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A4FR54

- SYL_SACEN

UniProt

A4FR54 - SYL_SACEN

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei728 – 7281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:SACE_7373
    OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
    Taxonomic identifieri405948 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
    ProteomesiUP000006728: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 955955Leucine--tRNA ligasePRO_0000334809Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi405948.SACE_7373.

    Structurei

    3D structure databases

    ProteinModelPortaliA4FR54.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7712"HIGH" regionAdd
    BLAST
    Motifi725 – 7295"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiKGNPLES.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4FR54-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGQAEGSTN TEEVPRFRYT AQTAAEIEQR WQRRWEELGT FHAPNPAGSL    50
    KGEVSDEKLF VQDMFPYPSG SGLHVGHPLG FIGTDVYARF NRMLGKNVLH 100
    TMGFDSFGLP AEQYAVQTGT HPRTTTEKNI ERYLTQIRRL GLGHDERRRV 150
    ATTDIPFYRW TQWIFLQIFH SWYDTDADRA RPISELEAQF AAGERATPDG 200
    RPWAELSRTE QRRIIDSYRL VYLSEAPVNW APGLGTVVAN EEVTADGLTE 250
    RGNFPVFRRN LKQWMMRITA YADRLIDDLD RLDWPDKIKT MQRNWIGRSQ 300
    GANVVFPLDG SAGSIEVFTT RPDTLFGVTY LVLAPEHPLV DELTAAQWPQ 350
    DPDLRWTGGA ATPAEAVAQY RRAASMKSDL DRQENKEKTG VFTGAWATNP 400
    VNGEQVPVFI ADYVLMGYGT GAIMAVPGED QRDFDFAEAF GLPVVRTVQP 450
    SEGFEGGAYS GEGPRINSAN PDVGLDLNGM HLDEAKKTII EWLESHKHGS 500
    GTVQYKLRDW LFARQRYWGE PFPVVYDSDG IPLGLPESEL PVVLPEVADY 550
    SPRTFDPEDA DSRPEPPLAK ATEWANVELD LGDGLKNYER DTNVMPQWAG 600
    SCWYQLRYID PDNDQAFVDP ANERYWMGKR PELHGPDDPG GLDLYIGGVE 650
    HGVLHLLYSR FWHKVLYDLG HVSSEEPYRR LYNQGYIQAY AYTDSRGVYV 700
    PAEEVEERDG KFFFQGEEVR REYGKMGKSL KNSVSPDEMA DAYGADTLRL 750
    YEMAMGPLDA SRPWATKDVV GSHRFLQRLW RNVVDENTGE LRVTDDEPAI 800
    EVLRALHKTI AGVREDYREL RFNTAVAKLI ELNNLLTKEY SATGAPRAVV 850
    GPLVLMVAPL APHMAEELWS KLGHDGSLAH GPFPEADEQY LVEDTVEYPI 900
    QFNGKVRSRI VVPASAGQDE VKAAALADEK VVAALDGREP RKVIVVPGRL 950
    VNVVG 955
    Length:955
    Mass (Da):107,406
    Last modified:April 17, 2007 - v1
    Checksum:i37394D241D975782
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM06529.1.
    RefSeqiYP_001109454.1. NC_009142.1.

    Genome annotation databases

    EnsemblBacteriaiCAM06529; CAM06529; SACE_7373.
    GeneIDi4947103.
    KEGGisen:SACE_7373.
    PATRICi23422238. VBISacEry28377_7393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM06529.1 .
    RefSeqi YP_001109454.1. NC_009142.1.

    3D structure databases

    ProteinModelPortali A4FR54.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 405948.SACE_7373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM06529 ; CAM06529 ; SACE_7373 .
    GeneIDi 4947103.
    KEGGi sen:SACE_7373.
    PATRICi 23422238. VBISacEry28377_7393.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi KGNPLES.
    OrthoDBi EOG63Z74X.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
      Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
      Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NRRL 23338.

    Entry informationi

    Entry nameiSYL_SACEN
    AccessioniPrimary (citable) accession number: A4FR54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3