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A4FQV7 (PCKG_SACEN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:SACE_7274
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000060299

Regions

Nucleotide binding270 – 2756GTP By similarity
Nucleotide binding513 – 5164GTP By similarity
Region384 – 3863Substrate binding By similarity

Sites

Active site2711 By similarity
Metal binding2271Manganese By similarity
Metal binding2471Manganese; via tele nitrogen By similarity
Metal binding2941Manganese By similarity
Binding site791Substrate By similarity
Binding site2201Substrate; via amide nitrogen By similarity
Binding site2271Substrate By similarity
Binding site2691Substrate By similarity
Binding site3861GTP By similarity
Binding site4171GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4FQV7 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 7735D145A4F6F3F4

FASTA60567,840
        10         20         30         40         50         60 
MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE QLVEAGTFKR 

        70         80         90        100        110        120 
LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW MQPDEMKAIM TELYRGCMRG 

       130        140        150        160        170        180 
RTMYVIPFCM GPLDADKPKL GVEITDTAYV VVSMHIMTRM GSKVLERLGE DEDFVEALHS 

       190        200        210        220        230        240 
VGAPLEPGQQ DVPWPCNDTK YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD 

       250        260        270        280        290        300 
EGWLAEHMLI LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF 

       310        320        330        340        350        360 
GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG DVWWEEMEGE 

       370        380        390        400        410        420 
PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE WDDPNGVPIS AILFGGRRKT 

       430        440        450        460        470        480 
TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN 

       490        500        510        520        530        540 
VGKEADSSKL PRIFYVNWFR RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR 

       550        560        570        580        590        600 
VPSADQIDLS GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK 


QRLGA 

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqYP_001109357.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FQV7.
SMRA4FQV7. Positions 12-604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405948.SACE_7274.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM06432; CAM06432; SACE_7274.
GeneID4947006.
KEGGsen:SACE_7274.
PATRIC23422038. VBISacEry28377_7292.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWDNPAGV.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_SACEN
AccessionPrimary (citable) accession number: A4FQV7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways