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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi345 – 3528ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Gene namesi
Name:MCM3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 808807DNA replication licensing factor MCM3PRO_0000318902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei160 – 1601PhosphoserineBy similarity
Modified residuei275 – 2751PhosphoserineBy similarity
Modified residuei293 – 2931N6-acetyllysineBy similarity
Modified residuei535 – 5351Phosphoserine; by ATMBy similarity
Modified residuei547 – 5471N6-acetyllysineBy similarity
Modified residuei611 – 6111PhosphoserineBy similarity
Modified residuei668 – 6681PhosphoserineBy similarity
Modified residuei672 – 6721PhosphoserineBy similarity
Modified residuei681 – 6811PhosphoserineBy similarity
Modified residuei708 – 7081PhosphotyrosineBy similarity
Modified residuei713 – 7131PhosphothreonineBy similarity
Modified residuei722 – 7221PhosphothreonineBy similarity
Modified residuei728 – 7281PhosphoserineBy similarity
Modified residuei734 – 7341PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA4FUD9.
PRIDEiA4FUD9.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP (By similarity). Interacts with ANKRD17.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014194.

Structurei

3D structure databases

ProteinModelPortaliA4FUD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini295 – 502208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi477 – 4804Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiA4FUD9.
KOiK02541.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4FUD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN
60 70 80 90 100
VNDLRRKNEK RANRLLSNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYI
110 120 130 140 150
GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA
160 170 180 190 200
TKKTIERRYS DLTSLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQIIT
210 220 230 240 250
IQEMPEKAPA GQLPRSVDVI LDDDLVDRVK PGDRVQVVGT YRCLPGKKGG
260 270 280 290 300
YTSGTFRTVL IACNVKQMSK DVQPSFSAED IAKIKKFSKT RSKDIFDQLA
310 320 330 340 350
RSLAPSIHGH DYVKKAILCL LLGGVERDLE NGSHIRGDIN ILLIGDPSVA
360 370 380 390 400
KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL
410 420 430 440 450
ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA
460 470 480 490 500
AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH
510 520 530 540 550
VLRMHRYRAP GEQDGDAMPL GSAVDILATD DPNFSPDDQQ DTQIYEKHDN
560 570 580 590 600
LLHGIKKKKE KMVSAAFMRK YIHVAKIIKP VLTQESAAYI AEEYSRLRSQ
610 620 630 640 650
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ
660 670 680 690 700
YAYFKKVLEK EKKRKKRSED ESDAEDEVEK SQEDQEQKTK RRRICPSDAK
710 720 730 740 750
EGDSYDPYDF TNTEEEMPQV HTPKATDSQE TKESQKVELS ESRLKAFKAA
760 770 780 790 800
LLEVFREAHA QSVGMNRLTE SVNRDNEEPF SSAEIQAALS RMQDDNQVMV

SEGIVFLI
Length:808
Mass (Da):90,858
Last modified:April 17, 2007 - v1
Checksum:i4344BE9A95678C9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114737 mRNA. Translation: AAI14738.1.
RefSeqiNP_001013604.2. NM_001013586.2.
UniGeneiBt.8503.

Genome annotation databases

GeneIDi281302.
KEGGibta:281302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114737 mRNA. Translation: AAI14738.1.
RefSeqiNP_001013604.2. NM_001013586.2.
UniGeneiBt.8503.

3D structure databases

ProteinModelPortaliA4FUD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014194.

Proteomic databases

PaxDbiA4FUD9.
PRIDEiA4FUD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281302.
KEGGibta:281302.

Organism-specific databases

CTDi4172.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiA4FUD9.
KOiK02541.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiMCM3_BOVIN
AccessioniPrimary (citable) accession number: A4FUD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: April 17, 2007
Last modified: July 6, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.