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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateUniRule annotation1
Metal bindingi227ManganeseUniRule annotation1
Metal bindingi247Manganese; via tele nitrogenUniRule annotation1
Binding sitei269SubstrateUniRule annotation1
Active sitei271UniRule annotation1
Metal bindingi294ManganeseUniRule annotation1
Binding sitei386GTPUniRule annotation1
Binding sitei417GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi270 – 275GTPUniRule annotation6
Nucleotide bindingi513 – 516GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SACE_7274
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000602991 – 605Phosphoenolpyruvate carboxykinase [GTP]Add BLAST605

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009635.

Structurei

3D structure databases

ProteinModelPortaliA4FQV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni384 – 386Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FQV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE
60 70 80 90 100
QLVEAGTFKR LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW
110 120 130 140 150
MQPDEMKAIM TELYRGCMRG RTMYVIPFCM GPLDADKPKL GVEITDTAYV
160 170 180 190 200
VVSMHIMTRM GSKVLERLGE DEDFVEALHS VGAPLEPGQQ DVPWPCNDTK
210 220 230 240 250
YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD EGWLAEHMLI
260 270 280 290 300
LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF
310 320 330 340 350
GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG
360 370 380 390 400
DVWWEEMEGE PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE
410 420 430 440 450
WDDPNGVPIS AILFGGRRKT TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA
460 470 480 490 500
GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN VGKEADSSKL PRIFYVNWFR
510 520 530 540 550
RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR VPSADQIDLS
560 570 580 590 600
GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK

QRLGA
Length:605
Mass (Da):67,840
Last modified:April 17, 2007 - v1
Checksum:i7735D145A4F6F3F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiWP_011875298.1. NZ_ABFV01000014.1.

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiWP_011875298.1. NZ_ABFV01000014.1.

3D structure databases

ProteinModelPortaliA4FQV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_SACEN
AccessioniPrimary (citable) accession number: A4FQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: October 5, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.