Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
Metal bindingi227 – 2271ManganeseUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation
Active sitei271 – 2711UniRule annotation
Metal bindingi294 – 2941ManganeseUniRule annotation
Binding sitei386 – 3861GTPUniRule annotation
Binding sitei417 – 4171GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTPUniRule annotation
Nucleotide bindingi513 – 5164GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SACE_7274
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_1000060299Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009635.

Structurei

3D structure databases

ProteinModelPortaliA4FQV7.
SMRiA4FQV7. Positions 12-604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FQV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE
60 70 80 90 100
QLVEAGTFKR LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW
110 120 130 140 150
MQPDEMKAIM TELYRGCMRG RTMYVIPFCM GPLDADKPKL GVEITDTAYV
160 170 180 190 200
VVSMHIMTRM GSKVLERLGE DEDFVEALHS VGAPLEPGQQ DVPWPCNDTK
210 220 230 240 250
YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD EGWLAEHMLI
260 270 280 290 300
LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF
310 320 330 340 350
GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG
360 370 380 390 400
DVWWEEMEGE PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE
410 420 430 440 450
WDDPNGVPIS AILFGGRRKT TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA
460 470 480 490 500
GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN VGKEADSSKL PRIFYVNWFR
510 520 530 540 550
RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR VPSADQIDLS
560 570 580 590 600
GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK

QRLGA
Length:605
Mass (Da):67,840
Last modified:April 17, 2007 - v1
Checksum:i7735D145A4F6F3F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiWP_011875298.1. NZ_ABFV01000014.1.
YP_001109357.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiWP_011875298.1. NZ_ABFV01000014.1.
YP_001109357.1. NC_009142.1.

3D structure databases

ProteinModelPortaliA4FQV7.
SMRiA4FQV7. Positions 12-604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiPCKG_SACEN
AccessioniPrimary (citable) accession number: A4FQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: June 24, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.