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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei728 – 7281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SACE_7373
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 955955Leucine--tRNA ligasePRO_0000334809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_7373.

Structurei

3D structure databases

ProteinModelPortaliA4FR54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7712"HIGH" regionAdd
BLAST
Motifi725 – 7295"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FR54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQAEGSTN TEEVPRFRYT AQTAAEIEQR WQRRWEELGT FHAPNPAGSL
60 70 80 90 100
KGEVSDEKLF VQDMFPYPSG SGLHVGHPLG FIGTDVYARF NRMLGKNVLH
110 120 130 140 150
TMGFDSFGLP AEQYAVQTGT HPRTTTEKNI ERYLTQIRRL GLGHDERRRV
160 170 180 190 200
ATTDIPFYRW TQWIFLQIFH SWYDTDADRA RPISELEAQF AAGERATPDG
210 220 230 240 250
RPWAELSRTE QRRIIDSYRL VYLSEAPVNW APGLGTVVAN EEVTADGLTE
260 270 280 290 300
RGNFPVFRRN LKQWMMRITA YADRLIDDLD RLDWPDKIKT MQRNWIGRSQ
310 320 330 340 350
GANVVFPLDG SAGSIEVFTT RPDTLFGVTY LVLAPEHPLV DELTAAQWPQ
360 370 380 390 400
DPDLRWTGGA ATPAEAVAQY RRAASMKSDL DRQENKEKTG VFTGAWATNP
410 420 430 440 450
VNGEQVPVFI ADYVLMGYGT GAIMAVPGED QRDFDFAEAF GLPVVRTVQP
460 470 480 490 500
SEGFEGGAYS GEGPRINSAN PDVGLDLNGM HLDEAKKTII EWLESHKHGS
510 520 530 540 550
GTVQYKLRDW LFARQRYWGE PFPVVYDSDG IPLGLPESEL PVVLPEVADY
560 570 580 590 600
SPRTFDPEDA DSRPEPPLAK ATEWANVELD LGDGLKNYER DTNVMPQWAG
610 620 630 640 650
SCWYQLRYID PDNDQAFVDP ANERYWMGKR PELHGPDDPG GLDLYIGGVE
660 670 680 690 700
HGVLHLLYSR FWHKVLYDLG HVSSEEPYRR LYNQGYIQAY AYTDSRGVYV
710 720 730 740 750
PAEEVEERDG KFFFQGEEVR REYGKMGKSL KNSVSPDEMA DAYGADTLRL
760 770 780 790 800
YEMAMGPLDA SRPWATKDVV GSHRFLQRLW RNVVDENTGE LRVTDDEPAI
810 820 830 840 850
EVLRALHKTI AGVREDYREL RFNTAVAKLI ELNNLLTKEY SATGAPRAVV
860 870 880 890 900
GPLVLMVAPL APHMAEELWS KLGHDGSLAH GPFPEADEQY LVEDTVEYPI
910 920 930 940 950
QFNGKVRSRI VVPASAGQDE VKAAALADEK VVAALDGREP RKVIVVPGRL

VNVVG
Length:955
Mass (Da):107,406
Last modified:April 17, 2007 - v1
Checksum:i37394D241D975782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1.
RefSeqiWP_009945755.1. NZ_ABFV01000023.1.
YP_001109454.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06529; CAM06529; SACE_7373.
KEGGisen:SACE_7373.
PATRICi23422238. VBISacEry28377_7393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1.
RefSeqiWP_009945755.1. NZ_ABFV01000023.1.
YP_001109454.1. NC_009142.1.

3D structure databases

ProteinModelPortaliA4FR54.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SACE_7373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM06529; CAM06529; SACE_7373.
KEGGisen:SACE_7373.
PATRICi23422238. VBISacEry28377_7393.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiSYL_SACEN
AccessioniPrimary (citable) accession number: A4FR54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: April 29, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.