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Protein

Putative oxidoreductase GLYR1

Gene

GLYR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei362 – 3621NADBy similarity
Binding sitei505 – 5051NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi168 – 18013A.T hookAdd
BLAST
Nucleotide bindingi271 – 28515NADBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

DNA-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Putative oxidoreductase GLYR1 (EC:1.-.-.-)
Alternative name(s):
Glyoxylate reductase 1 homolog
Nuclear protein NP60
Gene namesi
Name:GLYR1
Synonyms:NP60
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Putative oxidoreductase GLYR1PRO_0000312120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301PhosphoserineBy similarity
Cross-linki135 – 135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei167 – 1671PhosphoserineBy similarity
Cross-linki227 – 227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki237 – 237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki269 – 269Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei540 – 5401PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA4FUF0.
PRIDEiA4FUF0.

Expressioni

Gene expression databases

ExpressionAtlasiA4FUF0. baseline.

Interactioni

Subunit structurei

Interacts with MAPK14.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002268.

Structurei

3D structure databases

ProteinModelPortaliA4FUF0.
SMRiA4FUF0. Positions 262-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6659PWWPPROSITE-ProRule annotationAdd
BLAST

Domaini

The A.T hook DNA-binding domain is required for the interaction with MAPK14.By similarity
The PWWP domain probably mediates the binding to H3K36me3.By similarity

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
KOG1904. Eukaryota.
COG2084. LUCA.
HOGENOMiHOG000219609.
InParanoidiA4FUF0.
OMAiHAMTDIE.
OrthoDBiEOG7992RZ.
TreeFamiTF324195.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR000313. PWWP_dom.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A4FUF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVSLRLGD LVWGKLGRYP PWPGKIVNPP KDLKKPRGKK CFFVKFFGTE
60 70 80 90 100
DHAWIKVEQL KPYHAHKEEM IKINKGKRFQ QAVDAVEEFL RRAKGKDQTS
110 120 130 140 150
SHSSADDKNR RNSSEERSRP ISGDEKRKLS LSEGKVKKNM GEGKKRVPSG
160 170 180 190 200
SSERGSKSPL KRAQEQSPRK RGRPPKDEKD LSIPESSTVK GMMAGPMATF
210 220 230 240 250
KWQPNVSEPV KDADPHFHHF LLSQTEKPAV CYQAITKKLK ICEEETGSTS
260 270 280 290 300
IQAADSTAVN GSVTPTDKKI GFLGLGLMGS GIVSNLLKMG HTVTVWNRTA
310 320 330 340 350
EKCDLFIQEG ARLGRTPAEV VSTCDITFAC VSDPKAAKDL VLGPSGVLQG
360 370 380 390 400
IRPGKCYVDM STVDADTVTE LAQVIVSRGG RFLEAPVSGN QQLSNDGMLV
410 420 430 440 450
ILAAGDRGLY EDCSSCFQAM GKTSFFLGEV GNAAKMMLIV NMVQGSFMAT
460 470 480 490 500
IAEGLTLAQV TGQSQQTLLD ILNQGQLASI FLDQKCQNIL QGNFKPDFYL
510 520 530 540 550
KYIQKDLRLA IALGDAVNHP TPMAAAANEV YKRAKALDQS DNDMSAVYRA

YIH
Length:553
Mass (Da):60,556
Last modified:April 17, 2007 - v1
Checksum:i7B07769AE69EC46E
GO
Isoform 2 (identifier: A4FUF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-553: Missing.

Note: No experimental confirmation available.
Show »
Length:269
Mass (Da):30,133
Checksum:iF1772E89758120CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei270 – 553284Missing in isoform 2. 1 PublicationVSP_029705Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021674 mRNA. Translation: AAX46521.1.
BC114770 mRNA. Translation: AAI14771.1.
RefSeqiNP_001035658.2. NM_001040568.2. [A4FUF0-1]
UniGeneiBt.58736.

Genome annotation databases

EnsembliENSBTAT00000002268; ENSBTAP00000002268; ENSBTAG00000001731. [A4FUF0-1]
GeneIDi539636.
KEGGibta:539636.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021674 mRNA. Translation: AAX46521.1.
BC114770 mRNA. Translation: AAI14771.1.
RefSeqiNP_001035658.2. NM_001040568.2. [A4FUF0-1]
UniGeneiBt.58736.

3D structure databases

ProteinModelPortaliA4FUF0.
SMRiA4FUF0. Positions 262-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002268.

Proteomic databases

PaxDbiA4FUF0.
PRIDEiA4FUF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002268; ENSBTAP00000002268; ENSBTAG00000001731. [A4FUF0-1]
GeneIDi539636.
KEGGibta:539636.

Organism-specific databases

CTDi84656.

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
KOG1904. Eukaryota.
COG2084. LUCA.
HOGENOMiHOG000219609.
InParanoidiA4FUF0.
OMAiHAMTDIE.
OrthoDBiEOG7992RZ.
TreeFamiTF324195.

Gene expression databases

ExpressionAtlasiA4FUF0. baseline.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR000313. PWWP_dom.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiGLYR1_BOVIN
AccessioniPrimary (citable) accession number: A4FUF0
Secondary accession number(s): Q58DC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: April 17, 2007
Last modified: June 8, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved NAD-binding sites and sequence similarity to plant dehydrogenases suggest that this protein may have oxidoreductase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.