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A4FQV7

- PCKG_SACEN

UniProt

A4FQV7 - PCKG_SACEN

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
Metal bindingi227 – 2271ManganeseUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation
Active sitei271 – 2711UniRule annotation
Metal bindingi294 – 2941ManganeseUniRule annotation
Binding sitei386 – 3861GTPUniRule annotation
Binding sitei417 – 4171GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTPUniRule annotation
Nucleotide bindingi513 – 5164GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SACE_7274
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_1000060299Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SACE_7274.

Structurei

3D structure databases

ProteinModelPortaliA4FQV7.
SMRiA4FQV7. Positions 12-604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FQV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE
60 70 80 90 100
QLVEAGTFKR LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW
110 120 130 140 150
MQPDEMKAIM TELYRGCMRG RTMYVIPFCM GPLDADKPKL GVEITDTAYV
160 170 180 190 200
VVSMHIMTRM GSKVLERLGE DEDFVEALHS VGAPLEPGQQ DVPWPCNDTK
210 220 230 240 250
YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD EGWLAEHMLI
260 270 280 290 300
LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF
310 320 330 340 350
GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG
360 370 380 390 400
DVWWEEMEGE PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE
410 420 430 440 450
WDDPNGVPIS AILFGGRRKT TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA
460 470 480 490 500
GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN VGKEADSSKL PRIFYVNWFR
510 520 530 540 550
RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR VPSADQIDLS
560 570 580 590 600
GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK

QRLGA
Length:605
Mass (Da):67,840
Last modified:April 17, 2007 - v1
Checksum:i7735D145A4F6F3F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiYP_001109357.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
GeneIDi4947006.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1 .
RefSeqi YP_001109357.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FQV7.
SMRi A4FQV7. Positions 12-604.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405948.SACE_7274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM06432 ; CAM06432 ; SACE_7274 .
GeneIDi 4947006.
KEGGi sen:SACE_7274.
PATRICi 23422038. VBISacEry28377_7292.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiPCKG_SACEN
AccessioniPrimary (citable) accession number: A4FQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3