO_0000378991Add
BLAST

Expressioni

Gene expression databases

BgeeiA4FUN7.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000103890.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 370332USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
InParanoidiA4FUN7.
KOiK11842.
OMAiNENNSPP.
OrthoDBiEOG7RBZ8G.
PhylomeDBiA4FUN7.
TreeFamiTF314144.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FUN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEILMTVSKF ASFCTMGANA SALEKEIGSE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKILAY RSQPRRKENL LTCLADLFHS IANQKRKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADLL QEERKQDKQN
160 170 180 190 200
GKLANGTLDS QNNNSTPPST TWVHEIFQGT LTNETRCLTC ETISSKDEDF
210 220 230 240 250
LDLSVDVEQN TSITHCLRGF SNTETLCSEY KYYCEECRSK QEAHKRMRVK
260 270 280 290 300
KLPMILALHL KRFKYMEQLQ RYTKLSYRVV FPLELRLFNT SGDATNPERL
310 320 330 340 350
YDLVAVVVHC GSGPNRGHYI AIVKSHDFWL LFDDDIVEKI DAQAIEEFYG
360 370
LTSEISKNSE SGYILFYQSR D
Length:371
Mass (Da):43,027
Last modified:April 17, 2007 - v1
Checksum:i930E27CEBE0C8A53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC115286 mRNA. Translation: AAI15287.1.
RefSeqiNP_001077025.1. NM_001083556.1.
UniGeneiDr.5972.

Genome annotation databases

EnsembliENSDART00000112670; ENSDARP00000103890; ENSDARG00000078109.
GeneIDi565736.
KEGGidre:565736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC115286 mRNA. Translation: AAI15287.1.
RefSeqiNP_001077025.1. NM_001083556.1.
UniGeneiDr.5972.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000103890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000112670; ENSDARP00000103890; ENSDARG00000078109.
GeneIDi565736.
KEGGidre:565736.

Organism-specific databases

CTDi565736.
ZFINiZDB-GENE-060228-3. usp12a.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
InParanoidiA4FUN7.
KOiK11842.
OMAiNENNSPP.
OrthoDBiEOG7RBZ8G.
PhylomeDBiA4FUN7.
TreeFamiTF314144.

Miscellaneous databases

NextBioi20886545.
PROiA4FUN7.

Gene expression databases

BgeeiA4FUN7.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBP12_DANRE
AccessioniPrimary (citable) accession number: A4FUN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 17, 2007
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.