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A4FQV7

- PCKG_SACEN

UniProt

A4FQV7 - PCKG_SACEN

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
pckG, SACE_7274
Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Substrate By similarity
Binding sitei220 – 2201Substrate; via amide nitrogen By similarity
Metal bindingi227 – 2271Manganese By similarity
Binding sitei227 – 2271Substrate By similarity
Metal bindingi247 – 2471Manganese; via tele nitrogen By similarity
Binding sitei269 – 2691Substrate By similarity
Active sitei271 – 2711 By similarity
Metal bindingi294 – 2941Manganese By similarity
Binding sitei386 – 3861GTP By similarity
Binding sitei417 – 4171GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTP By similarity
Nucleotide bindingi513 – 5164GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:SACE_7274
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_1000060299Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SACE_7274.

Structurei

3D structure databases

ProteinModelPortaliA4FQV7.
SMRiA4FQV7. Positions 12-604.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FQV7-1 [UniParc]FASTAAdd to Basket

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MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE    50
QLVEAGTFKR LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW 100
MQPDEMKAIM TELYRGCMRG RTMYVIPFCM GPLDADKPKL GVEITDTAYV 150
VVSMHIMTRM GSKVLERLGE DEDFVEALHS VGAPLEPGQQ DVPWPCNDTK 200
YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD EGWLAEHMLI 250
LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF 300
GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG 350
DVWWEEMEGE PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE 400
WDDPNGVPIS AILFGGRRKT TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA 450
GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN VGKEADSSKL PRIFYVNWFR 500
RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR VPSADQIDLS 550
GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK 600
QRLGA 605
Length:605
Mass (Da):67,840
Last modified:April 17, 2007 - v1
Checksum:i7735D145A4F6F3F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1.
RefSeqiYP_001109357.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
GeneIDi4947006.
KEGGisen:SACE_7274.
PATRICi23422038. VBISacEry28377_7292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM06432.1 .
RefSeqi YP_001109357.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FQV7.
SMRi A4FQV7. Positions 12-604.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405948.SACE_7274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM06432 ; CAM06432 ; SACE_7274 .
GeneIDi 4947006.
KEGGi sen:SACE_7274.
PATRICi 23422038. VBISacEry28377_7292.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiPCKG_SACEN
AccessioniPrimary (citable) accession number: A4FQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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