Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A4FR54

- SYL_SACEN

UniProt

A4FR54 - SYL_SACEN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei728 – 7281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SACE_7373
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 955955Leucine--tRNA ligasePRO_0000334809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_7373.

Structurei

3D structure databases

ProteinModelPortaliA4FR54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7712"HIGH" regionAdd
BLAST
Motifi725 – 7295"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiKGNPLES.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FR54-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGQAEGSTN TEEVPRFRYT AQTAAEIEQR WQRRWEELGT FHAPNPAGSL
60 70 80 90 100
KGEVSDEKLF VQDMFPYPSG SGLHVGHPLG FIGTDVYARF NRMLGKNVLH
110 120 130 140 150
TMGFDSFGLP AEQYAVQTGT HPRTTTEKNI ERYLTQIRRL GLGHDERRRV
160 170 180 190 200
ATTDIPFYRW TQWIFLQIFH SWYDTDADRA RPISELEAQF AAGERATPDG
210 220 230 240 250
RPWAELSRTE QRRIIDSYRL VYLSEAPVNW APGLGTVVAN EEVTADGLTE
260 270 280 290 300
RGNFPVFRRN LKQWMMRITA YADRLIDDLD RLDWPDKIKT MQRNWIGRSQ
310 320 330 340 350
GANVVFPLDG SAGSIEVFTT RPDTLFGVTY LVLAPEHPLV DELTAAQWPQ
360 370 380 390 400
DPDLRWTGGA ATPAEAVAQY RRAASMKSDL DRQENKEKTG VFTGAWATNP
410 420 430 440 450
VNGEQVPVFI ADYVLMGYGT GAIMAVPGED QRDFDFAEAF GLPVVRTVQP
460 470 480 490 500
SEGFEGGAYS GEGPRINSAN PDVGLDLNGM HLDEAKKTII EWLESHKHGS
510 520 530 540 550
GTVQYKLRDW LFARQRYWGE PFPVVYDSDG IPLGLPESEL PVVLPEVADY
560 570 580 590 600
SPRTFDPEDA DSRPEPPLAK ATEWANVELD LGDGLKNYER DTNVMPQWAG
610 620 630 640 650
SCWYQLRYID PDNDQAFVDP ANERYWMGKR PELHGPDDPG GLDLYIGGVE
660 670 680 690 700
HGVLHLLYSR FWHKVLYDLG HVSSEEPYRR LYNQGYIQAY AYTDSRGVYV
710 720 730 740 750
PAEEVEERDG KFFFQGEEVR REYGKMGKSL KNSVSPDEMA DAYGADTLRL
760 770 780 790 800
YEMAMGPLDA SRPWATKDVV GSHRFLQRLW RNVVDENTGE LRVTDDEPAI
810 820 830 840 850
EVLRALHKTI AGVREDYREL RFNTAVAKLI ELNNLLTKEY SATGAPRAVV
860 870 880 890 900
GPLVLMVAPL APHMAEELWS KLGHDGSLAH GPFPEADEQY LVEDTVEYPI
910 920 930 940 950
QFNGKVRSRI VVPASAGQDE VKAAALADEK VVAALDGREP RKVIVVPGRL

VNVVG
Length:955
Mass (Da):107,406
Last modified:April 17, 2007 - v1
Checksum:i37394D241D975782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1.
RefSeqiYP_001109454.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06529; CAM06529; SACE_7373.
GeneIDi4947103.
KEGGisen:SACE_7373.
PATRICi23422238. VBISacEry28377_7393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06529.1 .
RefSeqi YP_001109454.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FR54.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405948.SACE_7373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM06529 ; CAM06529 ; SACE_7373 .
GeneIDi 4947103.
KEGGi sen:SACE_7373.
PATRICi 23422238. VBISacEry28377_7393.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi KGNPLES.
OrthoDBi EOG63Z74X.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiSYL_SACEN
AccessioniPrimary (citable) accession number: A4FR54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3