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A4FQV7

- PCKG_SACEN

UniProt

A4FQV7 - PCKG_SACEN

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791SubstrateUniRule annotation
    Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
    Metal bindingi227 – 2271ManganeseUniRule annotation
    Binding sitei227 – 2271SubstrateUniRule annotation
    Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
    Binding sitei269 – 2691SubstrateUniRule annotation
    Active sitei271 – 2711UniRule annotation
    Metal bindingi294 – 2941ManganeseUniRule annotation
    Binding sitei386 – 3861GTPUniRule annotation
    Binding sitei417 – 4171GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi270 – 2756GTPUniRule annotation
    Nucleotide bindingi513 – 5164GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:SACE_7274
    OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
    Taxonomic identifieri405948 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
    ProteomesiUP000006728: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 605605Phosphoenolpyruvate carboxykinase [GTP]PRO_1000060299Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi405948.SACE_7274.

    Structurei

    3D structure databases

    ProteinModelPortaliA4FQV7.
    SMRiA4FQV7. Positions 12-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni384 – 3863Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4FQV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSLTIPGLD QAPTTHARLL SWVREVAELT TPDRVVWCDG SQDEWQRLTE    50
    QLVEAGTFKR LEKKPNSFYA ASDPSDVARV EERTFICSRE EKDAGVTNYW 100
    MQPDEMKAIM TELYRGCMRG RTMYVIPFCM GPLDADKPKL GVEITDTAYV 150
    VVSMHIMTRM GSKVLERLGE DEDFVEALHS VGAPLEPGQQ DVPWPCNDTK 200
    YITHFPEERK IWSFGSGYGG NALLGKKCFS LRIASAMARD EGWLAEHMLI 250
    LKLISPEDKV HYVAAAFPSA CGKTNLAMLQ PTIPGWRVES LGDDIAWMRF 300
    GEDGRLYAVN PEAGFFGVAP GTNWKTNPNA MRTIDQGNSL FTNVALTDDG 350
    DVWWEEMEGE PQHLTDWKGR DWTPQSDEKA AHPNSRYCTP MSQCPILAPE 400
    WDDPNGVPIS AILFGGRRKT TIPLVNEAFD WQHGVFMGAT LSSEKTAAAA 450
    GKVGEVRRDP MAMLPFIGYN VGDYFQHWVN VGKEADSSKL PRIFYVNWFR 500
    RDESGKKIVW PGFGENSRVL KWIVERLDGN AAAEDTPIGR VPSADQIDLS 550
    GLDTPREDVE TALHVDVEEW KAELPLIEEW FASIGDSLPS SMRDEFEALK 600
    QRLGA 605
    Length:605
    Mass (Da):67,840
    Last modified:April 17, 2007 - v1
    Checksum:i7735D145A4F6F3F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM06432.1.
    RefSeqiYP_001109357.1. NC_009142.1.

    Genome annotation databases

    EnsemblBacteriaiCAM06432; CAM06432; SACE_7274.
    GeneIDi4947006.
    KEGGisen:SACE_7274.
    PATRICi23422038. VBISacEry28377_7292.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM06432.1 .
    RefSeqi YP_001109357.1. NC_009142.1.

    3D structure databases

    ProteinModelPortali A4FQV7.
    SMRi A4FQV7. Positions 12-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 405948.SACE_7274.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM06432 ; CAM06432 ; SACE_7274 .
    GeneIDi 4947006.
    KEGGi sen:SACE_7274.
    PATRICi 23422038. VBISacEry28377_7292.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
      Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
      Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NRRL 23338.

    Entry informationi

    Entry nameiPCKG_SACEN
    AccessioniPrimary (citable) accession number: A4FQV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3