Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A4FR54 (SYL_SACEN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SACE_7373
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length955 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 955955Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334809

Regions

Motif66 – 7712"HIGH" region HAMAP-Rule MF_00049
Motif725 – 7295"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4FR54 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 37394D241D975782

FASTA955107,406
        10         20         30         40         50         60 
MSGQAEGSTN TEEVPRFRYT AQTAAEIEQR WQRRWEELGT FHAPNPAGSL KGEVSDEKLF 

        70         80         90        100        110        120 
VQDMFPYPSG SGLHVGHPLG FIGTDVYARF NRMLGKNVLH TMGFDSFGLP AEQYAVQTGT 

       130        140        150        160        170        180 
HPRTTTEKNI ERYLTQIRRL GLGHDERRRV ATTDIPFYRW TQWIFLQIFH SWYDTDADRA 

       190        200        210        220        230        240 
RPISELEAQF AAGERATPDG RPWAELSRTE QRRIIDSYRL VYLSEAPVNW APGLGTVVAN 

       250        260        270        280        290        300 
EEVTADGLTE RGNFPVFRRN LKQWMMRITA YADRLIDDLD RLDWPDKIKT MQRNWIGRSQ 

       310        320        330        340        350        360 
GANVVFPLDG SAGSIEVFTT RPDTLFGVTY LVLAPEHPLV DELTAAQWPQ DPDLRWTGGA 

       370        380        390        400        410        420 
ATPAEAVAQY RRAASMKSDL DRQENKEKTG VFTGAWATNP VNGEQVPVFI ADYVLMGYGT 

       430        440        450        460        470        480 
GAIMAVPGED QRDFDFAEAF GLPVVRTVQP SEGFEGGAYS GEGPRINSAN PDVGLDLNGM 

       490        500        510        520        530        540 
HLDEAKKTII EWLESHKHGS GTVQYKLRDW LFARQRYWGE PFPVVYDSDG IPLGLPESEL 

       550        560        570        580        590        600 
PVVLPEVADY SPRTFDPEDA DSRPEPPLAK ATEWANVELD LGDGLKNYER DTNVMPQWAG 

       610        620        630        640        650        660 
SCWYQLRYID PDNDQAFVDP ANERYWMGKR PELHGPDDPG GLDLYIGGVE HGVLHLLYSR 

       670        680        690        700        710        720 
FWHKVLYDLG HVSSEEPYRR LYNQGYIQAY AYTDSRGVYV PAEEVEERDG KFFFQGEEVR 

       730        740        750        760        770        780 
REYGKMGKSL KNSVSPDEMA DAYGADTLRL YEMAMGPLDA SRPWATKDVV GSHRFLQRLW 

       790        800        810        820        830        840 
RNVVDENTGE LRVTDDEPAI EVLRALHKTI AGVREDYREL RFNTAVAKLI ELNNLLTKEY 

       850        860        870        880        890        900 
SATGAPRAVV GPLVLMVAPL APHMAEELWS KLGHDGSLAH GPFPEADEQY LVEDTVEYPI 

       910        920        930        940        950 
QFNGKVRSRI VVPASAGQDE VKAAALADEK VVAALDGREP RKVIVVPGRL VNVVG 

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM420293 Genomic DNA. Translation: CAM06529.1.
RefSeqYP_001109454.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FR54.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405948.SACE_7373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM06529; CAM06529; SACE_7373.
GeneID4947103.
KEGGsen:SACE_7373.
PATRIC23422238. VBISacEry28377_7393.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAKGNPLES.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_SACEN
AccessionPrimary (citable) accession number: A4FR54
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries