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A4FR54

- SYL_SACEN

UniProt

A4FR54 - SYL_SACEN

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Protein
Leucine--tRNA ligase
Gene
leuS, SACE_7373
Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei728 – 7281ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:SACE_7373
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 955955Leucine--tRNA ligaseUniRule annotation
PRO_0000334809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_7373.

Structurei

3D structure databases

ProteinModelPortaliA4FR54.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7712"HIGH" regionUniRule annotation
Add
BLAST
Motifi725 – 7295"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiKGNPLES.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FR54-1 [UniParc]FASTAAdd to Basket

« Hide

MSGQAEGSTN TEEVPRFRYT AQTAAEIEQR WQRRWEELGT FHAPNPAGSL    50
KGEVSDEKLF VQDMFPYPSG SGLHVGHPLG FIGTDVYARF NRMLGKNVLH 100
TMGFDSFGLP AEQYAVQTGT HPRTTTEKNI ERYLTQIRRL GLGHDERRRV 150
ATTDIPFYRW TQWIFLQIFH SWYDTDADRA RPISELEAQF AAGERATPDG 200
RPWAELSRTE QRRIIDSYRL VYLSEAPVNW APGLGTVVAN EEVTADGLTE 250
RGNFPVFRRN LKQWMMRITA YADRLIDDLD RLDWPDKIKT MQRNWIGRSQ 300
GANVVFPLDG SAGSIEVFTT RPDTLFGVTY LVLAPEHPLV DELTAAQWPQ 350
DPDLRWTGGA ATPAEAVAQY RRAASMKSDL DRQENKEKTG VFTGAWATNP 400
VNGEQVPVFI ADYVLMGYGT GAIMAVPGED QRDFDFAEAF GLPVVRTVQP 450
SEGFEGGAYS GEGPRINSAN PDVGLDLNGM HLDEAKKTII EWLESHKHGS 500
GTVQYKLRDW LFARQRYWGE PFPVVYDSDG IPLGLPESEL PVVLPEVADY 550
SPRTFDPEDA DSRPEPPLAK ATEWANVELD LGDGLKNYER DTNVMPQWAG 600
SCWYQLRYID PDNDQAFVDP ANERYWMGKR PELHGPDDPG GLDLYIGGVE 650
HGVLHLLYSR FWHKVLYDLG HVSSEEPYRR LYNQGYIQAY AYTDSRGVYV 700
PAEEVEERDG KFFFQGEEVR REYGKMGKSL KNSVSPDEMA DAYGADTLRL 750
YEMAMGPLDA SRPWATKDVV GSHRFLQRLW RNVVDENTGE LRVTDDEPAI 800
EVLRALHKTI AGVREDYREL RFNTAVAKLI ELNNLLTKEY SATGAPRAVV 850
GPLVLMVAPL APHMAEELWS KLGHDGSLAH GPFPEADEQY LVEDTVEYPI 900
QFNGKVRSRI VVPASAGQDE VKAAALADEK VVAALDGREP RKVIVVPGRL 950
VNVVG 955
Length:955
Mass (Da):107,406
Last modified:April 17, 2007 - v1
Checksum:i37394D241D975782
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1.
RefSeqiYP_001109454.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06529; CAM06529; SACE_7373.
GeneIDi4947103.
KEGGisen:SACE_7373.
PATRICi23422238. VBISacEry28377_7393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1 .
RefSeqi YP_001109454.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FR54.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405948.SACE_7373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM06529 ; CAM06529 ; SACE_7373 .
GeneIDi 4947103.
KEGGi sen:SACE_7373.
PATRICi 23422238. VBISacEry28377_7393.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi KGNPLES.
OrthoDBi EOG63Z74X.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiSYL_SACEN
AccessioniPrimary (citable) accession number: A4FR54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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